|Shugan Liangxue Decoction Down-Regulates Estrogen Receptor alpha Expression in Breast Cancer Cells|
|Zhou Ning1,2; Han Shu-yan1; Chen Yan-zhi1; Zhou Fei1; Zheng Wen-xian1; Li Ping-ping1|
|关键词||breast cancer estrogen receptor Shugan Liangxue Decoction estrogen receptor inhibitor|
|刊名||CHINESE JOURNAL OF INTEGRATIVE MEDICINE|
|WOS标题词||Science & Technology|
|类目[WOS]||Integrative & Complementary Medicine|
|研究领域[WOS]||Integrative & Complementary Medicine|
|关键词[WOS]||STEROID SULFATASE ; XUE DECOCTION ; AROMATASE ; PROLIFERATION ; WOMEN ; MCF-7 ; MICE|
To observe the effect of Shugan Liangxue Decoction (e′e,eacee
The effect of SGLXD (0.85-5.10 mg/mL) on the proliferation of breast cancer cells were evaluated by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay. The nuclear ER alpha protein levels in MCF-7, T47D and ZR-75-1 cells which treated by SGLXD for 24 h were examined by western blot and immunofluorescence assay. MCF-7 and MDA-MB-231 cells were treated by 17 beta-estradiol (E2) with or without SGLXD, for 24 h, and the E2 targeted genes c-myc and bcl-2 protein product was evaluated by western blot.
SGLXD showed dose-dependent inhibition on the proliferation of MCF-7, T47D and ZR-75-1 cells, but did not inhibit the proliferation of MDA-MB-231 cells. Furthermore, the promotive effect on cell growth induced by E2 was also significantly inhibited by SGLXD treatment. With the treatment of 1.70, 3.40, 5.10 mg/mL SGLXD, the nuclear ER alpha protein level was reduced to 88.1%, 70.4% and 60.9% in MCF-7 cells, and was decreased to 43.0%, 38.4% and 5.9% in ZR-75-1 cells as compared with the control group. In T47D cells, the nuclear ER alpha protein was down-regulated to 51.3% and 4.3% by 3.40 and 5.10 mg/mL SGLXD treatment. The down-regulative effect of SGLXD on nuclear ER alpha was confirmed by immunofluorescence assay. SGLXD decreased the protein product of c-myc and bcl-2.
SGLXD may exhibit selective inhibition effect on the proliferation of ER positive breast cancer cells. SGLXD reduced the nuclear ER alpha expression and the protein product of E2 target gene c-myc and bcl-2.
|作者单位||1.Peking Univ, Key Lab Carcinogenesis & Translat Res, Minist Educ, Dept Integrat Chinese & Western Med,Canc Hosp Ins, Beijing 100142, Peoples R China;|
2.Capital Med Univ, Beijing Ditan Hosp, Dept Oncol, Beijing 100015, Peoples R China
|Zhou Ning,Han Shu-yan,Chen Yan-zhi,et al. Shugan Liangxue Decoction Down-Regulates Estrogen Receptor alpha Expression in Breast Cancer Cells[J]. CHINESE JOURNAL OF INTEGRATIVE MEDICINE,2018,24(7):518-524.|
|APA||Zhou Ning,Han Shu-yan,Chen Yan-zhi,Zhou Fei,Zheng Wen-xian,&Li Ping-ping.(2018).Shugan Liangxue Decoction Down-Regulates Estrogen Receptor alpha Expression in Breast Cancer Cells.CHINESE JOURNAL OF INTEGRATIVE MEDICINE,24(7),518-524.|
|MLA||Zhou Ning,et al."Shugan Liangxue Decoction Down-Regulates Estrogen Receptor alpha Expression in Breast Cancer Cells".CHINESE JOURNAL OF INTEGRATIVE MEDICINE 24.7(2018):518-524.|