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学科主题临床医学
Protective effects of low tidal volume mechanical ventilation associated with WISP1-TRL4 signaling pathway in a sepsis model of mice
Jiang, Xi1,2; Jin, Shuqing1; Ding, Xibing1; Tong, Yao1; Wang, Lingyu1; Chen, Zhixia1; Li, Quan1,3,4,5
通讯作者Li, Quan(1,3,4,5)
关键词Low tidal volume mechanical ventilation sepsis WISP1 TLR4
刊名INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE
2018
11期:8页:7872-7884
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Medicine, Research & Experimental
研究领域[WOS]Research & Experimental Medicine
关键词[WOS]ACUTE LUNG INJURY ; RESPIRATORY-DISTRESS-SYNDROME ; ABDOMINAL-SURGERY ; HYPERCAPNIC ACIDOSIS ; GENERAL-ANESTHESIA ; CECAL LIGATION ; SEPTIC SHOCK ; INFLAMMATION ; PROTEIN-1 ; HEALTHY
英文摘要

Purpose: Sepsis is an independent risk factor of acute lung injury (ALI). The use of low tidal volume (LTV) is considered to contribute to decreasing ventilation induced lung injury (VILI) demonstrating protective effects. The purpose of our experiment is to study the time-course effect after low tidal volume mechanical ventilation and evaluate whether the protective effects of low tidal volume mechanical ventilation are associated with WISP1 -TRL4 signaling pathway in a sepsis model of mice. Materials and methods: Adult male wild type C57 mice were subject to mechanical ventilation with low tidal volume (6 ml/kg) and breathe rates 200/min. The cecal ligation puncture (CLP) model was applied to induce a polymicrobial abdominal infection. The mice were divided to 4 groups: 1) Control group; 2) LTV group; 3) CLP group; 4) CLP+LTV group. Lung tissues were harvested to detect the Evans blue album (EBA) permeability, wet/dry ratio, histological and morphometric analysis, as well as The WNT1 inducible signaling pathway protein (WISP1) and Toll-like receptor 4 (TLR4) protein and mRNA expression. Serum and bronchoalveolar lavage fluid (BALF) were obtained to analysis proinflammatory cytokines (interleukin (IL)-6 and tumor necrosis factor (TNF)-alpha). Results: A significant increase in the EBA permeability, wet/dry ratio, IL-6 and TNF-alpha protein levels in serum and BALF was seen in CLP-treated mice compared with the control mice. The low tidal volume contributed to the decrease of lung injury from CLP-treated mice. The decrease was the most significant at the 4th hour while receded with time prolonged. The expression of WISP1 and TLR4 protein and mRNA were synergistically down-regulated when LTV mechanical ventilation was treated. Conclusion: After ventilation with LTV for 1 h, 2 h, 4 h, 8 h, 12 h the impairment for the lung of the sepsis mice are gradually improved while aggravate with time prolonged. At the 4th hour, the protective effect of low tidal volume to sepsis-induced lung injury is the most obvious. The latent mechanism that the low tidal volume ventilation contributes to the decrease lung injury in the sepsis-induced ALI model, which is partially associated with WISP1 -TLR4 signaling pathway.

语种英语
WOS记录号WOS:000443533700037
通讯作者邮箱liquantongji@163.com
第一作者单位Tongji Univ, Sch Med, East Hosp, Dept Anesthesiol, Shanghai, Peoples R China ; Peking Univ, Hosp 3, Dept Anesthesiol, Beijing 100191, Peoples R China
通讯作者单位Tongji Univ, Sch Med, East Hosp, Dept Anesthesiol, Shanghai, Peoples R China ; Chinese Acad Med Sci, Canc Hosp, Natl Clin Res Ctr Canc, Dept Anesthesiol,Natl Canc Ctr, Shenzhen 518116, Peoples R China ; Chinese Acad Med Sci, Shenzhen Hosp, Shenzhen 518116, Peoples R China ; Peking Union Med Coll, Shenzhen 518116, Peoples R China
ISSN1940-5901
引用统计
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/142989
专题北京大学第三临床医学院_麻醉科
北京大学口腔医学院_口腔种植科
作者单位1.Tongji Univ, Sch Med, East Hosp, Dept Anesthesiol, Shanghai, Peoples R China;
2.Peking Univ, Hosp 3, Dept Anesthesiol, Beijing 100191, Peoples R China;
3.Chinese Acad Med Sci, Canc Hosp, Natl Clin Res Ctr Canc, Dept Anesthesiol,Natl Canc Ctr, Shenzhen 518116, Peoples R China;
4.Chinese Acad Med Sci, Shenzhen Hosp, Shenzhen 518116, Peoples R China;
5.Peking Union Med Coll, Shenzhen 518116, Peoples R China
推荐引用方式
GB/T 7714
Jiang, Xi,Jin, Shuqing,Ding, Xibing,et al. Protective effects of low tidal volume mechanical ventilation associated with WISP1-TRL4 signaling pathway in a sepsis model of mice[J]. INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE,2018,11(8):7872-7884.
APA Jiang, Xi.,Jin, Shuqing.,Ding, Xibing.,Tong, Yao.,Wang, Lingyu.,...&Li, Quan.(2018).Protective effects of low tidal volume mechanical ventilation associated with WISP1-TRL4 signaling pathway in a sepsis model of mice.INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE,11(8),7872-7884.
MLA Jiang, Xi,et al."Protective effects of low tidal volume mechanical ventilation associated with WISP1-TRL4 signaling pathway in a sepsis model of mice".INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE 11.8(2018):7872-7884.
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