学科主题临床医学
Shugan Liangxue Decoction Down-Regulates Estrogen Receptor alpha Expression in Breast Cancer Cells
Zhou Ning1,2; Han Shu-yan1; Chen Yan-zhi1; Zhou Fei1; Zheng Wen-xian1; Li Ping-ping1
通讯作者Li Ping-ping(1)
关键词breast cancer estrogen receptor Shugan Liangxue Decoction estrogen receptor inhibitor
刊名CHINESE JOURNAL OF INTEGRATIVE MEDICINE
2018-07-01
DOI10.1007/s11655-015-2123-4
24期:7页:518-524
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Integrative & Complementary Medicine
研究领域[WOS]Integrative & Complementary Medicine
关键词[WOS]STEROID SULFATASE ; XUE DECOCTION ; AROMATASE ; PROLIFERATION ; WOMEN ; MCF-7 ; MICE
英文摘要

To observe the effect of Shugan Liangxue Decoction (e′e,eaceeEuroae-(1), SGLXD) on estrogen receptor alpha (ER alpha) in human breast cancer cells.

The effect of SGLXD (0.85-5.10 mg/mL) on the proliferation of breast cancer cells were evaluated by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay. The nuclear ER alpha protein levels in MCF-7, T47D and ZR-75-1 cells which treated by SGLXD for 24 h were examined by western blot and immunofluorescence assay. MCF-7 and MDA-MB-231 cells were treated by 17 beta-estradiol (E2) with or without SGLXD, for 24 h, and the E2 targeted genes c-myc and bcl-2 protein product was evaluated by western blot.

SGLXD showed dose-dependent inhibition on the proliferation of MCF-7, T47D and ZR-75-1 cells, but did not inhibit the proliferation of MDA-MB-231 cells. Furthermore, the promotive effect on cell growth induced by E2 was also significantly inhibited by SGLXD treatment. With the treatment of 1.70, 3.40, 5.10 mg/mL SGLXD, the nuclear ER alpha protein level was reduced to 88.1%, 70.4% and 60.9% in MCF-7 cells, and was decreased to 43.0%, 38.4% and 5.9% in ZR-75-1 cells as compared with the control group. In T47D cells, the nuclear ER alpha protein was down-regulated to 51.3% and 4.3% by 3.40 and 5.10 mg/mL SGLXD treatment. The down-regulative effect of SGLXD on nuclear ER alpha was confirmed by immunofluorescence assay. SGLXD decreased the protein product of c-myc and bcl-2.

SGLXD may exhibit selective inhibition effect on the proliferation of ER positive breast cancer cells. SGLXD reduced the nuclear ER alpha expression and the protein product of E2 target gene c-myc and bcl-2.

语种英语
WOS记录号WOS:000439344100006
通讯作者邮箱lppma123@126.com
第一作者单位Peking Univ, Key Lab Carcinogenesis & Translat Res, Minist Educ, Dept Integrat Chinese & Western Med,Canc Hosp Ins, Beijing 100142, Peoples R China ; Capital Med Univ, Beijing Ditan Hosp, Dept Oncol, Beijing 100015, Peoples R China
通讯作者单位Peking Univ, Key Lab Carcinogenesis & Translat Res, Minist Educ, Dept Integrat Chinese & Western Med,Canc Hosp Ins, Beijing 100142, Peoples R China
ISSN1672-0415
引用统计
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/142632
专题北京大学临床肿瘤学院_中西医结合科暨老年肿瘤科
作者单位1.Peking Univ, Key Lab Carcinogenesis & Translat Res, Minist Educ, Dept Integrat Chinese & Western Med,Canc Hosp Ins, Beijing 100142, Peoples R China;
2.Capital Med Univ, Beijing Ditan Hosp, Dept Oncol, Beijing 100015, Peoples R China
推荐引用方式
GB/T 7714
Zhou Ning,Han Shu-yan,Chen Yan-zhi,et al. Shugan Liangxue Decoction Down-Regulates Estrogen Receptor alpha Expression in Breast Cancer Cells[J]. CHINESE JOURNAL OF INTEGRATIVE MEDICINE,2018,24(7):518-524.
APA Zhou Ning,Han Shu-yan,Chen Yan-zhi,Zhou Fei,Zheng Wen-xian,&Li Ping-ping.(2018).Shugan Liangxue Decoction Down-Regulates Estrogen Receptor alpha Expression in Breast Cancer Cells.CHINESE JOURNAL OF INTEGRATIVE MEDICINE,24(7),518-524.
MLA Zhou Ning,et al."Shugan Liangxue Decoction Down-Regulates Estrogen Receptor alpha Expression in Breast Cancer Cells".CHINESE JOURNAL OF INTEGRATIVE MEDICINE 24.7(2018):518-524.
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