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External Quality Assessment for the Detection of Measles Virus by Reverse Transcription-PCR Using Armored RNA
Zhang, Dong1,2,3; Sun, Yu1; Jia, Tingting1,2,3; Zhang, Lei1,4; Wang, Guojing1,2,3; Zhang, Rui1; Zhang, Kuo1; Lin, Guigao1; Xie, Jiehong1; Wang, Lunan1,2,3; Li, Jinming1,2,3
刊名PLOS ONE
2015-08-05
DOI10.1371/journal.pone.0134681
10期:8
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]SURVEILLANCE ; ELIMINATION ; STANDARDS ; PROGRESS ; RUBELLA ; CHINA
英文摘要

In recent years, nucleic acid tests for detection of measles virus RNA have been widely applied in laboratories belonging to the measles surveillance system of China. An external quality assessment program was established by the National Center for Clinical Laboratories to evaluate the performance of nucleic acid tests for measles virus. The external quality assessment panel, which consisted of 10 specimens, was prepared using armored RNAs, complex of noninfectious MS2 bacteriophage coat proteins encapsulated RNA of measles virus, as measles virus surrogate controls. Conserved sequences amplified from a circulating measles virus strain or from a vaccine strain were encapsulated into these armored RNAs. Forty-one participating laboratories from 15 provinces, municipalities, or autonomous regions that currently conduct molecular detection of measles virus enrolled in the external quality assessment program, including 40 measles surveillance system laboratories and one diagnostic reagent manufacturer. Forty laboratories used commercial reverse transcription-quantitative PCR kits, with only one laboratory applying a conventional PCR method developed in-house. The results indicated that most of the participants (38/41, 92.7%) were able to accurately detect the panel with 100% sensitivity and 100% specificity. Although a wide range of commercially available kits for nucleic acid extraction and reverse transcription polymerase chain reaction were used by the participants, only two false-negative results and one false-positive result were generated; these were generated by three separate laboratories. Both false-negative results were obtained with tests performed on specimens with the lowest concentration (1.2 x 10(4) genomic equivalents/mL). In addition, all 18 participants from Beijing achieved 100% sensitivity and 100% specificity. Overall, we conclude that the majority of the laboratories evaluated have reliable diagnostic capacities for the detection of measles virus.

语种英语
WOS记录号WOS:000359061400098
引用统计
被引频次:7[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/123097
专题北京大学医学部管理机构_研究生院
作者单位1.Beijing Hosp, Natl Ctr Clin Labs, Beijing, Peoples R China
2.Chinese Acad Med Sci, Grad Sch, Beijing 100730, Peoples R China
3.Peking Union Med Coll, Beijing 100021, Peoples R China
4.Peking Univ, Hlth Sci Ctr, Grad Sch, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Zhang, Dong,Sun, Yu,Jia, Tingting,et al. External Quality Assessment for the Detection of Measles Virus by Reverse Transcription-PCR Using Armored RNA[J]. PLOS ONE,2015,10(8).
APA Zhang, Dong.,Sun, Yu.,Jia, Tingting.,Zhang, Lei.,Wang, Guojing.,...&Li, Jinming.(2015).External Quality Assessment for the Detection of Measles Virus by Reverse Transcription-PCR Using Armored RNA.PLOS ONE,10(8).
MLA Zhang, Dong,et al."External Quality Assessment for the Detection of Measles Virus by Reverse Transcription-PCR Using Armored RNA".PLOS ONE 10.8(2015).
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