IR@PKUHSC  > 北京大学医药卫生分析中心
学科主题公共卫生
Two isomers of HDTIC isolated from Astragali Radix decrease the expression of p16 in 2BS cells
Wang Pei-Chang1; Zhang Zong-Yu1,2; Ran, Zhang; Tong Tan-Jun2
关键词HDTIC Astragali Radix replicative senescence fibroblast gene expression
刊名CHINESE MEDICAL JOURNAL
2008-02-05
121期:3页:231-235
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Medicine, General & Internal
研究领域[WOS]General & Internal Medicine
关键词[WOS]HUMAN-DIPLOID FIBROBLASTS ; REPLICATIVE SENESCENCE ; CELLULAR SENESCENCE ; P16(INK4A) EXPRESSION ; INHIBITORS ; DELAY ; REPERFUSION ; INVOLVEMENT ; P21(WAF1) ; APOPTOSIS
英文摘要

Background Astragali Radix, the root of Astragalus membranceus (Fish) Bunge Var. mongholicus (Bge), is a crude drug considered as one of the effective traditional Chinese anti-ageing material. The two isomers of 4-hydroxy-5-hydroxymethyl-[1,3]dioxolan-2,6′-spirane-5′,6′,7′,8′-tetrahydro-indolizine-3′-carbaldehyde (HDTIC), HDTIC-1 and HDTIC-2, were first extracted from the herb in 2002. We demonstrated previously that 0.1 mu mol/L HDTIC-1 or 1.0 mu mol/L HDTIC-2 strongly delay replicative senescence of human fetal lung diploid fibroblasts (2BS). In this study, we chose them to investigate their effects on the expression of senescence-associated genes to explore the mechanism of how HDTIC delays replicative senescence.

Methods The effects of HDTIC-1 and HDTIC-2 on the expression of p16 and p21 were observed in vitro by RT-PCR and Western blot. The anti-oxidative activities of the compounds were also observed by phenotype alteration after treatment with antioxidants. Results There was an obvious expression of p16 in the control senescent cells. However, in the 2BS cells, after 56 population doublings (PDs) grown from PD28 in 0.1 mu mol/L HDTIC-1 or 1.0 mu mol/L HDTIC-2, there was a weak mRNA expression of p16 and no protein expression of p16 was observed. The expression level of p2l increased with cell ageing. Moreover, there was no difference between the expression level of p21 in the control cells and that in the same PD cells cultured with HDTIC compounds. The results also showed that 2BS cells exposed to 100 mu mol/L H(2)O(2) for 5 minutes return to their non-senescent phenotype and continue to be confluent after incubating the damaged cells with HDTIC-1 (1.0 mu mol/L) or HDTIC-2 (10 mu mol/L) for 1 hour.

Conclusions Expression of p16 by 2BS cells was strongly inhibited by HDTIC compounds, which could contribute to their delayed replicative senescence by the way of p16(INK4a)/Rb/MAPK. The anti-oxidative activities of HDTIC-1 and HDTIC-2, described in this study for the first time, might be indirectly related to their inhibition of p16 expression.

语种英语
WOS记录号WOS:000253116600009
引用统计
被引频次:8[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/124331
专题北京大学医药卫生分析中心
作者单位1.Capital Med Univ, Xuanwu Hosp, Dept Lab Med, Beijing 100053, Peoples R China
2.Peking Univ, Med & Hlth Ctr, Ageing Researching Ctr, Beijing 100083, Peoples R China
推荐引用方式
GB/T 7714
Wang Pei-Chang,Zhang Zong-Yu,Ran, Zhang,et al. Two isomers of HDTIC isolated from Astragali Radix decrease the expression of p16 in 2BS cells[J]. CHINESE MEDICAL JOURNAL,2008,121(3):231-235.
APA Wang Pei-Chang,Zhang Zong-Yu,Ran, Zhang,&Tong Tan-Jun.(2008).Two isomers of HDTIC isolated from Astragali Radix decrease the expression of p16 in 2BS cells.CHINESE MEDICAL JOURNAL,121(3),231-235.
MLA Wang Pei-Chang,et al."Two isomers of HDTIC isolated from Astragali Radix decrease the expression of p16 in 2BS cells".CHINESE MEDICAL JOURNAL 121.3(2008):231-235.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Wang Pei-Chang]的文章
[Zhang Zong-Yu]的文章
[Ran, Zhang]的文章
百度学术
百度学术中相似的文章
[Wang Pei-Chang]的文章
[Zhang Zong-Yu]的文章
[Ran, Zhang]的文章
必应学术
必应学术中相似的文章
[Wang Pei-Chang]的文章
[Zhang Zong-Yu]的文章
[Ran, Zhang]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。