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学科主题临床医学
A growth factor-free culture system underscores the coordination between Wnt and BMP signaling in Lgr5(+) intestinal stem cell maintenance
Li, Yehua1; Liu, Yuan1; Liu, Bofeng2; Wang, Jilian3; Wei, Siting1; Qi, Zhen1; Wang, Shan1; Fu, Wei3; Chen, Ye-Guang1
通讯作者Chen, Ye-Guang(1)
刊名CELL DISCOVERY
2018-09-04
DOI10.1038/s41421-018-0051-0
4
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell Biology
研究领域[WOS]Cell Biology
关键词[WOS]CONDITIONAL KNOCKOUT MICE ; SELF-RENEWAL ; COLON ; GENERATION ; EPITHELIUM ; ORGANOIDS
英文摘要

Lgr5(+) intestinal stem cells (ISCs) drive the fast renewal of intestinal epithelium. Several signaling pathways have been shown to regulate ISC fates. However, it is unclear what are the essential signals to sustain the ISC self-renewal. Here we show that coordination between Wnt and BMP signaling activity is necessary and sufficient to maintain Lgr5(+) ISCs self-renewal. The key function of R-spondinl is to achieve a high activity of Wnt signaling in the organoid culture. Using the GSK3 inhibitor CHIR-99021 and the BMP type I receptor inhibitor LDN-193189, we can maintain Lgr5(+) ISCs without growth factors in vitro. Our results define the basic signaling pathways sustaining Lgr5(+) ISCs and set up a convenient and economical culture system for their in vitro expansion. This work also set up an example for growth factor-free culture of other adult stem cells.

语种英语
WOS记录号WOS:000443456300001
通讯作者邮箱ygchen@tsinghua.edu.cn
第一作者单位Tsinghua Univ, Tsinghua Peking Ctr Life Sci, Sch Life Sci, State Key Lab Membrane Biol, Beijing 100084, Peoples R China
通讯作者单位Tsinghua Univ, Tsinghua Peking Ctr Life Sci, Sch Life Sci, State Key Lab Membrane Biol, Beijing 100084, Peoples R China
ISSN2056-5968
引用统计
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/142991
专题北京大学第三临床医学院_普通外科
作者单位1.Tsinghua Univ, Tsinghua Peking Ctr Life Sci, Sch Life Sci, State Key Lab Membrane Biol, Beijing 100084, Peoples R China;
2.Tsinghua Univ, Tsinghua Peking Ctr Life Sci, Sch Life Sci, Beijing 100084, Peoples R China;
3.Peking Univ, Dept Gen Surg, Hosp 3, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Li, Yehua,Liu, Yuan,Liu, Bofeng,et al. A growth factor-free culture system underscores the coordination between Wnt and BMP signaling in Lgr5(+) intestinal stem cell maintenance[J]. CELL DISCOVERY,2018,4.
APA Li, Yehua.,Liu, Yuan.,Liu, Bofeng.,Wang, Jilian.,Wei, Siting.,...&Chen, Ye-Guang.(2018).A growth factor-free culture system underscores the coordination between Wnt and BMP signaling in Lgr5(+) intestinal stem cell maintenance.CELL DISCOVERY,4.
MLA Li, Yehua,et al."A growth factor-free culture system underscores the coordination between Wnt and BMP signaling in Lgr5(+) intestinal stem cell maintenance".CELL DISCOVERY 4(2018).
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