|Human trophoblast cell during first trimester after IVF-ET differs from natural conceived pregnancy in development and function|
|Yang, Rui1,2; Liu, Ying-ying1,2; Zhao, Liang1,2; Wang, Ying1,2; Li, Rong1,2; Liu, Ping1,2; Ma, Cai-hong1,2; Chen, Xin-na1,2; Qiao, Jie1,2|
|关键词||Cytotrophoblast cell Syncytiotrophoblast cell In vitro fertilization and embryo transfer Natural pregnancy Proteomic analysis|
|刊名||HISTOLOGY AND HISTOPATHOLOGY|
|WOS标题词||Science & Technology|
|类目[WOS]||Cell Biology ; Pathology|
|研究领域[WOS]||Cell Biology ; Pathology|
|关键词[WOS]||IN-VITRO-FERTILIZATION ; PROTEOMIC ANALYSIS ; ALPHA-FETOPROTEIN ; OXIDATIVE STRESS ; HUMAN PLACENTA ; APOPTOSIS|
Objective. To explore the differences of the trophoblast cell function in first trimester between natural pregnancy and pregnancy after IVF-ET therapy.
Methods. 102 cases with twin to singleton fetal reduction after IVF-ET treatment from July 2010 to August 2013 in Peking University Third Hospital were involved in analysis, and eight specimens were obtained from this group. 10 natural-pregnancy cases undergoing artificial abortion with unwanted pregnancy were recruited as control. Semi-quantitative immunohistochemical method was used to detect the expression of EGFR, Bcl-2, tubulin-alpha, metallothionein and AFP in villi in both groups.
Results. Of the 102 cases, 14 cases (13.73%) were aborted. Preterm birth occurred in seven cases (7.86%). Low birth weight occurred in three patients (3.37%), and extremely low birth weight occurred in four cases (4.49%). The expression of EGFR, tubulin-alpha, Bcl-2, and metallothionein in the IVF-ET group was significantly lower than that in the control group (P<0.05). However, AFP expression was significantly higher in IVF-ET group than in control group (P<0.05). In IVF-ET group, the miscarriage case had weaker EGFR, tubulin-alpha, and metallothionein expression than full-term pregnancy; the early preterm labor case had weaker Bcl-2, tubulin-alpha, and metallothionein expression; and velamentous cord insertion case had weaker tubulin-alpha expression.
Conclusions. The trophoblast cell function of IVF-ET group in first trimester is different from control group in proliferation, invasion, apoptosis and vascular development, and optimal pregnancy outcome depends on the self-healing balance of trophoblast cells.
|作者单位||1.Peking Univ, Reprod Med Ctr, Dept Obstet & Gynecol, Hosp 3,Key Lab Assisted Reprod,Minist Educ, Beijing, Peoples R China;|
2.Beijing Key Lab Reprod Endocrinol & Assisted Repr, Beijing, Peoples R China
|Yang, Rui,Liu, Ying-ying,Zhao, Liang,et al. Human trophoblast cell during first trimester after IVF-ET differs from natural conceived pregnancy in development and function[J]. HISTOLOGY AND HISTOPATHOLOGY,2017,32(3):243-251.|
|APA||Yang, Rui.,Liu, Ying-ying.,Zhao, Liang.,Wang, Ying.,Li, Rong.,...&Qiao, Jie.(2017).Human trophoblast cell during first trimester after IVF-ET differs from natural conceived pregnancy in development and function.HISTOLOGY AND HISTOPATHOLOGY,32(3),243-251.|
|MLA||Yang, Rui,et al."Human trophoblast cell during first trimester after IVF-ET differs from natural conceived pregnancy in development and function".HISTOLOGY AND HISTOPATHOLOGY 32.3(2017):243-251.|