北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第一临床医学院  > 病理科  > 期刊论文
学科主题: 病理学
题名:
C型凝集素样自然杀伤(NK)细胞受体在结外鼻型NK/T细胞淋巴瘤中表达及意义
其他题名: Study on expression of natural killer (NK) cell C-type lectin-like receptors in nasal NK/T-cell lymphomas
作者: 农琳; 张爽; 李阳; 张莹; 王颖; 李挺
关键词: 淋巴瘤,T细胞,外周 ; 外源凝集素类,C型 ; 抗原,CD94 ; 杀伤细胞,天然 ; Lymphoma,T-cell,peripheral ; Lectins,C-type ; Antigens,CD94 ; Killer cells,natural
刊名: 中华病理学杂志
发表日期: 2010
DOI: 10.3760/cma.j.issn.0529-5807.2010.05.007
卷: 39, 期:5, 页:319-324
收录类别: 中国科技核心期刊 ; 中文核心期刊 ; CSCD
文章类型: Journal Article
摘要: 目的 探讨C型凝集素样自然杀伤(NK)细胞受体CD94和NKG2在结外鼻型NK/T细胞淋巴瘤中的表达及意义.方法 运用逆转录聚合酶链反应(RT-PCR)检测C型凝集素样NK细胞受体CD94和NKG2在经组织形态、免疫组织化学、EB病毒原位杂交及T细胞受体PCR克隆性重排分析确诊的21例结外鼻型NK/T细胞淋巴瘤以及对照组同部位B细胞淋巴瘤8例、淋巴结外周T细胞淋巴瘤(PTCL)10例、脾脏5例、胸腺5例和慢性炎性鼻黏膜5例组织中的表达情况并进行随访.结果 21例结外鼻型NK/T细胞淋巴瘤具有典型的形态学改变,表达CD3ε、CD56和细胞毒蛋白,TCR重排阴性,20例EB病毒阳性;RT-PCR扩增结果显示,在21例结外鼻型NK/T细胞淋巴瘤中,18例(85.7%)CD94呈阳性表达;NKG2总阳性率为95.2%(20/21),各亚基表达阳性率依次为NKG2A/2B(85.7%)、NKG2D(61.9%)、NKG2F(14.3%)、NKG2C,/2E(4.8%).对照组中PTCL和B细胞淋巴瘤均不表达CD94和NKG2,仅2例脾脏和2例慢性炎性鼻黏膜组织表达CD94,1例脾脏组织表达NKG2A/2B,1例胸腺组织表达NKG2D.CD94和NKG2在结外鼻型NK/T细胞淋巴瘤中的表达与T细胞淋巴瘤和B细胞淋巴瘤比较,差异均有统计学意义(P均<0.01).CD94和NKG2同时表达于17例结外鼻型NK/T细胞淋巴瘤,共表达率为81.0%(17/21).结论 CD94和NKG2在结外鼻型NK/T细胞淋巴瘤中呈特异性和顺序性表达,提示多数病例肿瘤细胞处于活化和功能NK细胞阶段.对这些分子的检测有可能成为NK细胞源性淋巴瘤诊断的重要手段. Objective To investigate the expression and possible role of C-type lectin-like natural killer cell receptors, including CD94 and NKG2s, in extranodal NK/T-cell lymphoma, nasal type (EN-NK/ T-NT). Methods Reverse transcriptase polymerase chain reaction (RT-PCR) was used to detect the expression of CD94 and NKG2s in tissue sections of 21 cases of EN-NK/T-NT (confirmed by histology, immunohistochemistry, in-situ hybridization for Epstein-Barr virus ( EBV ) and PCR for T-cell receptor genes) , eight midline B cell lymphomas ( BCL) , 10 peripheral T cell lymphoma of lymph nodes ( PTCL) , five spleens, five thymuses and five chronic nasopharyngitis. Results All 21 cases of EN-NK/T-NT showed typical histological features,with expression of CD38,CD56, cytotoxic granules and positivity of EBV in 20 cases. The RT-PCR results showed a high level expression of CD94 (85. 7% ) and NKG2 members (95. 2% totally, with NKG2A/2B in 85.7%, NKG2D in 61.9%, NKG2F in 14.3%, NKG2C/2E in 4.8%, respectively and sequentially) in EN-NK/T-NT. But in the controls, none of the receptors were detected in TCL(0% ) and BCL(0%), while only a few cases of lymphoid tissues expressed one or two of these receptors (two spleens and two chronic nasopharyngitis mucosa for CD94, one spleen for NKG2A/2B and one thymus for NKG2D). The differences of CD94 and NKG2 expression between EN-NK/T-NT and BCL or TCL were statistically significant (P <0. 01). Co-expression of CD94 and NKG2 was found in 17 out of 21 EN-NK/T-NT cases (81. 0% ). Conclusions The specific and sequential expression nature of CD94 and NKG2 in EN-NK/T-NT, mimicks the developmental expression model in their normal counterparts, andsuggests that the tumor cells of most cases are being activated and keeping in a stage as the functional NK cells. Detection of these molecules may provide a useful tool to confirm the diagnosis of NK cell lymphoma.
语种: 中文
原文出处: 查看原文
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/43327
Appears in Collections:北京大学第一临床医学院_病理科_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 北京大学第一医院病理科,100034

Recommended Citation:
农琳,张爽,李阳,等. C型凝集素样自然杀伤(NK)细胞受体在结外鼻型NK/T细胞淋巴瘤中表达及意义[J]. 中华病理学杂志,2010,39(5):319-324.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[农琳]'s Articles
[张爽]'s Articles
[李阳]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[农琳]‘s Articles
[张爽]‘s Articles
[李阳]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace