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学科主题临床医学
Cardiac Hypertrophy is Positively Regulated by MicroRNA-24 in Rats
Gao, Juan1,2,3,4,5; Zhu, Min1,2,3,4,5; Liu, Rui-Feng1,2,3,4,5; Zhang, Jian-Shu1,2,3,4,5; Xu, Ming1,2,3,4,5
关键词Cardiac Hypertrophy Cell Cycle Microrna Microrna-24
刊名CHINESE MEDICAL JOURNAL
2018-06-05
DOI10.4103/0366-6999.232793
131期:11页:1333-1341
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Medicine, General & Internal
研究领域[WOS]General & Internal Medicine
关键词[WOS]Cell-cycle ; Heart-failure ; Expression ; Inhibition ; Suppression ; Transition ; P27(Kip1) ; Prevents ; Fibrosis ; Therapy
英文摘要

Background: Micro RNA-24 (miR-24) plays art important role in heart failure by reducing the efficiency of myocardial excitation-contraction coupling. Prolonged cardiac hypertrophy may lead to heart failure, but little is known about the role of miR-24 in cardiac hypertrophy. This study aimed to preliminarily investigate the function of miR-24 and its mechanisms in cardiac hypertrophy.

Methods: Twelve Sprague-Dawley rats with a body weight of 50 +/- 5 g were recruited and randomly divided into two groups: a transverse aortic constriction (TAC) group and a sham surgery group. Hypertrophy index was measured and calculated by echocardiography and hematoxylin and eosin staining. TargetScans algorithm-based prediction was used to search for the targets of miR-24, which was subsequently confirmed by a real-time polymerase chain reaction and luciferase assay. Immunofluorescence labeling was used to measure the cell surface area, and H-3-leucine incorporation was used to detect the synthesis of total protein in neonatal rat cardiac myocytes (NRCMs) with the overexpression of miR-24. In addition, flow cytometry was performed to observe the alteration in the cell cycle. Statistical analysis was carried out with GraphPad Prism v5.0 and SPSS 19.0. A two-sided P < 0.05 was considered as the threshold for significance.

Results: The expression of miR-24 was abnormally increased in TAC rat cardiac tissue (t = -2.938, P < 0.05), TargetScans algorithm-based prediction demonstrated that CDKN1B (p27, Kipl), a cell cycle regulator, was a putative target of miR-24, and was confirmed by luciferase assay. The expression of p27 was decreased in TAC rat cardiac tissue (t = 2.896, P < 0.05). The overexpression of miR-24 in NRCMs led to Ute decreased expression of p27 (t = 4.400, P < 0.01), and decreased G0/G1 arrest in cell cycle and cardiomyocyte hypertrophy.

Conclusion: MiR-24 promotes cardiac hypertrophy partly by affecting the cell cycle through down-regulation of p27 expression.

语种英语
WOS记录号WOS:000432883400013
通讯作者邮箱xuminghi@bjmu.edu.cn
ISSN0366-6999
引用统计
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/141932
专题北京大学第三临床医学院_心血管内科
北京大学口腔医学院_特诊科
作者单位1.Peking Univ, Hosp 3, Dept Cardiol, Beijing 100191, Peoples R China;
2.Peking Univ, Hosp 3, Inst Vasc Med, Beijing 100191, Peoples R China;
3.Minist Hlth, Key Lab Cardiovasc Mol Biol & Regulatory Peptides, Beijing 100191, Peoples R China;
4.Minist Educ, Key Lab Mol Cardiovasc Sci, Beijing 100191, Peoples R China;
5.Beijing Key Lab Cardiovasc Receptors Res, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Gao, Juan,Zhu, Min,Liu, Rui-Feng,et al. Cardiac Hypertrophy is Positively Regulated by MicroRNA-24 in Rats[J]. CHINESE MEDICAL JOURNAL,2018,131(11):1333-1341.
APA Gao, Juan,Zhu, Min,Liu, Rui-Feng,Zhang, Jian-Shu,&Xu, Ming.(2018).Cardiac Hypertrophy is Positively Regulated by MicroRNA-24 in Rats.CHINESE MEDICAL JOURNAL,131(11),1333-1341.
MLA Gao, Juan,et al."Cardiac Hypertrophy is Positively Regulated by MicroRNA-24 in Rats".CHINESE MEDICAL JOURNAL 131.11(2018):1333-1341.
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