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学科主题: 病理学
题名:
结外NK/T细胞淋巴瘤(鼻型)microRNA的表达研究
其他题名: Expression of microRNA in extranodal NK/T cell lymphoma, nasal type
作者: 遆红娟1; 农琳1; 王微1; 张爽1; 李挺1
关键词: 淋巴瘤,T细胞,外周 ; 微RNAs ; 聚合酶链反应 ; Extranodal NK/T cell lymphoma,nasal type ; microRNAs ; Polymerase chain reaction
刊名: 中华病理学杂志
发表日期: 2011
DOI: 10.3760/cma.j.issn.0529-5807.2011.09.008
卷: 40, 期:9, 页:610-615
收录类别: 中国科技核心期刊 ; 中文核心期刊 ; CSCD
文章类型: Journal Article
摘要: 目的 通过分析结外NK/T细胞淋巴瘤(鼻型)肿瘤组织中microRNA( miRNA)的表达情况,探索其分子改变特征。方法 对1例结外NK/T细胞淋巴瘤(鼻型)和1例炎性鼻黏膜组织应用TaqMan低密度芯片分析665种miRNA的表达差异,发现95种miRNA存在变化。结合文献复习和数据库检索,从其中筛选差异明显、重要的8种miRNA。应用即时定量聚合酶链反应方法进一步对15例结外NK/T细胞淋巴瘤(鼻型)与3例炎性鼻黏膜组织验证这8种miRNA的表达情况。结果 8种miRNA验证结果与表达谱结果对比表达趋势一致,经统计学处理,其中3种重要miRNA[miR-223和miR-886-3p在结外NK/T细胞淋巴瘤(鼻型)组织中表达上调,miR-34c-5p表达下调]表达差异有统计学意义(P值分别为0.002、0.010和0.017)。结论 造血系分化发育、细胞增殖凋亡有关的miRNA:miR-223、886-3p和34c-5p在结外NK/T细胞淋巴瘤(鼻型)表达有明显异常,其是否参与肿瘤分子遗传学的改变及其意义值得深入探讨。 Objective To study the expression of microRNAs (miRNAs) in extranodal NK/T cell lymphoma, nasal type (EN-NK/T-NT). Methods TaqMan low density arrays were used to assess the expression level of 665 miRNAs in one case of EN-NK/T-NT and one normal nasopharyngeal tissue. Ninetyfive miRNAs were found to be aberrantly expressed (86 being up-regulated and 9 being down-regulated) in EN-NK/T-NT, compared with normal nasopharyngeal tissue. The aberrant expression was found to be most significant in 8 miRNAs. According to the literature and miRNA database, the expression patterns of these 8 miRNAs were further analyzed in 15 cases of EN-NK/T-NT and 3 normal nasopharyngeal specimens, using the single tube TaqMan microRNA assays. Results Three of the 8 miRNAs showed statistically significant difference in the expression in EN-NK/T-NT and normal nasopharyngeal specimens. These 3 miRNAs ( miR-223, 886-3p and 34c-5p) were considered to play crucial roles in hemopoiesis, cellular proliferation and apoptosis. MiR-223 and miR-886-3p were significantly over-expressed in EN-NK/T-NT ( P =0. 002, P =0. 010) while miR-34c-5p was significantly under-expressed (P =0. 017). Conclusions Certain types of miRNAs, especially those related to hemopoiesis, cellular proliferation and apoptosis, are aberrantly expressed in EN-NK/T-NT. The potential role of miRNAs in the molecular genetics of EN-NK/T-NT requires further investigation.
语种: 中文
原文出处: 查看原文
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内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/43335
Appears in Collections:北京大学第一临床医学院_病理科_期刊论文

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作者单位: 1.100034,北京大学第一医院病理科
2.中日友好医院血液科

Recommended Citation:
遆红娟,农琳,王微,等. 结外NK/T细胞淋巴瘤(鼻型)microRNA的表达研究[J]. 中华病理学杂志,2011,40(9):610-615.
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