IR@PKUHSC  > 北京大学第九临床医学院
学科主题临床医学
miR-24-3p Regulates Progression of Gastric Mucosal Lesions and Suppresses Proliferation and Invasiveness of N87 Via Peroxiredoxin 6
Li, Qian1,2; Wang, Nina2,3,4; Wei, Hong2,3; Li, Chao2,3; Wu, Jing1; Yang, Guibin2,3
通讯作者Wu, Jing(1) ; Yang, Guibin(2,3)
关键词miR-24 Prdx-6 H. pylori Gastric cancer
刊名DIGESTIVE DISEASES AND SCIENCES
2016-12-01
DOI10.1007/s10620-016-4309-9
61期:12页:3486-3497
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Gastroenterology & Hepatology
研究领域[WOS]Gastroenterology & Hepatology
关键词[WOS]CANCER ; MICRORNAS ; DISEASE ; MIRNA
英文摘要

MicroRNAs, targeting mRNAs of cancer-associated genes, are often aberrantly expressed in human gastric cancer (GC).

We have examined the possible role and mechanisms of miRNA regulation of Prdx-6 in the development and progression of H. pylori-related gastric mucosal lesions.

First, miR-24-3p was predicted to target Prdx-6, and this negative regulation was validated by luciferase reporter analyses, Western blot, and quantitative RT-PCR. Next, immunohistochemistry and in situ hybridization were performed to detect the Prdx-6 and miR-24-3p expression in tissue microarrays of gastric mucosal lesions. Finally, the miR-24-3p function in GC cell line N87 was examined by MTT, Annexin V-FITC, PI, transwell migration, and Matrigel invasion assays.

In our study, Prdx-6 expression was negatively regulated by miR-24-3p expression and miR-24-3p interacted with the 3′-untranslated region of Prdx-6 to down-regulate its expression level. In addition, miR-24-3p expression gradually decreased in human gastric specimens from chronic superficial gastritis (CSG) to dysplasia and was upregulated in GC tissues compared with adjacent normal tissues. Contrary to this, Prdx-6 expression showed inverse tendency in the same tissue. More so, expression of miR-24-3p was reduced in samples with H. pylori infection, especially in CSG. Moreover, miR-24-3p was associated with GC lymph nodes and liver metastasis. Gain- or loss-of-function experiments showed that miR-24-3p significantly inhibited N87 cell growth, migration, and invasion and promoted apoptosis, while Prdx-6 reversed these miR-24-3p effects.

miR-24-3p was identified as a regulator of development and progression of H. pylori-related gastric mucosal lesions.

语种英语
WOS记录号WOS:000388227400018
通讯作者邮箱wujing36@163.com ; ygb@medmail.com.cn
第一作者单位Peking Univ, Sch Clin Med 9, Dept Gastroenterol, Beijing, Peoples R China ; Peking Univ, Aerosp Sch Clin Med, Dept Gastroenterol, Beijing 100039, Peoples R China
通讯作者单位Aerosp Ctr Hosp, Beijing, Peoples R China ; Peking Univ, Sch Clin Med 9, Dept Gastroenterol, Beijing, Peoples R China ; Peking Univ, Aerosp Sch Clin Med, Dept Gastroenterol, Beijing 100039, Peoples R China
ISSN0163-2116
引用统计
被引频次:5[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/139575
专题北京大学第九临床医学院
北京大学第一临床医学院_肾脏内科
作者单位1.Aerosp Ctr Hosp, Beijing, Peoples R China;
2.Peking Univ, Sch Clin Med 9, Dept Gastroenterol, Beijing, Peoples R China;
3.Peking Univ, Aerosp Sch Clin Med, Dept Gastroenterol, Beijing 100039, Peoples R China;
4.Peoples Hosp Feixian, Linyi Shi, Shandong, Peoples R China
推荐引用方式
GB/T 7714
Li, Qian,Wang, Nina,Wei, Hong,et al. miR-24-3p Regulates Progression of Gastric Mucosal Lesions and Suppresses Proliferation and Invasiveness of N87 Via Peroxiredoxin 6[J]. DIGESTIVE DISEASES AND SCIENCES,2016,61(12):3486-3497.
APA Li, Qian,Wang, Nina,Wei, Hong,Li, Chao,Wu, Jing,&Yang, Guibin.(2016).miR-24-3p Regulates Progression of Gastric Mucosal Lesions and Suppresses Proliferation and Invasiveness of N87 Via Peroxiredoxin 6.DIGESTIVE DISEASES AND SCIENCES,61(12),3486-3497.
MLA Li, Qian,et al."miR-24-3p Regulates Progression of Gastric Mucosal Lesions and Suppresses Proliferation and Invasiveness of N87 Via Peroxiredoxin 6".DIGESTIVE DISEASES AND SCIENCES 61.12(2016):3486-3497.
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