北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第一临床医学院  > 核医学科  > 期刊论文
学科主题: 核医学
题名:
碘标记血清淀粉样P成分在动物模型中的体内分布及显像研究
其他题名: The biodistribution and imaging of a novel amyloid probe 131I-serum amyloid P-component in mice model of amyloidosis
作者: 魏海亮; 张春丽; 王荣福; 张建华; 闫平; 康磊; 郭凤琴
关键词: 淀粉样蛋白P成分 ; 碘放射性同位素 ; 同位素标记 ; 淀粉样变 ; 放射性核素显像 ; 小鼠 ; Amyloid P component ; Iodine radioisotopes ; Amyloidosis ; Isotope labeling ; Radionuclide imaging ; Mice
刊名: 中华核医学杂志
发表日期: 2011
DOI: 10.3760/cma.j.issn.0253-9780.2011.06.014
卷: 31, 期:6, 页:410-413
收录类别: 中文核心期刊
文章类型: Journal Article
摘要: 目的 探讨131 I标记的人血清淀粉样P成分(SAP)诊断淀粉样变性的价值.方法 用Iodogen法对SAP标准品进行131I标记,测定标记率与放化纯,观察标记物的稳定性;对实验组小鼠用质量分数10%酪蛋白(Casein)0.5 ml每日皮下注射,连续21 d,制作继发性淀粉样变性小鼠动物模型,对照组连续注射21 d 0.5 ml生理盐水;2组小鼠各4只,经尾静脉注射200μl(7.4 MBq)131I-SAP,分别于1,3,6,24,48和72 h进行显像.实验组、对照组小鼠各30只,经尾静脉注射100μl(555 kBq)131 I-SAP后,分别于1,3,6,24,48和72 h处死小鼠(每个时相实验组与对照组各5只),取心、肺、肝、脾、肾、肌肉、主动脉、血液等组织,测定放射性计数.两组间比较采用单因素独立样本t检验.结果 131 I-SAP的标记率为70.6%,经分离纯化后,放化纯为(95.5±3.4)%,且稳定性好;1311I-SAP在淀粉样变性小鼠肝、脾与肾中的放射性摄取明显高于对照组,实验组24h单位质量放射性计数肝/血、脾/血、肾/血比值分别为2.201±0.301,2.139±0.223,4.797±0.615,对照组分别为0.657±0.126,1.014±0.063,0.607±0.028,t值分别为10.747,11.626和15.135,P均<0.01.48 h二者差异仍具有统计学意义(t值分别为15.128,4.558,16.960,P均<0.01);72 h 2组除脾/血比值外,其余2个比值差异有统计学意义,对应t值为3.022(P >0.05),7.8011,6.442(P均<0.01).显像结果示,注药后24h实验组小鼠腹部放射性摄取增加,而对照组无明显摄取.结论 SAP易于进行131I标记,且标记物稳定性好;1311 I-SAP可以特异性结合于淀粉样变性脏器,生物分布与显像结果一致,提示131I-SAP可以作为特异性的显像剂无创诊断淀粉样变性. Objective To validate 131 I-serum amyloid P-component (SAP) as a novel amyloid probe in mice bearing amyloidosis and evaluate its diagnostic value.Methods Standard SAP was labeled with 131I using Iodogen method.Amyloidotic mice model was established by subcutaneous injection of 0.5 ml 10% Casein daily for 21 d,and the control group was injected with 0.5 ml saline.Both groups were injected with 7.4 MBq131 I-SAP through the tail veins and imaging was performed at 1,3,6,24,48 and 72 h post injection.In biodistribution study,30 amyloidotic mice model and 30 controls were injected with 555 kBq 131 I-SAP and were killed evenly at 1,3,6,24,48 and 72 h post injection.T test was used to analysis the data.Results The labeling efficiency of 131 I-SAP was 70.6%,and the radiochemical purity was ( 95.5 ±3.4) %.There was significant tracer uptake by liver,spleen and kidney at 24 h in the test group and mild uptake by these organs in the control group.The uptake ratios of liver,spleen and kidney over blood in the test group were 2.201 ±0.301,2.139 ±0.223,4.797 ±0.615,vs 0.657 ±0.126,1.014 ±0.063,0.607 ±0.028 in the control group,respectively (t =10.747,11.626 and 15.135,all P <0.01).The uptake differences between the two groups were still statistically significant at 48 h ( t =15.128,4.558,16.960,all P < 0.01 ).The uptake ratios of spleen over blood between the two groups were not significantly different at 72 h ( t =3.022,P > 0.05 ),but the other two uptake ratios were both sigificantly different ( t =7.801,6.442,both P < 0.01 ).Conclusions SAP can be reliably labeled with 131I.The labeled product 131 I-SAP can accumulate in amyloid laden tissues,thus rendering it a potential agent in the detection of amyloidosis.
语种: 中文
原文出处: 查看原文
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/43181
Appears in Collections:北京大学第一临床医学院_核医学科_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 100034,北京大学第一医院核医学科
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[魏海亮]'s Articles
[张春丽]'s Articles
[王荣福]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[魏海亮]‘s Articles
[张春丽]‘s Articles
[王荣福]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace