北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第二临床医学院  > 北京大学血液病研究所  > 期刊论文
学科主题: 血液病
题名:
急性T淋巴细胞白血病异基因造血干细胞移植后WT1基因表达及其与预后关系
其他题名: The relationship between WT1 expression level and prognosis in patients of acute T lymphoblastic leukemia following allogeneic hematopoietic stem cell transplantation
作者: 张博; 赵晓甦; 秦亚溱; 王昱; 闫晨华; 许兰平; 张晓辉; 刘开彦; 黄晓军
关键词: WT1基因 ; 白血病,T淋巴细胞,急性 ; 造血干细胞移植 ; 复发 ; WT1 gene ; Leukemia,T lymphoblastic,acute ; Hematopoietic stem cell transplantation ; Relapse
刊名: 中华血液学杂志
发表日期: 2015
DOI: 10.3760/cma.j.issn.0253-2727.2015.08.003
卷: 36, 期:8, 页:642-646
收录类别: 中国科技核心期刊 ; 中文核心期刊 ; CSCD
文章类型: Journal Article
摘要: 目的 探讨监测WT1基因在急性T淋巴细胞白血病(T-ALL)患者异基因造血干细胞移植(allo-HSCT)后预后的意义.方法 回顾性分析2009年1月至2012年3月在我单位行allo-HSCT,且移植后连续监测WT1基因表达水平的68例T-ALL患者.留取初治,移植前,+30、+60、+90、+180、+270、+360 d骨髓标本,用实时定量聚合酶链反应(RQ-PCR)方法监测WT1基因表达水平,同时经流式细胞术(FCM)监测微小残留病(MRD).结果 ①移植后WT1基因低水平表达与较低复发风险相关;②+60、+90 d WT1基因升高与较高累计复发率相关(P< 0.001、P=0.003),与较低的无病生存率(P=0.004、P=0.006)和总生存率相关(P=0.004、P=0.007);③移植后微小残留病(MRD)阳性是T-ALL移植后复发的独立危险因素;④联合WT1基因和FCM可用于移植后复发的监测.结论 +60、+90 d WT1基因升高与T-ALL预后显著相关,应尽早予以干预,减少复发甚至死亡风险.T-ALL移植后WT1基因表达低于0.6%,复发风险较低;WT1基因表达超过0.6%需密切随访,并结合FCM监测,达到MRD阳性标准需临床干预,降低复发率. Objective To probe monitoring Wilms tumor-1 (WT1) gene expression level in acute T lymphoblastic leukemia (T-ALL) following allogeneic hematopoietic stem cell transplantation (alloHSCT) with prognostic significance.Methods This retrospective study analyzed 68 T-ALL cases from January 2009 to March 2012,that monitoring WT1 gene expression level after allo-HSCT.WT1 expression level was measured with real-time quantitative reverse transcription polymerase chain reaction (RQ-PCR) method at + 30,+ 60,+ 90,+ 180,+ 270,+ 360 days after allo-HSCT,simultaneously monitoring residual leukemia using flow cytometry(FCM).Results Low WT1 gene expression level associated with a low risk of recurrence after allo-HSCT in T-ALL.Increased WT1 gene expression levels at +60 and + 90 days after allo-HSCT associated with higher cumulative incidences of relapse (P<0.001,P=0.003),and low disease-free survival rates (P=0.004,P=0.006),and low overall survival rates (P=0.004,P=0.007).The presence of MRD after allo-HSCT was an independent prognostic factor for relapse in T-ALL.Combining WT1 gene and FCM could be used to monitor recurrence after allo-HSCT.Conclusions Increased WT1 gene expression level at +60 and + 90 days after allo-HSCT significantly associated with worse prognosis,that should be intervened as early as possible to reduce the risk of recurrence or death.WT1 gene expression level that was less than 0.6% associated with lower risk of recurrence.WT1 gene expression more than 0.6% that needed close follow-up,combined with FCM monitoring MRD,which required intervention to reduce the relapse.
语种: 中文
原文出处: 查看原文
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/21293
Appears in Collections:北京大学第二临床医学院_北京大学血液病研究所_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 造血干细胞移植治疗血液病北京市重点实验室, 北京大学人民医院、北京大学血液病研究所,100044
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[张博]'s Articles
[赵晓甦]'s Articles
[秦亚溱]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[张博]‘s Articles
[赵晓甦]‘s Articles
[秦亚溱]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace