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学科主题临床医学
Identification of miR-18a-5p as an oncogene and prognostic biomarker in RCC
Zhou, Liang1,2,4; Li, Zuwei1,3,4; Pan, Xiang1,4,5; Lai, Yulin1,2,5; Quan, Jing1,4,5; Zhao, Liwen1,4,5; Xu, Jinling1; Xu, Weijie1; Guan, Xin1; Li, Hang1; Yang, Shangqi1; Gui, Yaoting4; Lai, Yongqing1,4
通讯作者Lai, Yongqing(1,4)
关键词MicroRNAs miR-18a-5p renal cell carcinoma oncogene prognosis biomarker
刊名AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH
2018
10期:6页:1874-1886
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology ; Medicine, Research & Experimental
研究领域[WOS]Oncology ; Research & Experimental Medicine
关键词[WOS]RENAL-CELL CARCINOMA ; HEPATOCELLULAR-CARCINOMA ; CIRCULATING MIR-18A ; CANCER ; EXPRESSION ; PROLIFERATION ; MICRORNA-18A ; MIRNAS ; PROGRESSION ; BIOGENESIS
英文摘要

Background: RCC is a malignant tumor that originates from renal tubular epithelial cells, accounting for nearly 90% of renal malignancies and 3% of adult malignancies. It was reported that more than 30-40% of patients with early localized RCC still have recurrence and metastasis after receiving radical surgery. miRNAs are an endogenous non-coding small RNAs that play an important role in the regulation of tumor cell proliferation, differentiation and apoptosis. Methods: In our study, RT-qPCR, CCK-8 assay, wound scratch assay, transwell assay and flow cytometry assay were designed to identify the expression and functions of miR-18a-5p in RCC. Moreover, we collected the survival data from The Cancer Genome Atlas to predict and clarify the prognostic functions of miR-18a-5p in RCC. The correlation between miR-18a-5p expression and clinicopathological variables or overall survival was analyzed by 42 formalin-fixed paraffin-embedded (FFPE) renal cancer samples. Results: The expression of miR-18a-5p in RCC tissues and cell lines was elevated. Further researches suggested that upregulation of miR-18a-5p had a positive effect on RCC cell proliferation, migration, invasion and inhibition of apoptosis, while down-regulation of miR-18a-5p neutralized the effect. In addition, Data of TCGA and prognostic analysis of FFPE RCC samples revealed that high miR-18a-5p expression patients had significantly poorer survival. Conclusions: These results demonstrated that miR-18a-5p functioned as an oncogene and prognostic biomarker in RCC.

语种英语
WOS记录号WOS:000437458800027
通讯作者邮箱yqlord@outlook.com
第一作者单位Peking Univ, Shenzhen Hosp, Dept Urol, 1120 Lianhua Rd, Shenzhen 518036, Guangdong, Peoples R China ; Guangzhou Med Univ, Dept Urol, Guangzhou 511436, Guangdong, Peoples R China ; Peking Univ, Shenzhen Hosp, Guangdong & Shenzhen Key Lab Male Reprod Med & Ge, Inst Urol,Shenzhen PKU HKUST Med Ctr, Shenzhen 518036, Guangdong, Peoples R China
通讯作者单位Peking Univ, Shenzhen Hosp, Dept Urol, 1120 Lianhua Rd, Shenzhen 518036, Guangdong, Peoples R China ; Peking Univ, Shenzhen Hosp, Guangdong & Shenzhen Key Lab Male Reprod Med & Ge, Inst Urol,Shenzhen PKU HKUST Med Ctr, Shenzhen 518036, Guangdong, Peoples R China
ISSN1943-8141
引用统计
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/142217
专题北京大学深圳医院_泌尿外科
北京大学第一临床医学院_皮肤性病科
北京大学第三临床医学院_皮肤科
作者单位1.Peking Univ, Shenzhen Hosp, Dept Urol, 1120 Lianhua Rd, Shenzhen 518036, Guangdong, Peoples R China;
2.Guangzhou Med Univ, Dept Urol, Guangzhou 511436, Guangdong, Peoples R China;
3.Shantou Univ, Med Coll, Dept Urol, Shantou 515041, Guangdong, Peoples R China;
4.Peking Univ, Shenzhen Hosp, Guangdong & Shenzhen Key Lab Male Reprod Med & Ge, Inst Urol,Shenzhen PKU HKUST Med Ctr, Shenzhen 518036, Guangdong, Peoples R China;
5.Anhui Med Univ, Dept Urol, Hefei 230032, Anhui, Peoples R China
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GB/T 7714
Zhou, Liang,Li, Zuwei,Pan, Xiang,et al. Identification of miR-18a-5p as an oncogene and prognostic biomarker in RCC[J]. AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH,2018,10(6):1874-1886.
APA Zhou, Liang.,Li, Zuwei.,Pan, Xiang.,Lai, Yulin.,Quan, Jing.,...&Lai, Yongqing.(2018).Identification of miR-18a-5p as an oncogene and prognostic biomarker in RCC.AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH,10(6),1874-1886.
MLA Zhou, Liang,et al."Identification of miR-18a-5p as an oncogene and prognostic biomarker in RCC".AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH 10.6(2018):1874-1886.
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