北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第一临床医学院  > 放射治疗科  > 期刊论文
学科主题: 放射医学
题名:
冬虫夏草雄激素样作用及对人前列腺癌VCaP细胞放射敏感性影响
其他题名: Androgen-like effects of Cordyceps sinensis and its impact on the radiosensitivity of VCaP prostate cancer cells
作者: 马茗微1; 高献书1; 鱼红亮1; 亓昕1; 孙少倩1; 李晓颖1; 马元元1
关键词: 前列腺癌细胞系 ; 冬虫夏草 ; 放射敏感性 ; 雄激素受体 ; 雄激素样作用 ; Prostate cancer cell line ; Cordyceps sinensis ; Radiosensitivity ; Androgen receptor ; Androgen-like effects
刊名: 中华放射肿瘤学杂志
发表日期: 2015
DOI: 10.3760/cma.j.issn.1004-4221.2015.03.028
卷: 24, 期:3, 页:344-348
收录类别: 中国科技核心期刊 ; 中文核心期刊 ; CSCD
文章类型: Journal Article
摘要: 目的 探讨冬虫夏草雄激素样作用及对人前列腺癌VCaP细胞放射敏感性影响.方法 检测冬虫夏草灌胃后小鼠激素水平及性器官重量指数变化.克隆形成实验方法观察冬虫夏草对VCaP细胞放射敏感性影响.MTS、流式细胞术、裸鼠移植瘤检测冬虫夏草对VCaP细胞(雄激素受体AR阳性)及PC-3细胞(AR阴性)体外及体内增殖能力影响,比较裸鼠血清PSA水平.单因素方差分析或t检验差异.结果 冬虫夏草组裸鼠睾酮水平高于对照组[(8.28±1.94)、(2.08±1.24)ng/ml,P=0.023),前列腺重量指数也增高[(0.53±0.04)、(0.31±0.04) mg/g,P=0.006].放射增敏比(D0值比)为0.80.冬虫夏草处理后VCaP细胞活力高于对照组(1.32±0.07、0.66±0.02,P=0.000),克隆形成能力较对照组增强(57.0%±1.9%、47.0%±0.6%,P=0.005).冬虫夏草组裸鼠VCaP移植瘤有生长加快趋势,血清PSA升高显著[(0.66±0.04)、(0.26±0.06)ng/ml,P=0.000).该浓度冬虫夏草对PC-3细胞无影响.结论 冬虫夏草有雄激素样作用,同时可促进AR阳性的VCaP细胞增殖能力,降低其放射敏感性. Objective To investigate the androgen-like effects of Cordyceps sinensis (CS) and its impact on the radiosensitivity of VCaP prostate cancer cells.Methods The hormone levels and weight index of reproductive organs in mice were determined after gavage with CS.Clonogenic assay was performed to determine the impact of CS on the radiosensitivity of VCaP cells.The 3-(4,5-dimethylthiazol-2-yl)-5 (3-carboxymethonyphenol)-2-(4-sulfophenyl)-2H-tetrazolium assay,flow cytometry,and tumor xenografts in nude mice were performed to determine the effects of CS on the proliferation of VCaP cells (androgen receptor-positive) and PC-3 cells (androgen receptor-negative) in vitro and in vivo.Serum prostate-specific antigen (PSA) levels in nude mice were evaluated.Data were analyzed by one-way analysis of variance or ttest.Results The testosterone level and weight of prostate in mice were significantly higher in the CS group than in the control group ((8.28± 1.94) vs.(2.08± 1.24) ng/ml,P=0.023;(0.53±0.04) vs.(0.31 ± 0.04) mg/g,P =0.006).The radiosensitivity enhancement ratio (ratio of D0 values) was 0.80.The viability of VCaP cells was significantly higher in the CS group than in the control group (1.32 ± 0.07 vs.0.66 ±0.02,P=0.000),and colony forming efficiency was significantly enhanced in the CS group than in the control group (57.0% ± 1.9% vs.47.0% ± 0.6%,P =0.005).VCaP tumor xenografts in nude mice were inclined to grow faster in the CS group than in the control group,and the serum PSA level in the CS group was significantly higher than that in the control group ((0.66 ± 0.04) vs.(0.26 ±0.06) ng/ml,P =0.000).However,CS had no effect on PC-3 cells at the working concentration.Conclusions CS has the androgen-like effects.It may also promote the proliferation and reduce the radiosensitivity of androgen receptor-positive VCaP cells.
语种: 中文
原文出处: 查看原文
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/42185
Appears in Collections:北京大学第一临床医学院_放射治疗科_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.100034,北京大学第一医院放疗科
2.江苏省肿瘤医院放疗科, 南京,210000
3.100034,北京大学第一医院实验动物中心
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[马茗微]'s Articles
[高献书]'s Articles
[鱼红亮]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[马茗微]‘s Articles
[高献书]‘s Articles
[鱼红亮]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace