学科主题临床医学
Marsdenia tenacissima extract induces apoptosis and suppresses autophagy through ERK activation in lung cancer cells
Jiao, Yan-Na1; Wu, Li-Na2; Xue, Dong1; Liu, Xi-Juan2; Tian, Zhi-Hua2; Jiang, Shan-Tong1; Han, Shu-Yan1; Li, Ping-Ping1
通讯作者Han, Shu-Yan(1) ; Li, Ping-Ping(1)
关键词Marsdenia tenacissima extract (MTE) Apoptosis Autophagy ERK activation NSCLC
刊名CANCER CELL INTERNATIONAL
2018-09-27
DOI10.1186/s12935-018-0646-4
18
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology
研究领域[WOS]Oncology
关键词[WOS]IN-VITRO ; DEATH ; PATHWAY ; PROLIFERATION ; INHIBITION ; SENESCENCE ; MECHANISM ; GROWTH ; VIVO
英文摘要

Background: Marsdenia tenacissima is an herb medicine which has been utilized to treat malignant diseases for decades. The M. tenacissima extract (MTE) shows significant anti-proliferation activity against non-small cell lung cancer (NSCLC) cells, but the underlying mechanisms remain unclear. In this study, we explored the potential anti-proliferation mechanisms of MTE in NSCLC cells in relation to apoptosis as well as autophagy, which are two critical forms to control cancer cell survival and death.

Methods: The proliferation of H1975 and A549 cells was evaluated by MTT assay. Cell apoptosis was assessed by Annexin V and PI staining, Caspase 3 expression and activity. Autophagy flux proteins were detected by Western blot with or without autophagy inducer and inhibitor. Endogenous LC3-II puncta and LysoTracker staining were monitored by confocal microscopy. The formation of autophagic vacuoles was measured by acridine orange staining. ERK is a crucial molecule to interplay with cell autophagy and apoptosis. The role of ERK on cell apoptosis and autophagy influenced by MTE was determined in the presence of MEK/ERK inhibitor U0126.

Results: The significant growth inhibition and apoptosis induction were observed in MTE treated NSCLC cells. MTE induced cell apoptosis coexisted with elevated Caspase 3 activity. MTE also impaired autophagic flux by upregulated LC3-II and p62 expression. Autophagy inducer EBSS could not abolish the impaired autophagic flux by MTE, while it was augmented in the presence of autophagy inhibitor Baf A1. The autophagosome-lysosome fusion was blocked by MTE via affecting lysosome function as evidenced by decreased expression of LAMP1 and Cathepsin B. The molecule ERK became hyperactivated after MTE treatment, but the MEK/ERK inhibitor U0126 abrogated autophagy inhibition and apoptosis induction caused by MTE, suggested that ERK signaling pathways partially contributed to cell death caused by MTE.

Conclusion: Our results demonstrate that MTE caused apoptosis induction as well as autophagy inhibition in NSCLC cells. The activated ERK is partially associated with NSCLC apoptotic and autophagic cell death in response to MTE treatment. The present findings reveal new mechanisms for the anti-tumor activity of MTE against NSCLC.

语种英语
WOS记录号WOS:000445819500002
通讯作者邮箱shuyanhan@bjmu.edu.cn ; lppma123@sina.com
第一作者单位Peking Univ, Canc Hosp & Inst, Dept Integrat Chinese & Western Med, Key Lab Carcinogenesis & Translat Res,Minist Educ, 52 Fucheng Rd, Beijing 100142, Peoples R China
通讯作者单位Peking Univ, Canc Hosp & Inst, Dept Integrat Chinese & Western Med, Key Lab Carcinogenesis & Translat Res,Minist Educ, 52 Fucheng Rd, Beijing 100142, Peoples R China
ISSN1475-2867
引用统计
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/145563
专题北京大学临床肿瘤学院_中西医结合科暨老年肿瘤科
北京大学临床肿瘤学院_中心实验室
作者单位1.Peking Univ, Canc Hosp & Inst, Dept Integrat Chinese & Western Med, Key Lab Carcinogenesis & Translat Res,Minist Educ, 52 Fucheng Rd, Beijing 100142, Peoples R China;
2.Peking Univ, Canc Hosp & Inst, Cent Lab, Key Lab Carcinogenesis & Translat Res,Minist Educ, 52 Fucheng Rd, Beijing 100142, Peoples R China
推荐引用方式
GB/T 7714
Jiao, Yan-Na,Wu, Li-Na,Xue, Dong,et al. Marsdenia tenacissima extract induces apoptosis and suppresses autophagy through ERK activation in lung cancer cells[J]. CANCER CELL INTERNATIONAL,2018,18.
APA Jiao, Yan-Na.,Wu, Li-Na.,Xue, Dong.,Liu, Xi-Juan.,Tian, Zhi-Hua.,...&Li, Ping-Ping.(2018).Marsdenia tenacissima extract induces apoptosis and suppresses autophagy through ERK activation in lung cancer cells.CANCER CELL INTERNATIONAL,18.
MLA Jiao, Yan-Na,et al."Marsdenia tenacissima extract induces apoptosis and suppresses autophagy through ERK activation in lung cancer cells".CANCER CELL INTERNATIONAL 18(2018).
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