IR@PKUHSC  > 北京大学基础医学院  > 中西医结合学系
A DNA nanorobot functions as a cancer therapeutic in response to a molecular trigger in vivo
Li, Suping1,2; Jiang, Qiao1; Liu, Shaoli1,2; Zhang, Yinlong1,3; Tian, Yanhua1,4; Song, Chen1; Wang, Jing1; Zou, Yiguo1; Anderson, Gregory J.5; Han, Jing-Yan6; Chang, Yung7,8; Liu, Yan7,8; Zhang, Chen9; Chen, Liang10; Zhou, Guangbiao9; Nie, Guangjun1,2; Yan, Hao7,8; Ding, Baoquan1,2; Zhao, Yuliang1,2; Nie, Guangjun(1,2); Yan, Hao(7,8); Ding, Baoquan(1,2); Zhao, Yuliang(1,2)
Source PublicationNATURE BIOTECHNOLOGY
2018-03-01
ISSN1087-0156
Volume36Issue:3Pages:258-+
Indexed BySCI
Abstract

Nanoscale robots have potential as intelligent drug delivery systems that respond to molecular triggers(1-4). Using DNA origami we constructed an autonomous DNA robot programmed to transport payloads and present them specifically in tumors. Our nanorobot is functionalized on the outside with a DNA aptamer that binds nucleolin, a protein specifically expressed on tumor-associated endothelial cells(5), and the blood coagulation protease thrombin within its inner cavity. The nucleolin-targeting aptamer serves both as a targeting domain and as a molecular trigger for the mechanical opening of the DNA nanorobot. The thrombin inside is thus exposed and activates coagulation at the tumor site. Using tumor-bearing mouse models, we demonstrate that intravenously injected DNA nanorobots deliver thrombin specifically to tumor-associated blood vessels and induce intravascular thrombosis, resulting in tumor necrosis and inhibition of tumor growth. The nanorobot proved safe and immunologically inert in mice and Bama miniature pigs. Our data show that DNA nanorobots represent a promising strategy for precise drug delivery in cancer therapy.

Subject Area基础医学
SubtypeArticle
DOI10.1038/nbt.4071
WOS HeadingsScience & Technology
Language英语
WOS Research AreaBiotechnology & Applied Microbiology ; Biotechnology & Applied Microbiology
WOS SubjectBiotechnology & Applied Microbiology
WOS KeywordTUMOR VASCULATURE ; DRUG-DELIVERY ; TISSUE FACTOR ; ORIGAMI ; NUCLEOLIN ; CELL ; NANOTECHNOLOGY ; THROMBOSIS ; NANOSCALE ; PROTEINS
WOS IDWOS:000426698700021
Correspondent Emailniegj@nanoctr.cn ; hao.yan@asu.edu ; dingbq@nanoctr.cn ; zhaoyl@nanoctr.cn
Citation statistics
Cited Times:380[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.bjmu.edu.cn/handle/400002259/140474
Collection北京大学基础医学院_中西医结合学系
北京大学基础医学院
北京大学第二临床医学院_泌尿外科
北京大学临床肿瘤学院_淋巴肿瘤内科
北京大学精神卫生研究所_精神科
Corresponding AuthorNie, Guangjun(1,2); Yan, Hao(7,8); Ding, Baoquan(1,2); Zhao, Yuliang(1,2)
Affiliation1.Natl Ctr Nanosci & Technol, CAS Ctr Excellence Nanosci, CAS Key Lab Nanosyst & Hierarch Fabricat, CAS Key Lab Biomed Effects Nanomat & Nanosafety, Beijing, Peoples R China;
2.Univ Chinese Acad Sci, Beijing, Peoples R China;
3.Jilin Univ, Coll Pharmaceut Sci, Changchun, Jilin, Peoples R China;
4.Peking Univ, Acad Adv Interdisciplinary Studies, Peking Tsinghua Ctr Life Sci, Beijing, Peoples R China;
5.QIMR Berghofer Med Res Inst, Brisbane, Qld, Australia;
6.Peking Univ, Sch Basic Med Sci, Dept Integrat Chinese & Western Med, Beijing, Peoples R China;
7.Arizona State Univ, Ctr Mol Design & Biomimet, Sch Mol Sci, Tempe, AZ USA;
8.Arizona State Univ, Sch Life Sci, Ctr Immunotherapy Vaccines & Virotherapy, Biodesign Inst, Tempe, AZ USA;
9.Chinese Acad Sci, Inst Zool, Beijing, Peoples R China;
10.Jinan Univ, Coll Life Sci & Technol, Inst Life & Hlth Engn, Guangzhou, Guangdong, Peoples R China
Recommended Citation
GB/T 7714
Li, Suping,Jiang, Qiao,Liu, Shaoli,et al. A DNA nanorobot functions as a cancer therapeutic in response to a molecular trigger in vivo[J]. NATURE BIOTECHNOLOGY,2018,36(3):258-+.
APA Li, Suping.,Jiang, Qiao.,Liu, Shaoli.,Zhang, Yinlong.,Tian, Yanhua.,...&Zhao, Yuliang.(2018).A DNA nanorobot functions as a cancer therapeutic in response to a molecular trigger in vivo.NATURE BIOTECHNOLOGY,36(3),258-+.
MLA Li, Suping,et al."A DNA nanorobot functions as a cancer therapeutic in response to a molecular trigger in vivo".NATURE BIOTECHNOLOGY 36.3(2018):258-+.
Files in This Item:
File Name/Size DocType Version Access License
A DNA nanorobot func(3450KB)期刊论文出版稿开放获取CC BY-NC-SAApplication Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Li, Suping]'s Articles
[Jiang, Qiao]'s Articles
[Liu, Shaoli]'s Articles
Baidu academic
Similar articles in Baidu academic
[Li, Suping]'s Articles
[Jiang, Qiao]'s Articles
[Liu, Shaoli]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Li, Suping]'s Articles
[Jiang, Qiao]'s Articles
[Liu, Shaoli]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.