|摘要||目的 淫羊藿次苷Ⅱ是淫羊藿中提取分离的单体,为了解淫羊藿次苷Ⅱ对阴茎性功能障碍(ED)的疗效,通过体内试验观察其对阴茎海绵体压力和平均动脉血压的影响及作用机制.方法 麻醉下游离大鼠左侧颈总动脉和左侧阴茎海绵体,允别插管与电生理仪连接;游离左侧海绵体神经(CN),采用电生理仪刺激器连接双极电极刺激;检测不同浓度的淫羊藿次苷Ⅱ在刺激CN后对阴茎海绵体压力(ICP)和平均动脉血压(MAP)影响,淫羊藿苷、西地那非、罂粟碱作为对照组.同时观察可溶性环鸟苷酸(sGC)抑制剂H-[1,2,4]噁二唑[4,3.A]喹喔啉(ODQ)、一氧化氮合酶(NOS)抑制剂N~ω硝基-L-精氨酸(LNNA)及一氧化氮生成抑制剂亚甲蓝(methylene blue,MB)对淫羊藿次苷Ⅱ(10~(-4)mol/L)诱发ICP/MAP变化的影响.结果 4组药物均剂量依赖性显著提高ICP(P<0.01),淫羊藿次苷Ⅱ、淫羊藿苷和西地那非对MAP无显著影响(P>0.05),而罂粟碱浓度达10~(-4)mol/L后,即可显著降低MAP(P<0.01).在受试浓度下,LNNA、MB和ODQ可显著抑制淫羊藿次苷Ⅱ诱发的ICP变化(P<0.01),对两地那非诱发的ICP变化无影响(P>0.05).结论 淫羊藿次苷Ⅱ和淫羊藿苷呈剂量依赖性地提高大鼠阴茎勃起功能(ICP),淫羊藿次苷Ⅱ效价强度为淫羊藿苷的2.44倍,对平均动脉血压没有显著影响,其机制可能与增强阴茎海绵体NO-cGMP通路的活性有关.
Objective To realize the effect of Icariside Ⅱ isolated from natural herbal medicine on erectile dysfunction, intracavernosal pressure (ICP) and mean arterial pressure(MAP) including its mechanism was investigated in in vivo study. Methods The rat corpora cavernosa (CC) and left carotid artery were isolated unilaterally, a 26G needle catheter was inserted into the left CC to monitor the intracavernosal pressure (ICP) and administrate drug.Another catheter was placed into the carotid artery to monitor MAP. Rat cavernous nerve (CN) was identified for electric stimulation. Different concentrations of Icariside Ⅱ were administrated intracavernosally, ICP and MAP were recorded during electric stimulation on CN. Icariin, Sildenafil and Papavreine were used as positive controls. The effects of soluble guanylate cyclase inhibitor H-[1,2,4] oxadiazole[4,3,-A] quinoxalin-1-one (ODQ), nitric oxide formation inhibitor methylene blue (MB) and nitric oxide syntheses (NOS) inhibitor N~ω-nitro-L-arginine (LNNA) on Icariside Ⅱ (10~(-4)mol/L) induced ICP MAP changes were also investigated. Results All four kinds of drugs increased the ICP in a dose-depended manner (P<0.01), and Icariside Ⅱ, Icariin, Sildenafil did not significanly influence on MAP (P>0.05).However, Papavreine at the concentration of 10~(-4) mol/L significantly reduced MAP (P<0.01). ODQ, MB and LNNA significantly inhibited ICP induced by Icariside Ⅱ and Icariin (P<0.01), however, the effect of Sildenafil was not significanly influenced by the above three inhibitors. (P>0.05). Conclusion Both of Icariside Ⅱ and Icariin can enhance erectile function(ICP) without infuluence on MAP by specific activation on NO-cGMP signal pathway in CC.|