IR@PKUHSC  > 北京大学基础医学院  > 心血管所
学科主题基础医学
Diagnostic utility of fluorogenic peptide-conjugated Au nanoparticle probe corroborated by rabbit model of mild cartilage injury and panel of osteoarthritic patients
Liu, Zhenlong1,2; Hu, Xiaoqing2; Yang, Peng2; Zhang, Jiying2; Zhou, Chunyan1; Ao, Yingfang2
通讯作者Zhou, Chunyan(1) ; Ao, Yingfang(2)
关键词ADAMTS-4 gold nanoparticle probe cartilage injury
刊名AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH
2018
10期:8页:2277-+
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology ; Medicine, Research & Experimental
研究领域[WOS]Oncology ; Research & Experimental Medicine
关键词[WOS]AGGRECANASE-1 ADAMTS-4 ; IN-VITRO ; DEGENERATION ; DEGRADATION ; EXPRESSION ; CLEAVAGE ; REPAIR ; SITES
英文摘要

Using a rabbit model of early-stage osteoarthritis (OA) and a sampling patients with OA, we evaluated the diagnostic utility of a fluorogenic peptide-conjugated gold nanoparticle (AuNP) probe in detecting mild cartilage injury, based on the a disintegrin and metalloproteinase with thrombospondin motifs 4 (ADAMTS-4) enzyme. Synthesis of this fluorescent turn-on probe (or AU-probe) required conjugation of AuNPs with a fluorescein isothiocyanate (FITC)-modified ADAMTS-4-specific peptide (DVQEFRGVTAVIR). Synovial fluid samples were then collected from 48 adult rabbits and 100 patients for comparative testing (ADAMTS-4 ELISA and AU-probe). Rabbit and patient MRI images were also evaluated and scored. Receiver operating characteristic (ROC) curve analysis was applied to various diagnostic methods (MRI, ELISA, AU-probe, and arthroscopy), performing comparisons via logistic regression. In rabbits, the AU-probe proved nonsuperior to MRI T2 mapping and ELISA (fluorescence cutpoint > 864.965 au). In patient groups, logistic regression analysis indicated that combined AU-probe/MRI testing outperformed MRI alone, thus offsetting low MRI sensitivity and low AU-probe specificity for improved detection of mild cartilage injury (sensitivity, 82.5%; specificity, 80.0%). We have consequently confirmed the efficacy of this AU-probe, using ADAMTS-4 activity in synovial fluid to diagnose mild cartilage injury. Combining the AU-probe with conventional MRI assessment proved optimal in this setting.

语种英语
WOS记录号WOS:000443724100005
通讯作者邮箱chunyan-zhou@bjmu.edu.cn ; aoying-fang@163.com
第一作者单位Peking Univ, Minist Educ, Key Lab Mol Cardiovasc Sci, Dept Biochem & Mol Biol,Sch Basic Med Sci, Beijing 100191, Peoples R China ; Peking Univ, Hosp 3, Beijing Key Lab Sports Injuries, Inst Sports Med, Beijing 100191, Peoples R China
通讯作者单位Peking Univ, Minist Educ, Key Lab Mol Cardiovasc Sci, Dept Biochem & Mol Biol,Sch Basic Med Sci, Beijing 100191, Peoples R China ; Peking Univ, Hosp 3, Beijing Key Lab Sports Injuries, Inst Sports Med, Beijing 100191, Peoples R China
ISSN1943-8141
引用统计
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/142955
专题北京大学基础医学院_心血管所
北京大学基础医学院
北京大学第三临床医学院_骨科
作者单位1.Peking Univ, Minist Educ, Key Lab Mol Cardiovasc Sci, Dept Biochem & Mol Biol,Sch Basic Med Sci, Beijing 100191, Peoples R China;
2.Peking Univ, Hosp 3, Beijing Key Lab Sports Injuries, Inst Sports Med, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Liu, Zhenlong,Hu, Xiaoqing,Yang, Peng,et al. Diagnostic utility of fluorogenic peptide-conjugated Au nanoparticle probe corroborated by rabbit model of mild cartilage injury and panel of osteoarthritic patients[J]. AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH,2018,10(8):2277-+.
APA Liu, Zhenlong,Hu, Xiaoqing,Yang, Peng,Zhang, Jiying,Zhou, Chunyan,&Ao, Yingfang.(2018).Diagnostic utility of fluorogenic peptide-conjugated Au nanoparticle probe corroborated by rabbit model of mild cartilage injury and panel of osteoarthritic patients.AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH,10(8),2277-+.
MLA Liu, Zhenlong,et al."Diagnostic utility of fluorogenic peptide-conjugated Au nanoparticle probe corroborated by rabbit model of mild cartilage injury and panel of osteoarthritic patients".AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH 10.8(2018):2277-+.
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