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学科主题核医学
131I-cRGD环肽对荷黑色素瘤小鼠的靶向治疗作用
其他题名An experimental study on targeted therapy of radioiodinated cyclic cRGD peptide on melanoma bearing mice
崔永刚1; 张春丽1; 王荣福1; 闫平1; 于明明1; 刘红洁1; 付占立1; 郭凤琴1; 赵媛1; 刘萌1; 康磊1; 丁义1
关键词黑色素瘤 肿瘤细胞 培养的 肽类 碘放射性同位素 小鼠 Melanoma Tumor Cells Iodine Radioisotopes Cultured Peptides Mice Cyclic
刊名中华核医学杂志
2009
DOI10.3760/cma.j.issn.0253-9780.2009.02.006
29期:2页:92-95
收录类别中文核心期刊
文章类型Journal Article
摘要目的 用131I标记环精氨酸-甘氨酸-天冬氨酸(cRGD)肽-探讨131I-cRGD对荷黑色素瘤小鼠模型瘤体生长的双重抑制作用.方法 应用氯胺T法对cRGD进行131I标记.建立荷黑色素瘤B16株小鼠模型,将20只荷瘤小鼠按完全随机化法分为4组,每组5只.即实验(131I-cRGD)组、cRGD对照组、131I-甘氨酸-甘氨酸-甘氨酸(cGGG)对照组和空白对照(PBS)组.分别经尾静脉注射相应药物,观察各组倚瘤小鼠肿瘤体积和质量的变化,利用单因素方差分析比较各组之间的差异.结果 131I-cRGD的标记率和放化纯分别达到(89.14±4.57)%及(99.14±0.72)%.治疗第2l天时.各组与PBS组肿瘤体积相比.抑瘤率分别为64.92%(131I-cRGD组)、37.70%(cRGD组)及24.78%(131I-cGGG组);治疗第28天时,处死各组小鼠,其瘤质量(含治疗第2l~28天自然死亡的4只小鼠)分别为131I-cRGD组(6.48±5.19)g、cRGD组(10.81±6.25)g、131I-cGGG组(14.21±5.91)g、PBS组(18.88±7.59)g(F=3.479,P<0.05),131I-cRGD组与PBS组之间差异有统计学意义(t=3.12,P<0.05).各组与PBS组肿瘤质量相比,抑瘤率分别为65.72%(131I-cRGD组)、42.76%(cRGD组)及24.77%(131I-cGGG组).治疗第28天时,各组小鼠净体质量(净体质量=小鼠体质量一瘤质量,含治疗第2l~28天死亡的4只小鼠)分别为(29.12±8.66)g(131I-cRGD组)、(25.89±6.49)g(cRGD组)、(24.29±2.97)g(131I-cGGG组)、(20.92±5.95)g(PBS组).差异尤统计学意义(F=1.444,P>0.05).结论 131I-cRGD能抑制荷黑色素瘤小鼠肿瘤的生长,对黑色素瘤治疗具有潜在的价值. Objective Ectogenic Arg-Gly-Asp(RGD)peptide can bind to integrin α2 β3 receptor on the cell membrane of tumor cells and neovasculature endothelial cells to prevent tumor growth and metas-tasis but was less potent than cRGD.The aim of this study wag to further understand the inhibitory effect of cRGD labeled with 131I in melanoma bearing mice.Methods cRGD peptide was labeled with 131I by Ch-T method under the optimum labeling conditions.Twenty mice were randomly (complete randomization) di-vided into four groups.five for each group.There were experimental group(131I-cRGD),cRGD control group.131l-Gly-Gly-Gly(cGGG)control group and phosphate buffered saline(PBS)control group.All mice were injected through tail vein.Tumor volume, weight and net weight (net weight =weight-the weight of the tumor)were measured for all and compared by one-way ANOVA.Results The labeling effi-ciency and the radiochemical purity of 131I-cRGD peptide were(89.14±4.57)%and(99.14±O.72)%respectively.Compared with the PBS group at 21st day, the tumor inhibitory rates were 64.92%for 131I-cRGD group.37.70% for cRGD group,and 24.78%for 131I-cGGG group respectively.When sacrificed at 28th day,the weight was(6.48±5.19)g for 131I-cRGD group,(10.81±6.25)g for cRGD group, (14.21±5.91)g for 131I-cGGG group,and(18.88±7.59)g for PBS group(F=3.479,P<0.05).Significant difference was observed between 131I-cRGD group and PBS group(t=3.12,P<0.05).Corn- pared with the PBS group at the end of treatment,the tumor inhibitory rates were 65.72%for 131I-cRGD group,42.76% for cRGD group,and 24.77%for 131I-cGGG group respectively and with net weight of (29.12±8.66)g for 131I-cRGD group,(25.89±6.49)g for cRGD group.(24.29 ±2.97)g for".I-cGGG group,and(20.92±5.95)g for PBS group(F=1.444,P>0.05).Conclusion 131I-cRGD could effectively inhibit tumor growth in melanoma bearing mice and might be of potential in treating melano-ma in, the future.
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文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/42995
Collection北京大学第一临床医学院_核医学科
作者单位1.100034北京大学第一医院核医学科
2.北京大学深圳医院影像科
3.北京大学第一医院泌尿外科研究所
Recommended Citation
GB/T 7714
崔永刚,张春丽,王荣福,等. 131I-cRGD环肽对荷黑色素瘤小鼠的靶向治疗作用[J]. 中华核医学杂志,2009,29(2):92-95.
APA 崔永刚.,张春丽.,王荣福.,闫平.,于明明.,...&丁义.(2009).131I-cRGD环肽对荷黑色素瘤小鼠的靶向治疗作用.中华核医学杂志,29(2),92-95.
MLA 崔永刚,et al."131I-cRGD环肽对荷黑色素瘤小鼠的靶向治疗作用".中华核医学杂志 29.2(2009):92-95.
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