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IR@PKUHSC  > 北京大学第一临床医学院  > 放射治疗科  > 期刊论文
学科主题: 放射医学
题名:
食管癌原发肿瘤及亚临床灶MMP-2和Survivin基因表达的初步研究
其他题名: Pilot research of the expression of MMP-2 gene and Survivin gene in primary esophageal cancer and subclinical microscopic tumor
作者: 吴凤鹏1; 高献书1; 王雅棣1; 卢付河1; 董稚明1; 乔学英1
关键词: 食管肿瘤 ; 亚临床灶 ; 癌周组织 ; 基因表达 ; Esophageal neoplasms ; Subclinical microscopic tumor ; Cancerous peripheral tissue ; Gene expression
刊名: 中华放射肿瘤学杂志
发表日期: 2008
DOI: 10.3321/j.issn:1004-4221.2008.04.005
卷: 17, 期:4, 页:269-273
收录类别: 中国科技核心期刊 ; 中文核心期刊 ; CSCD
文章类型: Journal Article
摘要: 目的 对食管鳞癌癌周亚临床病灶组织和与之相衔接的分化正常的食管组织中MMP-2基因和Survivin基因的表达情况进行检测,为从分子水平研究食管鳞癌的放疗靶区提供参考.方法 对34例食管鳞癌患者的癌及癌周组织标本进行连续取材并应用免疫组化法检测MMP-2基因和Survivin基因在癌组织和癌周组织的表达情况,测量两基因在癌周食管组织中的阳性表达范围,并对该范围值与肿瘤分期、肿瘤纵向长度的关系进行分析.结果 MMP-2、Survivin基因在癌组织中阳性表达率分别为85%、76%,在阳性表达标本中癌周亚临床病灶的分别为83%、85%,相邻正常食管组织中的分别为79%、85%;亚临床病灶组织中MMP-2、Survivin基因的阳性表达水平明显高于癌周正常食管组织的表达(χ2=6.46,P=0.028;χ2=16.15,P=0.001).食管上、下两端癌阉食管组织中MMP-2基因的阳性表达范围为17.2~70.4 mm和15.0~82.4 mm,其中97%以上患者的表达范围<70 mm;Survivin基因阳性表达范围为3.7~76.4 am和16.1~56.3 mm,其中96%以上患者的表达范围<70 mm;两基因在癌周食管组织中的阳性表达范围随肿瘤分期的进展呈增加趋势.随着肿瘤纵向长度的增加两基因在癌周食管组织中的阳性表达纵向范围呈现增加趋势,其中Survivin基因在癌周食管上下端中的阳性表达范围与肿瘤的纵向长度之间存在正相关性(r=0.566、0.416,P=0.003、0.034).结论 MMP-2、Survivin基因在食管癌周组织中存在一定程度和范围阳性表达,其中96%以上患者两基因阳性表达范围<70 mm且该范围内大小与肿瘤病期进展密切相关,而相邻分化正常食管组织中也存在着阳性表达应给予关注. Objective To examine the expression of MMP-2 gene and Survivin gene in subclinical microscopic tumor and its peripheral normal esophageal tissues,and study the radiation target in molecular level. Methods Esophageal squamous cancer and its peripheral tissue samples of 34 patients were cut into sequential sections.The expression of MMP-2 gene and Survivin gene then examined.The length of the peripheral esophageal tissue,positively expressing the two genes,was measured,and the relation among the experimental date,tumor stage and vertical length of tumor were analyzed. Results For tumor tissue,subclinical microscopic tumor and the peripheral differentiated normal tissue,the positive expression rate of MMP-2 was 85%,83%and 79%,respectively.The positive expression rate of Survivin was 76%,85%and 85%,respectively.The positive expression level of both MMP-2 and Survivin genes in subclinical microscopic tumor was significantly higher than that in the peripheral differentiated normal tissue(χ2=6.46,P=0.028 and χ2=16.15,P=0.001).The length was 17.2-70.4 mm and 15.0-82.4 mm of cancerous peripheral tissue with positive expression of MMP-2 gene upside and downside of the tumor.The length was<70 mm in 97% of the samples.For Survivin gene.the length was 3.7-76.4 mm and 16.1-56.3 mm.and was<70 mm in 96%of the samples.The length of cancerous peripheral esophageal tissue expressing the two genes increased significantly along with tumor stage or tumor length,and there was statistical correlation between the length of tumor and the positive expression ranges of Survivin gene. Conclusions Both MMP-2 gene and Survivin gene are positively expressed in esophageal cancerous peripheral tissue.The range positively expressing the two genes is<70 mm in more than 96%of the samples,and the length is correlated with the tumor stage.More attention should be paid to the peripheral differentiated normal tissue with positive expression of MMP-2 gene and Survivin gene in esophageal squamous carcinoma.
语种: 中文
原文出处: 查看原文
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内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/42250
Appears in Collections:北京大学第一临床医学院_放射治疗科_期刊论文

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作者单位: 1.北京大学第一医院放疗科
2.河北医科大学第四医院放疗科,050000
3.河北医科大学第四医院肿瘤所,050000
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