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学科主题: 公共卫生
题名:
Multinucleation and cell dysfunction induced by amorphous silica nanoparticles in an L-02 human hepatic cell line
作者: Wang, Wen1,2,3; Li, Yang1,2,3; Liu, Xiaomei3; Jin, Minghua3; Du, Haiying3; Liu, Ying3; Huang, Peili1,2; Zhou, Xianqing1,2; Yuan, Lan4; Sun, Zhiwei1,2,3
关键词: silica nanoparticles ; human hepatic cell L-02 ; multinucleation ; cell cycle ; cell dysfunction ; apoptosis
刊名: INTERNATIONAL JOURNAL OF NANOMEDICINE
发表日期: 2013
DOI: 10.2147/IJN.S46732
卷: 8, 页:3533-3541
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Nanoscience & Nanotechnology ; Pharmacology & Pharmacy
研究领域[WOS]: Science & Technology - Other Topics ; Pharmacology & Pharmacy
关键词[WOS]: CHROMOSOME SEGREGATION ; ULTRAFINE PARTICLES ; DNA-DAMAGE ; CYTOTOXICITY ; SIZE ; CYTOKINESIS ; HEPATOCYTES
英文摘要:

Silica nanoparticles (SNPs) are one of the most important nanomaterials, and have been widely used in a variety of fields. Therefore, their effects on human health and the environment have been addressed in a number of studies. In this work, the effects of amorphous SNPs were investigated with regard to multinucleation in L-02 human hepatic cells. Our results show that L-02 cells had an abnormally high incidence of multinucleation upon exposure to silica, that increased in a dose-dependent manner. Propidium iodide staining showed that multinucleated cells were arrested in G2/M phase of the cell cycle. Increased multinucleation in L-02 cells was associated with increased generation of cellular reactive oxygen species and mitochondrial damage on flow cytometry and confocal microscopy, which might have led to failure of cytokinesis in these cells. Further, SNPs inhibited cell growth and induced apoptosis in exposed cells. Taken together, our findings demonstrate that multinucleation in L-02 human hepatic cells might be a failure to undergo cytokinesis or cell fusion in response to SNPs, and the increase in cellular reactive oxygen species could be responsible for the apoptosis seen in both mononuclear cells and multinucleated cells.

语种: 英语
所属项目编号: 81172704 ; PHR201107116 ; 20090061110062
项目资助者: National Natural Science Foundation of China ; Innovative Team Project of Beijing Education Committee ; Doctoral Program of Higher Education
WOS记录号: WOS:000324513800001
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/123408
Appears in Collections:北京大学医药卫生分析中心_期刊论文

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作者单位: 1.Peking Univ, Med & Hlth Anal Ctr, Beijing, Peoples R China
2.Capital Med Univ, Sch Publ Hlth, Beijing 100069, Peoples R China
3.Capital Med Univ, Beijing Key Lab Environm Toxicol, Beijing 100069, Peoples R China
4.Jilin Univ, Sch Publ Hlth, Changchun 130023, Jilin, Peoples R China

Recommended Citation:
Wang, Wen,Li, Yang,Liu, Xiaomei,et al. Multinucleation and cell dysfunction induced by amorphous silica nanoparticles in an L-02 human hepatic cell line[J]. INTERNATIONAL JOURNAL OF NANOMEDICINE,2013,8:3533-3541.
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