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Controllable fabrication of bio-bar codes for dendritically amplified sensing of human T-lymphotropic viruses
Wang, Li-juan1; Ren, Ming1; Liang, Li2; Zhang, Chun-yang1
通讯作者Zhang, Chun-yang(1)
关键词statistical manifold phi-family information geometry exponential arcs mixture arcs
刊名CHEMICAL SCIENCE
2018-06-14
DOI10.1039/c8sc01641k
9期:22页:4942-4949
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Multidisciplinary
研究领域[WOS]Chemistry
关键词[WOS]TERMINAL DEOXYNUCLEOTIDYL TRANSFERASE ; ROLLING CIRCLE AMPLIFICATION ; CASCADE SIGNAL AMPLIFICATION ; CHAIN-REACTION AMPLIFICATION ; DNA DETECTION ; ULTRASENSITIVE DETECTION ; LABEL-FREE ; INDUCED CHEMILUMINESCENCE ; FLUORESCENCE DETECTION ; SENSITIVE DETECTION
英文摘要

Human T-lymphotropic virus type II (HTLV-II) is an important type-C retrovirus, closely related to a variety of human diseases. Here, we demonstrate for the first time the controllable fabrication of bio-bar codes for dendritically amplified sensing of low-abundant HTLV-II DNA by the integration of terminal deoxynucleotidyl transferase (TdT)-catalyzed template-free polymerization extension with bio-bar-code amplification (BCA). HTLV-II DNA hybridizes with magnetic microparticle (MMP)-modified capture probe 1, forming a stable DNA duplex with a protruding 3′-hydroxylated sequence which may function as a primer to initiate the TdT-catalyzed first-step polymerization extension for the generation of a poly-thymidine (T) sequence. The resultant poly-T products may hybridize with poly-adenine (A) capture probe 2, inducing the self-assembly of multiple capture probe 2-/reporter probe-functionalized Au nanoparticles (AuNPs) onto the MMP. Subsequently, the reporter probes may act as the primers to initiate the TdT-catalyzed second-step polymerization extension, producing large numbers of G-rich DNAzymes for the generation of an enhanced chemiluminescence signal. Taking advantage of the efficient polymerization extension reaction catalyzed by TdT, the high amplification efficiency of BCA, and the intrinsically high sensitivity of G-rich DNAzyme-driven chemiluminescence, this method exhibits ultrahigh sensitivity with a limit of detection of as low as 0.50 aM and a large dynamic range of 9 orders of magnitude from 1 aM to 1 nM. Moreover, this method can be applied for the discrimination of a single-base mismatch and the measurement of HTLV-II DNA in both human serum and human T-lymphocytic leukemia cells, holding great potential in biomedical research and clinical diagnosis.

语种英语
WOS记录号WOS:000434693300003
通讯作者邮箱cyzhang@sdnu.edu.cn
第一作者单位Shandong Normal Univ, Shandong Prov Key Lab Clean Prod Fine Chem, Collaborat Innovat Ctr Functionalized Probes Chem, Minist Educ,Coll Chem Chem Engn & Mat Sci,Key Lab, Jinan 250014, Shandong, Peoples R China
通讯作者单位Shandong Normal Univ, Shandong Prov Key Lab Clean Prod Fine Chem, Collaborat Innovat Ctr Functionalized Probes Chem, Minist Educ,Coll Chem Chem Engn & Mat Sci,Key Lab, Jinan 250014, Shandong, Peoples R China
ISSN2041-6520
引用统计
被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/142231
专题北京大学第三临床医学院
北京大学第三临床医学院_肿瘤治疗中心
作者单位1.Shandong Normal Univ, Shandong Prov Key Lab Clean Prod Fine Chem, Collaborat Innovat Ctr Functionalized Probes Chem, Minist Educ,Coll Chem Chem Engn & Mat Sci,Key Lab, Jinan 250014, Shandong, Peoples R China;
2.Peking Univ, Hosp 3, Dept Tumor Chemotherapy & Radiat Sickness, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Wang, Li-juan,Ren, Ming,Liang, Li,et al. Controllable fabrication of bio-bar codes for dendritically amplified sensing of human T-lymphotropic viruses[J]. CHEMICAL SCIENCE,2018,9(22):4942-4949.
APA Wang, Li-juan,Ren, Ming,Liang, Li,&Zhang, Chun-yang.(2018).Controllable fabrication of bio-bar codes for dendritically amplified sensing of human T-lymphotropic viruses.CHEMICAL SCIENCE,9(22),4942-4949.
MLA Wang, Li-juan,et al."Controllable fabrication of bio-bar codes for dendritically amplified sensing of human T-lymphotropic viruses".CHEMICAL SCIENCE 9.22(2018):4942-4949.
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