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学科主题: 公共卫生
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Two isomers of HDTIC isolated from Astragali Radix decrease the expression of p16 in 2BS cells
作者: Wang Pei-Chang1; Zhang Zong-Yu1,2; Ran, Zhang; Tong Tan-Jun2
关键词: HDTIC ; Astragali Radix ; replicative senescence ; fibroblast ; gene expression
刊名: CHINESE MEDICAL JOURNAL
发表日期: 2008-02-05
卷: 121, 期:3, 页:231-235
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, General & Internal
研究领域[WOS]: General & Internal Medicine
关键词[WOS]: HUMAN-DIPLOID FIBROBLASTS ; REPLICATIVE SENESCENCE ; CELLULAR SENESCENCE ; P16(INK4A) EXPRESSION ; INHIBITORS ; DELAY ; REPERFUSION ; INVOLVEMENT ; P21(WAF1) ; APOPTOSIS
英文摘要:

Background Astragali Radix, the root of Astragalus membranceus (Fish) Bunge Var. mongholicus (Bge), is a crude drug considered as one of the effective traditional Chinese anti-ageing material. The two isomers of 4-hydroxy-5-hydroxymethyl-[1,3]dioxolan-2,6′-spirane-5′,6′,7′,8′-tetrahydro-indolizine-3′-carbaldehyde (HDTIC), HDTIC-1 and HDTIC-2, were first extracted from the herb in 2002. We demonstrated previously that 0.1 mu mol/L HDTIC-1 or 1.0 mu mol/L HDTIC-2 strongly delay replicative senescence of human fetal lung diploid fibroblasts (2BS). In this study, we chose them to investigate their effects on the expression of senescence-associated genes to explore the mechanism of how HDTIC delays replicative senescence.

Methods The effects of HDTIC-1 and HDTIC-2 on the expression of p16 and p21 were observed in vitro by RT-PCR and Western blot. The anti-oxidative activities of the compounds were also observed by phenotype alteration after treatment with antioxidants. Results There was an obvious expression of p16 in the control senescent cells. However, in the 2BS cells, after 56 population doublings (PDs) grown from PD28 in 0.1 mu mol/L HDTIC-1 or 1.0 mu mol/L HDTIC-2, there was a weak mRNA expression of p16 and no protein expression of p16 was observed. The expression level of p2l increased with cell ageing. Moreover, there was no difference between the expression level of p21 in the control cells and that in the same PD cells cultured with HDTIC compounds. The results also showed that 2BS cells exposed to 100 mu mol/L H(2)O(2) for 5 minutes return to their non-senescent phenotype and continue to be confluent after incubating the damaged cells with HDTIC-1 (1.0 mu mol/L) or HDTIC-2 (10 mu mol/L) for 1 hour.

Conclusions Expression of p16 by 2BS cells was strongly inhibited by HDTIC compounds, which could contribute to their delayed replicative senescence by the way of p16(INK4a)/Rb/MAPK. The anti-oxidative activities of HDTIC-1 and HDTIC-2, described in this study for the first time, might be indirectly related to their inhibition of p16 expression.

语种: 英语
WOS记录号: WOS:000253116600009
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/124331
Appears in Collections:北京大学医药卫生分析中心_期刊论文

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作者单位: 1.Capital Med Univ, Xuanwu Hosp, Dept Lab Med, Beijing 100053, Peoples R China
2.Peking Univ, Med & Hlth Ctr, Ageing Researching Ctr, Beijing 100083, Peoples R China

Recommended Citation:
Wang Pei-Chang,Zhang Zong-Yu,Ran, Zhang,et al. Two isomers of HDTIC isolated from Astragali Radix decrease the expression of p16 in 2BS cells[J]. CHINESE MEDICAL JOURNAL,2008,121(3):231-235.
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