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学科主题感染疾病科
HBV转染增强细胞因子上调PD-L表达
其他题名HBV transinfected enhances the PD-L expression of cytokines stimulating
吴学杰1; 陈姬1; 朱曼华1; 洪源1; 钟璐1; 王贵强1
关键词细胞因子类 肝炎病毒 乙型 Pd-l1 Pd-l2 Cytokinins Hepatitis b Virus Pd-l1 Pd-l2
刊名中华实验和临床病毒学杂志
2008
DOI10.3760/cma.j.issn.1003-9279.2008.05.005
22期:5页:333-335
收录类别中国科技核心期刊 ; CSCD
文章类型Journal Article
摘要目的 研究HBV转染的肝细胞系(HepG2.2.15细胞)在细胞因子作用下能否上调PD-L表达.方法 应用肝细胞系(HepG2细胞和HepG2.2.15细胞)为模型,用IL-4、IFN-α、IFN-γ(终质量浓度均为10 ng/ml,刺激时间为12 h)作用于上述细胞系,采用RT-PCR技术检测细胞因子作用前后PD-L表达情况.结果 无论是否转染HBV,IFN-α和IFN-γ均能诱导肝细胞系(HepG2细胞和HepG2.2.15细胞)PD-L1表达;而IL-4不能诱导PD-L1表达,IL-4、IFN-α、IFN-γ均能诱导转染了HBV的HepG2.2.15细胞PD-L2表达,仅IFN-γ能诱导未转染HBV的HepG2细胞PD-L2表达.结论 IFN-α和IFN-γ有较强诱导肝细胞系(HepG2细胞和HepG2.2.15细胞)PD-L1表达上调的作用,HBV在细胞因子诱导HepG2.2.15细胞PD-L2表达中具有促进作用. Objective To investigate whether the PD-L expression in the liver cell lines transinfected with HBV(HepG2.2.15 cells) can be up-regulated after cytokines stimulating. Methods To apply the liver cell lines (HepG2 cells and HepG2.2.15 cells) as a model, the cells were stimulated with IL-4, IFN-α and IFN-γ (final concentration were 10 ng/ml, stimulated for 12 hours) and RT-PCR was carried out to determine the PD-L expression before and after cytokines stimulating. Results Whether or not transinfected with HBV, IFN-α and IFN-γ both can induce the liver cell lines (HepG2 cells and HepG2.2.15 cells) PD-L1 expression while IL-4 can not; IL-4, IFN-α, IFN-γ all can induce the PD-L2 expression in HepG2.2.15 cells which was transinfected with HBV, only IFN-γ can induce the PD-L2 expression in HepG2 cells which was not transinfected with HBV. Conclusion IFN-α, IFN-γ both can induce the PD-L1 expression in HepG2 cells and HepG2.2.15 cells, while it is easy for cytokines to induce the PD-L2 expression in HepG2.2.15 cells which was transinfeeted with HBV, this may provide a potential mechanism of the molecular basis for chronic HBV infection.
语种中文
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文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/41852
Collection北京大学第一临床医学院_感染疾病科
作者单位1.北京大学第一医院感染疾病科,100034
2.辽宁省阜新市传染病医院
Recommended Citation
GB/T 7714
吴学杰,陈姬,朱曼华,等. HBV转染增强细胞因子上调PD-L表达[J]. 中华实验和临床病毒学杂志,2008,22(5):333-335.
APA 吴学杰,陈姬,朱曼华,洪源,钟璐,&王贵强.(2008).HBV转染增强细胞因子上调PD-L表达.中华实验和临床病毒学杂志,22(5),333-335.
MLA 吴学杰,et al."HBV转染增强细胞因子上调PD-L表达".中华实验和临床病毒学杂志 22.5(2008):333-335.
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