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学科主题: 公共卫生
题名:
The effects of amino acid replacements of glycine 20 on conformational stability and catalysis of staphylococcal nuclease
作者: Feng, YM; Huang, S; Zhang, WZ; Zeng, ZH; Zou, XJ; Zhong, LJ; Peng, JR; Jing, GZ
关键词: staphylococcal nuclease ; amino acid substitutions ; kinetic analysis ; conformational stability
刊名: BIOCHIMIE
发表日期: 2004-12-01
DOI: 10.1016/j.biochi.2004.10.005
卷: 86, 期:12, 页:893-901
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology
研究领域[WOS]: Biochemistry & Molecular Biology
关键词[WOS]: FLIGHT MASS-SPECTROMETRY ; LIMITED PROTEOLYSIS ; MAGNETIC-RESONANCE ; CRYSTAL-STRUCTURE ; NMR-SPECTROSCOPY ; MOLTEN GLOBULE ; PROTEIN ; STATE ; MUTANTS ; COMPLEX
英文摘要:

Staphylococcal nuclease (SNase) is a well-established model for protein folding studies. Its three-dimensional structure has been determined. The enzyme, Ca2+, and DNA or RNA substrate form a ternary complex. Glycine 20 is the second position of the first beta-turn of SNase, which may serve as the folding initiation site for the SNase polypeptide. To study the role of Gly20 in the conformational stability and catalysis of SNase, three mutants, in which Gly20 was replaced by alanine, valine, or isoleucine, were constructed and studied by using circular dichroism spectra, intrinsic and ANS-binding fluorescence spectra, stability and activity assays. The mutations have little effect on the conformational integrity of the mutants. However, the catalytic activity is reduced drastically by the mutations, and the stability of the protein is progressively decreased in the order G20A

语种: 英语
WOS记录号: WOS:000226885600004
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/124004
Appears in Collections:北京大学医药卫生分析中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, Beijing 100101, Peoples R China
2.Chinese Acad Sci, Inst Biophys, Ctr Struct & Mol Biol, Beijing 100101, Peoples R China
3.Peking Univ, Med & Hlth Analyt Ctr, Beijing 100871, Peoples R China

Recommended Citation:
Feng, YM,Huang, S,Zhang, WZ,et al. The effects of amino acid replacements of glycine 20 on conformational stability and catalysis of staphylococcal nuclease[J]. BIOCHIMIE,2004,86(12):893-901.
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