IR@PKUHSC  > 北京大学第二临床医学院  > 妇产科
学科主题临床医学
IGHG1 promotes motility likely through epithelial-mesenchymal transition in ovarian cancer
Qian, Jingfeng1; Ji, Fangxing2; Ye, Xue1; Cheng, Hongyan1; Ma, Ruiqiong1; Chang, Xiaohong1; Shou, Chengchao2; Cui, Heng1
通讯作者Cui, Heng(1)
关键词IGHG1 ovarian cancer EMT invasion metastasis
刊名CHINESE JOURNAL OF CANCER RESEARCH
2018-04-01
DOI10.21147/j.issn.1000-9604.2018.02.11
30期:2页:282-290
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology
研究领域[WOS]Oncology
关键词[WOS]LYMPH-NODE METASTASIS ; RESISTANT OVARIAN ; CARCINOMA ; CELLS ; STATISTICS ; MECHANISMS ; THERAPY ; CHINA
英文摘要

Objective: Ovarian cancer (OC) is one of the leading causes of death for female cancer patients. COC166-9 is an OC-specific monoclonal antibody and we have identified immunoglobulin.-1 heavy chain constant region (IGHG1) as its antigen. We explore the function of IGHG1 in proliferation, apoptosis and motility of OC cells further in this research.

Methods: IGHG1 expression in OC specimens was detected through immunohistochemistry. Real-time quantitative polymerase chain reaction (RT-qPCR) or western blotting assay was used to test IGHG1 expression in OC cells. Viability of OC cells was tested by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Flow cytometry or western blotting assay was used to detect cell cycle and apoptosis. Cellular motility was analyzed by using transwell assay and the markers of epithelial-mesenchymal transition (EMT) were tested through immunoblots.

Results: Although it exerts negligible effect on the viability and apoptosis of OC cells, IGHG1 could promote migration and invasion of malignant cells in vitro. Mechanistically, IGHG1 increases the expression of N-cadherin and Vimentin while decreases E-cadherin expression. Additionally, IGHG1 expression in OC specimens is higher relative to the paired normal counterparts. Further analysis demonstrates that the increased IGHG1 expression correlates positively with the lymph node metastasis of OC.

Conclusions: IGHG1 promotes the motility of OC cells likely through executing the EMT program. Increased IGHG1 expression in OC specimens is associated with the lymph node metastasis.

语种英语
WOS记录号WOS:000433656400011
通讯作者邮箱cuiheng@pkuph.edu.cn
第一作者单位Peking Univ, Peoples Hosp, Dept Obstet & Gynecol, Ctr Gynecol Oncol, 11 Xizhimen South St, Beijing 100044, Peoples R China
通讯作者单位Peking Univ, Peoples Hosp, Dept Obstet & Gynecol, Ctr Gynecol Oncol, 11 Xizhimen South St, Beijing 100044, Peoples R China
ISSN1000-9604
引用统计
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/141870
专题北京大学第二临床医学院_妇产科
北京大学第二临床医学院_妇科肿瘤中心
北京大学临床肿瘤学院_生化与分子生物学研究室
作者单位1.Peking Univ, Peoples Hosp, Dept Obstet & Gynecol, Ctr Gynecol Oncol, 11 Xizhimen South St, Beijing 100044, Peoples R China;
2.Peking Univ, Canc Hosp & Inst, Key Lab Carcinogenesis & Translat Res, Minist Educ Beijing,Dept Biochem & Mol Biol, Beijing 100142, Peoples R China
推荐引用方式
GB/T 7714
Qian, Jingfeng,Ji, Fangxing,Ye, Xue,et al. IGHG1 promotes motility likely through epithelial-mesenchymal transition in ovarian cancer[J]. CHINESE JOURNAL OF CANCER RESEARCH,2018,30(2):282-290.
APA Qian, Jingfeng.,Ji, Fangxing.,Ye, Xue.,Cheng, Hongyan.,Ma, Ruiqiong.,...&Cui, Heng.(2018).IGHG1 promotes motility likely through epithelial-mesenchymal transition in ovarian cancer.CHINESE JOURNAL OF CANCER RESEARCH,30(2),282-290.
MLA Qian, Jingfeng,et al."IGHG1 promotes motility likely through epithelial-mesenchymal transition in ovarian cancer".CHINESE JOURNAL OF CANCER RESEARCH 30.2(2018):282-290.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Qian, Jingfeng]的文章
[Ji, Fangxing]的文章
[Ye, Xue]的文章
百度学术
百度学术中相似的文章
[Qian, Jingfeng]的文章
[Ji, Fangxing]的文章
[Ye, Xue]的文章
必应学术
必应学术中相似的文章
[Qian, Jingfeng]的文章
[Ji, Fangxing]的文章
[Ye, Xue]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。