IR@PKUHSC  > 北京大学临床肿瘤学院  > 胃肠肿瘤中心
学科主题临床医学
CRISPR/Cas9 genome editing technology significantly accelerated herpes simplex virus research
Wang, Dong1,2; Wang, Xian-Wang1,2; Peng, Xiao-Chun1,2; Xiang, Ying1,2; Song, Shi-Bao1,2; Wang, Ying-Ying1,2; Chen, Lin3; Xin, Victoria W.4; Lyu, Yan-Ning5; Ji, Jiafu6; Ma, Zhao-Wu1,2; Li, Cheng-Bin7; Xin, Hong-Wu1,2
通讯作者Ma, Zhao-Wu(1,2) ; Li, Cheng-Bin(7) ; Xin, Hong-Wu(1,2)
刊名CANCER GENE THERAPY
2018-06-01
DOI10.1038/s41417-018-0016-3
25期:5页:93-105
收录类别SCI
文章类型Review
WOS标题词Science & Technology
类目[WOS]Biotechnology & Applied Microbiology ; Oncology ; Genetics & Heredity ; Medicine, Research & Experimental
研究领域[WOS]Biotechnology & Applied Microbiology ; Oncology ; Genetics & Heredity ; Research & Experimental Medicine
关键词[WOS]ZINC-FINGER NUCLEASES ; DOUBLE-STRAND BREAKS ; PHASE-I TRIAL ; DNA CLEAVAGE ; HUMAN-CELLS ; MAMMALIAN-CELLS ; GENE-THERAPY ; TARGET DNA ; CRISPR-CAS9 ; CAS9
英文摘要

Herpes simplex viruses (HSVs) are important pathogens and ideal for gene therapy due to its large genome size. Previous researches on HSVs were hampered because the technology to construct recombinant HSVs were based on DNA homology-dependent repair (HDR) and plaque assay, which are inefficient, laborious, and time-consuming. Fortunately, clustered regularly interspaced short palindromic repeat/CRISPR-associated protein 9 (CRISPR/Cas9) recently provided the possibility to precisely, efficiently, and rapidly edit genomes and indeed is successfully being used in HSVs. Importantly, CRISPR/Cas9 technology increased HSV HDR efficiency exponentially by a 10,000-1,000,000 times when making recombinant HSVs, and its combination with flow cytometric technology made HSV recombination practically automatic. These may have a significant impact on virus and gene therapy researches. This review will summarize the latest development and molecular mechanisms of CRISPR/Cas9 genome editing technology and its recent application in HSVs.

语种英语
WOS记录号WOS:000437202000001
通讯作者邮箱zhaowu823@126.com ; hongwu_xin@126.com ; hongwu_xin@126.com
第一作者单位Yangtze Univ, Clin Med Sch 2, Jingzhou 434023, Hubei, Peoples R China ; Yangtze Univ, Hlth Sci Ctr, Ctr Oncol, Jingzhou 434023, Hubei, Peoples R China
通讯作者单位Yangtze Univ, Clin Med Sch 2, Jingzhou 434023, Hubei, Peoples R China ; Yangtze Univ, Hlth Sci Ctr, Ctr Oncol, Jingzhou 434023, Hubei, Peoples R China ; Yangtze Univ, Clin Med Sch 2, Jingzhou Cent Hosp, Dept Lab Med, Jingzhou 434023, Hubei, Peoples R China
ISSN0929-1903
引用统计
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/142268
专题北京大学临床肿瘤学院_胃肠肿瘤中心
北京大学临床肿瘤学院_胃肠肿瘤中心一病区
作者单位1.Montgomery Blair High Sch, Silver Spring, MD 20901 USA;
2.Yangtze Univ, Clin Med Sch 2, Jingzhou 434023, Hubei, Peoples R China;
3.Yangtze Univ, Hlth Sci Ctr, Ctr Oncol, Jingzhou 434023, Hubei, Peoples R China;
4.Changsha Med Univ, Sch Basic Med, Dept Biochem & Mol Biol, Changsha 410219, Hunan, Peoples R China;
5.Beijing Ctr Dis Prevent & Control, Inst Infect Dis & Endem Dis Prevent & Control, Beijing 100013, Peoples R China;
6.Peking Univ, Canc Hosp & Inst, Key Lab Carcinogenesis & Translat Res, Dept Gastrointestinal Surg,Minist Educ, Beijing 100142, Peoples R China;
7.Yangtze Univ, Clin Med Sch 2, Jingzhou Cent Hosp, Dept Lab Med, Jingzhou 434023, Hubei, Peoples R China
推荐引用方式
GB/T 7714
Wang, Dong,Wang, Xian-Wang,Peng, Xiao-Chun,et al. CRISPR/Cas9 genome editing technology significantly accelerated herpes simplex virus research[J]. CANCER GENE THERAPY,2018,25(5):93-105.
APA Wang, Dong.,Wang, Xian-Wang.,Peng, Xiao-Chun.,Xiang, Ying.,Song, Shi-Bao.,...&Xin, Hong-Wu.(2018).CRISPR/Cas9 genome editing technology significantly accelerated herpes simplex virus research.CANCER GENE THERAPY,25(5),93-105.
MLA Wang, Dong,et al."CRISPR/Cas9 genome editing technology significantly accelerated herpes simplex virus research".CANCER GENE THERAPY 25.5(2018):93-105.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Wang, Dong]的文章
[Wang, Xian-Wang]的文章
[Peng, Xiao-Chun]的文章
百度学术
百度学术中相似的文章
[Wang, Dong]的文章
[Wang, Xian-Wang]的文章
[Peng, Xiao-Chun]的文章
必应学术
必应学术中相似的文章
[Wang, Dong]的文章
[Wang, Xian-Wang]的文章
[Peng, Xiao-Chun]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。