IR@PKUHSC  > 北京大学基础医学院  > 药理学系
学科主题基础医学
Manganese promotes intracellular accumulation of AQP2 via modulating F-actin polymerization and reduces urinary concentration in mice
Lei, Lei1,2,3; Huang, Ming1,2,3; Su, Limin1,2,3; Xie, Dongping2,3; Mamuya, Fahmy A.2,3,4; Ham, Onju2,3,4; Tsuji, Kenji2,3,4; Paunescu, Teodor G.2,3,4; Yang, Baoxue1; Lu, Hua A. Jenny2,3,4
通讯作者Lu, Hua A. Jenny(2,3,4)
关键词aquaporin-2 endocytosis manganese urine concentration
刊名AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
2018-02-01
DOI10.1152/ajprenal.00391.2017
314期:2页:F306-F316
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Physiology ; Urology & Nephrology
研究领域[WOS]Physiology ; Urology & Nephrology
关键词[WOS]AQUAPORIN-2 WATER CHANNEL ; RENAL EPITHELIAL-CELLS ; NEPHROGENIC DIABETES-INSIPIDUS ; PUTATIVE PHOSPHORYLATION SITES ; KIDNEY COLLECTING DUCT ; PLASMA-MEMBRANE ; APICAL MEMBRANE ; PC12 CELLS ; SURFACE EXPRESSION ; DIVALENT-CATIONS
英文摘要

Aquaporin-2 (AQP2) is a water channel protein expressed in principal cells (PCs) of the kidney collecting ducts (CDs) and plays a critical role in mediating water reabsorption and wine concentration. AQP2 undergoes both regulated trafficking mediated by vasopressin (VP) and constitutive recycling, which is independent of VP. For both pathways, actin cytoskeletal dynamics is a key determinant of AQP2 trafficking. We report here that manganese chloride (MnCl2) is a novel and potent regulator of AQP2 trafficking in cultured cells and in the kidney. MnCl2 treatment promoted internalization and intracellular accumulation of AQP2. The effect of MnCl2 on the intracellular accumulation of AQP2 was associated with activation of RhoA and actin polymerization without modification of AQP2 phosphorylation. Although the level of total and phosphorylated AQP2 did not change, MnCl2 treatment impeded VP-induced phosphorylation of AQP2 at its serine-256,-264, and-269 residues and dephosphorylation at serine 261. In addition, MtsCI, significantly promoted F-actin polymerization along with downregulation of RhoA activity and prevented VP-induced membrane accumulation of AQP2. Finally, MnCl2 treatment in mice resulted in significant polyuria and reduced urinary concentration, likely due to intracellular relocation of AQP2 in the PCs of kidney CDs. More importantly, the reduced urinary concentration caused by MtsCI, treatment in animals was not corrected by VP. In summary, our study identified a novel effect of MnCl2 on AQP2 trafficking through modifying RhoA activity and actin polymerization and uncovered its potent impact on water diuresis its vivo.

语种英语
WOS记录号WOS:000426108400017
通讯作者邮箱lu.hua@mgh.harvard.edu
第一作者单位Peking Univ, Sch Basic Med Sci, Dept Pharmacol, Beijing, Peoples R China ; Massachusetts Gen Hosp, Ctr Syst Biol, Program Membrane Biol, Boston, MA USA ; Massachusetts Gen Hosp, Dept Med, Div Nephrol, Boston, MA USA
通讯作者单位Harvard Med Sch, Boston, MA USA ; Massachusetts Gen Hosp, Ctr Syst Biol, Program Membrane Biol, Boston, MA USA ; Massachusetts Gen Hosp, Dept Med, Div Nephrol, Boston, MA USA
ISSN1931-857X
引用统计
被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/136270
专题北京大学基础医学院_药理学系
北京大学临床肿瘤学院_肝胆胰外二科
作者单位1.Harvard Med Sch, Boston, MA USA;
2.Peking Univ, Sch Basic Med Sci, Dept Pharmacol, Beijing, Peoples R China;
3.Massachusetts Gen Hosp, Ctr Syst Biol, Program Membrane Biol, Boston, MA USA;
4.Massachusetts Gen Hosp, Dept Med, Div Nephrol, Boston, MA USA
推荐引用方式
GB/T 7714
Lei, Lei,Huang, Ming,Su, Limin,et al. Manganese promotes intracellular accumulation of AQP2 via modulating F-actin polymerization and reduces urinary concentration in mice[J]. AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY,2018,314(2):F306-F316.
APA Lei, Lei.,Huang, Ming.,Su, Limin.,Xie, Dongping.,Mamuya, Fahmy A..,...&Lu, Hua A. Jenny.(2018).Manganese promotes intracellular accumulation of AQP2 via modulating F-actin polymerization and reduces urinary concentration in mice.AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY,314(2),F306-F316.
MLA Lei, Lei,et al."Manganese promotes intracellular accumulation of AQP2 via modulating F-actin polymerization and reduces urinary concentration in mice".AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY 314.2(2018):F306-F316.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Lei, Lei]的文章
[Huang, Ming]的文章
[Su, Limin]的文章
百度学术
百度学术中相似的文章
[Lei, Lei]的文章
[Huang, Ming]的文章
[Su, Limin]的文章
必应学术
必应学术中相似的文章
[Lei, Lei]的文章
[Huang, Ming]的文章
[Su, Limin]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。