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学科主题临床医学
Proteomics study of serum exosomes from papillary thyroid cancer patients
Luo, Dan1,2; Zhan, Shaohua1,2; Xia, Wenchao1,2; Huang, Liang3; Ge, Wei1,2; Wang, Tianxiao4
通讯作者Ge, Wei(1,2) ; Wang, Tianxiao(4)
关键词papillary thyroid cancer (PTC) lymph node metastasis (LNM) serum-purified exosomes (SPEs) proteomic analysis
刊名ENDOCRINE-RELATED CANCER
2018-10-01
DOI10.1530/ERC-17-0547
25期:10页:879-891
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology ; Endocrinology & Metabolism
研究领域[WOS]Oncology ; Endocrinology & Metabolism
关键词[WOS]LYMPH-NODE METASTASES ; CARCINOMA CELL-LINE ; SRC FAMILY KINASES ; PROSTATE-CANCER ; BREAST-CANCER ; EXPRESSION ; GROWTH ; PROGRESSION ; TRANSITIONS ; SUPPRESSION
英文摘要

Lymph node metastasis (LNM) in papillary thyroid cancer (PTC) is related to increased risk of recurrence and poor prognosis. Tumour exosomes have been shown to be associated with metastasis of cancer cells. Therefore, we aim to identify the characteristics and biological functions of serum exosomes in lymph node metastases of PTC. We compared proteome profiles of serum-purified exosomes (SPEs) from PTC patients with LNM, PTC patients without LNM, and healthy donors, using a combination of liquid chromatography-tandem mass spectroscopy analyses and tandem mass tag label quantitation analysis. We identified 1569 proteins by two or more unique peptides. Compared with the SPEs of PTC patients without LNM, we found 697 differentially expressed proteins in the SPEs of PTC patients with LNM. Our results revealed overexpression of specific proteins with well-established links to cancer cell metastasis, such as SRC, TLN1, ITGB2 and CAPNS1. Consistent with mass spectrum results, we performed Western blot to detect the expression of these proteins in individual sample. Biological pathway analyses showed that integrin signalling was aberrantly activated in the SPEs of PTC patients with LNM compared to those without LNM. Our study reveals that SPEs of PTC patients with lymph node metastases promote BHT101 thyroid cancer cell invasiveness, but have no apparent influence on cell migration. In the serum exosomes of PTC patients with LNM, integrin-associated proteins are obviously upregulated. These proteomic findings will contribute to elucidation of the pathophysiological functions of tumour-derived exosomes.

语种英语
WOS记录号WOS:000441741000006
通讯作者邮箱wei.ge@chem.ox.ac.uk ; tianxiao_w2000@hotmail.com
第一作者单位Chinese Acad Med Sci, Nat Key Lab Med Mol Biol, Beijing, Peoples R China ; Chinese Acad Med Sci, Dept Immunol, Inst Basic Med Sci, Beijing, Peoples R China
通讯作者单位Chinese Acad Med Sci, Nat Key Lab Med Mol Biol, Beijing, Peoples R China ; Chinese Acad Med Sci, Dept Immunol, Inst Basic Med Sci, Beijing, Peoples R China ; Peking Univ, Dept Head & Neck Surg, Key Lab Carcinogenesis & Translat Res, Canc Hosp & Inst, Beijing, Peoples R China
ISSN1351-0088
引用统计
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/142887
专题北京大学临床肿瘤学院_头颈外科
作者单位1.Chinese Acad Med Sci, Nat Key Lab Med Mol Biol, Beijing, Peoples R China;
2.Chinese Acad Med Sci, Dept Immunol, Inst Basic Med Sci, Beijing, Peoples R China;
3.Huazhong Univ Sci & Technol, Tongji Hosp, Dept Blood Transfus, Tongji Med Coll, Wuhan, Hubei, Peoples R China;
4.Peking Univ, Dept Head & Neck Surg, Key Lab Carcinogenesis & Translat Res, Canc Hosp & Inst, Beijing, Peoples R China
推荐引用方式
GB/T 7714
Luo, Dan,Zhan, Shaohua,Xia, Wenchao,et al. Proteomics study of serum exosomes from papillary thyroid cancer patients[J]. ENDOCRINE-RELATED CANCER,2018,25(10):879-891.
APA Luo, Dan,Zhan, Shaohua,Xia, Wenchao,Huang, Liang,Ge, Wei,&Wang, Tianxiao.(2018).Proteomics study of serum exosomes from papillary thyroid cancer patients.ENDOCRINE-RELATED CANCER,25(10),879-891.
MLA Luo, Dan,et al."Proteomics study of serum exosomes from papillary thyroid cancer patients".ENDOCRINE-RELATED CANCER 25.10(2018):879-891.
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