IR@PKUHSC  > 北京大学第三临床医学院  > 成形外科
学科主题临床医学
Basic and Clinical Evidence of an Alternative Method to Produce Vivo Nanofat
Bi, Hong-Sen1; Zhang, Chen1; Niel, Fang-Fei1; Pan, Bo-Lin1; Xiao, E.2
通讯作者Xiao, E.(2)
关键词Adipose Tissue Cell Therapy Mesenchymal Stromal Cells Rejuvenation
刊名CHINESE MEDICAL JOURNAL
2018-03-05
DOI10.4103/0366-6999.226074
131期:5页:588-+
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Medicine, General & Internal
研究领域[WOS]General & Internal Medicine
关键词[WOS]FAT GRAFTING EVIDENCE ; EMULSIFICATION TECHNIQUES ; CELLS ; TISSUE
英文摘要

Background: Fat grafting technologies are popularly used in plastic and reconstructive surgery. Due to its size limitation, it is hard to directly inject untreated fat tissue into the dermal layer. Nanofat, which was introduced by Tonnard, solves this problem by mechanically emulsifying fat tissue. However, the viability of the cells was greatly destroyed. In this study, we reported a new method by "gently" digesting the fat tissue to produce viable adipocytes, progenitors, and stromal stem cells using collagenase I digestion and centrifugation. This was named "Vivo nanofat".

Methods: Human liposuction aspirates were obtained from five healthy female donors with mean age of 28.7 5.6 years. Colony-forming assay, flow cytometry analysis, and adipogenic and osteogenic induction of the adherent cells from the Vivo nanofat were used to characterize the adipose mesenchymal stem cells (MSCs). To investigate in vivo survival, we respectively injected Vivo nanofat and nanofat subcutaneously to the back of 8-week-old male BALB/c nude mice. Samples were harvested 2 days, 2 weeks, and 4 weeks postinjection for measurement, hematoxylin and eosin staining, and immunostaining.

Results: Our results showed that the Vivo nanofat contained a large number of colony-forming cells. These cells expressed MSC markers and had multi-differentiative potential. In vivo transplantation showed that the Vivo nanofat had lower resorption ratio than that of nanofat. The size of the transplanted nanofat was obviously smaller than that of Vivo nanofat 4 weeks postinjection (0.50 +/- 0.17 cm vs. 0.81 +/- 0.07 cm, t = -5783, P = 0.01).

Conclusion: Vivo nanofat may serve as a cell fraction injectable through a fine needle; this could be used for cosmetic applications.

语种英语
WOS记录号WOS:000426089500013
通讯作者邮箱xiaoe@bjmu.edu.cn
第一作者单位Peking Univ, Hosp 3, Dept Plast Surg, Beijing 100191, Peoples R China
通讯作者单位Peking Univ, Dept Oral & Maxillofacial Surg, Sch & Hosp Stomatol, Natl Engn Lab Digital & Mat Technol Stomatol,Beij, Beijing 100081, Peoples R China
ISSN0366-6999
引用统计
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/136250
专题北京大学第三临床医学院_成形外科
北京大学口腔医学院_外科
北京大学临床肿瘤学院_淋巴肿瘤内科
作者单位1.Peking Univ, Hosp 3, Dept Plast Surg, Beijing 100191, Peoples R China;
2.Peking Univ, Dept Oral & Maxillofacial Surg, Sch & Hosp Stomatol, Natl Engn Lab Digital & Mat Technol Stomatol,Beij, Beijing 100081, Peoples R China
推荐引用方式
GB/T 7714
Bi, Hong-Sen,Zhang, Chen,Niel, Fang-Fei,et al. Basic and Clinical Evidence of an Alternative Method to Produce Vivo Nanofat[J]. CHINESE MEDICAL JOURNAL,2018,131(5):588-+.
APA Bi, Hong-Sen,Zhang, Chen,Niel, Fang-Fei,Pan, Bo-Lin,&Xiao, E..(2018).Basic and Clinical Evidence of an Alternative Method to Produce Vivo Nanofat.CHINESE MEDICAL JOURNAL,131(5),588-+.
MLA Bi, Hong-Sen,et al."Basic and Clinical Evidence of an Alternative Method to Produce Vivo Nanofat".CHINESE MEDICAL JOURNAL 131.5(2018):588-+.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Bi, Hong-Sen]的文章
[Zhang, Chen]的文章
[Niel, Fang-Fei]的文章
百度学术
百度学术中相似的文章
[Bi, Hong-Sen]的文章
[Zhang, Chen]的文章
[Niel, Fang-Fei]的文章
必应学术
必应学术中相似的文章
[Bi, Hong-Sen]的文章
[Zhang, Chen]的文章
[Niel, Fang-Fei]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。