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Multinucleation and cell dysfunction induced by amorphous silica nanoparticles in an L-02 human hepatic cell line
Wang, Wen1,2,3; Li, Yang1,2,3; Liu, Xiaomei3; Jin, Minghua3; Du, Haiying3; Liu, Ying3; Huang, Peili1,2; Zhou, Xianqing1,2; Yuan, Lan4; Sun, Zhiwei1,2,3
关键词silica nanoparticles human hepatic cell L-02 multinucleation cell cycle cell dysfunction apoptosis
刊名INTERNATIONAL JOURNAL OF NANOMEDICINE
2013
DOI10.2147/IJN.S46732
8页:3533-3541
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Nanoscience & Nanotechnology ; Pharmacology & Pharmacy
资助者National Natural Science Foundation of China ; Innovative Team Project of Beijing Education Committee ; Doctoral Program of Higher Education ; National Natural Science Foundation of China ; Innovative Team Project of Beijing Education Committee ; Doctoral Program of Higher Education
研究领域[WOS]Science & Technology - Other Topics ; Pharmacology & Pharmacy
关键词[WOS]CHROMOSOME SEGREGATION ; ULTRAFINE PARTICLES ; DNA-DAMAGE ; CYTOTOXICITY ; SIZE ; CYTOKINESIS ; HEPATOCYTES
英文摘要

Silica nanoparticles (SNPs) are one of the most important nanomaterials, and have been widely used in a variety of fields. Therefore, their effects on human health and the environment have been addressed in a number of studies. In this work, the effects of amorphous SNPs were investigated with regard to multinucleation in L-02 human hepatic cells. Our results show that L-02 cells had an abnormally high incidence of multinucleation upon exposure to silica, that increased in a dose-dependent manner. Propidium iodide staining showed that multinucleated cells were arrested in G2/M phase of the cell cycle. Increased multinucleation in L-02 cells was associated with increased generation of cellular reactive oxygen species and mitochondrial damage on flow cytometry and confocal microscopy, which might have led to failure of cytokinesis in these cells. Further, SNPs inhibited cell growth and induced apoptosis in exposed cells. Taken together, our findings demonstrate that multinucleation in L-02 human hepatic cells might be a failure to undergo cytokinesis or cell fusion in response to SNPs, and the increase in cellular reactive oxygen species could be responsible for the apoptosis seen in both mononuclear cells and multinucleated cells.

语种英语
所属项目编号81172704 ; PHR201107116 ; 20090061110062
资助者National Natural Science Foundation of China ; Innovative Team Project of Beijing Education Committee ; Doctoral Program of Higher Education ; National Natural Science Foundation of China ; Innovative Team Project of Beijing Education Committee ; Doctoral Program of Higher Education
WOS记录号WOS:000324513800001
引用统计
被引频次:14[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/123408
专题北京大学医药卫生分析中心
作者单位1.Peking Univ, Med & Hlth Anal Ctr, Beijing, Peoples R China
2.Capital Med Univ, Sch Publ Hlth, Beijing 100069, Peoples R China
3.Capital Med Univ, Beijing Key Lab Environm Toxicol, Beijing 100069, Peoples R China
4.Jilin Univ, Sch Publ Hlth, Changchun 130023, Jilin, Peoples R China
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GB/T 7714
Wang, Wen,Li, Yang,Liu, Xiaomei,et al. Multinucleation and cell dysfunction induced by amorphous silica nanoparticles in an L-02 human hepatic cell line[J]. INTERNATIONAL JOURNAL OF NANOMEDICINE,2013,8:3533-3541.
APA Wang, Wen.,Li, Yang.,Liu, Xiaomei.,Jin, Minghua.,Du, Haiying.,...&Sun, Zhiwei.(2013).Multinucleation and cell dysfunction induced by amorphous silica nanoparticles in an L-02 human hepatic cell line.INTERNATIONAL JOURNAL OF NANOMEDICINE,8,3533-3541.
MLA Wang, Wen,et al."Multinucleation and cell dysfunction induced by amorphous silica nanoparticles in an L-02 human hepatic cell line".INTERNATIONAL JOURNAL OF NANOMEDICINE 8(2013):3533-3541.
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