IR@PKUHSC  > 北京大学第九临床医学院
学科主题临床医学
A rat model of sustained hypobaric hypoxia-induced pulmonary hypertension
Zhao, Sheng1,2; Xue, Xinying2; Gao, Peiliang2; Deng, Hui2; Yao, Xiaoyan2; Zhang, Jingyuan2; Gao, Nana3; Cui, Lei3; Wang, Yong2; Pan, Lei2; Yang, Shuixiang1
通讯作者Wang, Yong(2) ; Pan, Lei(2) ; Yang, Shuixiang(1)
关键词High-altitude pulmonary hypertension hypobaric hypoxia-induced pulmonary hypertension rat model
刊名INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE
2017
10期:2页:3366-3372
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Medicine, Research & Experimental
研究领域[WOS]Research & Experimental Medicine
关键词[WOS]ARTERIAL-HYPERTENSION ; VENOUS OCCLUSION ; VASOCONSTRICTION ; ASSOCIATION ; MECHANISMS
英文摘要

Objectives: High-altitude pulmonary hypertension (HAPH) is a worldwide public health issue in mountainous areas, while the underlying mechanism of it is still unclear. This study aims to establish a rat model of hypobaric hypoxia-induced pulmonary hypertension through sustained exposure to a simulated high altitude atmospheric environment in plain area. Methods: Twelve healthy male SD rats were randomly and equally divided into a model group and a control group. The model group was housed in an automatic adjusting hypobaric hypoxia chamber for 4 weeks, and the control group was housed under normobaric normoxic condition in the same room. The mean pulmonary arterial pressure (mPAP), right ventricular pressure (RVSP), the right ventricle (RV) weight, left ventricular (LV) weight, interventricular septum (S) weight, right ventricular hypertrophy index (RVHI), hematoxylin-eosinstaining, elastic fibers staining, ratio of the thickness of vascular wall to its outer diameter (MT%), ratio of the cross-sectional area of the middle vascular wall to the total vascular cross-sectional area (MA%), alpha-SMA positive ratio were detected to evaluate the pulmonary hypertension. Results: As compared to that in control group, rats in model group showed significant increases on mPAP (36.39 +/- 4.26 mmHg versus 16.27 +/- 6.99 mmHg, P<0.001), RVSP (45.29 +/- 6.09 mmHg versus 28.12 +/- 4.67mmHg, P<0.001), RVHI (0.44 +/- 0.08 versus 0.18 +/- 0.05, P<0.001), MT% (44.50 +/- 8.96 versus 19.50 +/- 4.88, P<0.001), MA% (64.00 +/- 6.66 versus 40.45 +/- 7.08, P<0.001), alpha-SMA positive% (57.00 +/- 6.87 versus 30.50 +/- 6.83, P<0.001). Conclusion: The rat model of hypobaric hypoxia-induced pulmonary hypertension has been successfully established by automatic adjusting hypobaric hypoxia chamber. Sustained exposure to a low oxygen environment at a simulate-altitude of 5,000 meter for 4 weeks have caused the pathological remodeling of pulmonary vascular walls and pulmonary hypertension, and further led to a series of pathological changes, including right ventricular hypertrophy. This model is easy to be replicated with good reproducibility and can be widely used in further studies.

语种英语
WOS记录号WOS:000396518500182
通讯作者邮箱wangyong_7096@163.com ; leipan2010@163.com ; sxyang68@126.com
第一作者单位Peking Univ, Sch Clin Med 9, Dept Cardiol, 10 Tieyi Rd, Beijing 10038, Peoples R China ; Capital Med Univ, Beijing Shijitan Hosp, Dept Special Med Treatment Resp Dis, 10 Tieyi Rd, Beijing 10038, Peoples R China
通讯作者单位Peking Univ, Sch Clin Med 9, Dept Cardiol, 10 Tieyi Rd, Beijing 10038, Peoples R China ; Capital Med Univ, Beijing Shijitan Hosp, Dept Special Med Treatment Resp Dis, 10 Tieyi Rd, Beijing 10038, Peoples R China
ISSN1940-5901
引用统计
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/139684
专题北京大学第九临床医学院
作者单位1.Peking Univ, Sch Clin Med 9, Dept Cardiol, 10 Tieyi Rd, Beijing 10038, Peoples R China;
2.Capital Med Univ, Beijing Shijitan Hosp, Dept Special Med Treatment Resp Dis, 10 Tieyi Rd, Beijing 10038, Peoples R China;
3.Capital Med Univ, Beijing Shijitan Hosp, Cent Lab, Beijing, Peoples R China
推荐引用方式
GB/T 7714
Zhao, Sheng,Xue, Xinying,Gao, Peiliang,et al. A rat model of sustained hypobaric hypoxia-induced pulmonary hypertension[J]. INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE,2017,10(2):3366-3372.
APA Zhao, Sheng.,Xue, Xinying.,Gao, Peiliang.,Deng, Hui.,Yao, Xiaoyan.,...&Yang, Shuixiang.(2017).A rat model of sustained hypobaric hypoxia-induced pulmonary hypertension.INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE,10(2),3366-3372.
MLA Zhao, Sheng,et al."A rat model of sustained hypobaric hypoxia-induced pulmonary hypertension".INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE 10.2(2017):3366-3372.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Zhao, Sheng]的文章
[Xue, Xinying]的文章
[Gao, Peiliang]的文章
百度学术
百度学术中相似的文章
[Zhao, Sheng]的文章
[Xue, Xinying]的文章
[Gao, Peiliang]的文章
必应学术
必应学术中相似的文章
[Zhao, Sheng]的文章
[Xue, Xinying]的文章
[Gao, Peiliang]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。