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学科主题: 病理学
题名:
子宫内膜癌微小RNA的表达特点及其临床意义
其他题名: MicroRNA expression signature profile and its clinical significance in endometrioid carcinoma
作者: 王颖1; 阿迪拉1; 斯依提1; 张晓明1; 董颖1; 李文婷1; 周梅1; 李挺1
关键词: 子宫内膜肿瘤 ; 微RNAs ; PTEN磷酸水解酶 ; Endometrial neoplasms ; MicroRNAs ; PTEN phosphohydrolase
刊名: 中华病理学杂志
发表日期: 2014
DOI: 10.3760/cma.j.issn.0529-5807.2014.02.004
卷: 43, 期:2, 页:88-94
收录类别: 中国科技核心期刊 ; 中文核心期刊 ; CSCD
文章类型: Journal Article
摘要: 目的 探讨典型子宫内膜样癌组织微小RNA(miRNA)的表达特点及与子宫内膜癌相关分子改变的内在关联性及其临床意义.方法 以TaqMan低密度芯片分析2例子宫内膜样癌组织miRNA的表达特点.筛选表达显著差异的miRNA,采用即时荧光定量聚合酶链反应技术检测73例子宫内膜癌组织中这些miRNA的表达.免疫组织化学法检测miRNA的预测靶基因PTEN表达.结果 (1) miRNA表达谱分析显示相对于增殖期内膜,典型Ⅰ型癌组织共有47个miRNA存在差异表达,其中26个表达降低,21个表达升高.(2)进一步筛选8种miRNA验证其表达:8种miRNA在58例Ⅰ型癌中的表达与表达谱结果一致,其中表达升高的3种miRNA(miR-141、miR-200a、miR-205)及表达降低的两种miRNA(miR-143和miR-145),差异均有统计学意义(P<0.05),但此5种miRNA在Ⅱ型内膜癌中变化不显著.(3)Ⅰ型内膜癌中miR-141、miR-200a高表达组的PTEN失表达率更高,且miR-200a与PTEN表达呈负相关(P<0.05).结论 Ⅰ型子宫内膜癌有相对特异的miRNA表达谱;miR-200a、miR-205、miR-141、miR-143、miR-145在Ⅰ型癌中变化显著,但在Ⅱ型癌中变化不显著,提示Ⅰ/Ⅱ型癌发病中发挥作用的miRNA不尽相同.Ⅰ型癌中miR-141和miR-200a可能通过对抑癌基因PTEN的靶向调控,参与子宫内膜癌的形成及演进. Objective To determine the microRNA (miRNA) expression signature and its clinical significance in endometrioid carcinoma (EC).Methods The miRNA profiles were analyzed by miRNA microarray in 73 cases of EC.The expression level of the eight selected miRNAs were measured by real-time fluorescent quatitative PCR(qRT-PCR).Immunohistochemistry (IHC) was performed to assess the status of PTEN,a potential target of the selected miRNAs.Results (1) Using TaqMan low-density arrays,47 miRNAs that differed between EC and normal controls were identified,including 26 down-regulated and 21 up-regulated miRNAs.(2) To confirm the miRNA expression pattern in type Ⅰ EC,the expression levels of the eight selected miRNAs were evaluated in a new set of 58 cases of type Ⅰ EC by individual miRNA qRT-PCR assays.Three miRNAs (miR-141,miR-200a,miR-205) were up-regulated and two miRNAs (miR-143,miR-145) were down-regulated.These were significantly differentially expressed in type Ⅰ EC and normal controls (P < 0.05),whereas such difference was not present in type Ⅱ tumors compared to normal controls.(3) In type Ⅰ EC,loss of PTEN was more frequent in the miR-141 or miR-200a up-regulated subgroups,and the correlation between the PTEN and miR-200a status in type Ⅰ tumors was statistically significant (P <0.05).Conclusions EC may have a unique miRNA expression profile.The expression levels of the five miRNAs (miR-141,miR-200a,miR-205,miR-143,miR-145) are significantly deregulated in type Ⅰ EC compared to normal control but not in type Ⅱ tumors.The findings suggest that the miRNAs related to type Ⅰ and type Ⅱ EC might be different.PTEN might be a potential target of miR-141 and miR-200a in endometrial carcinogenesis.
语种: 中文
原文出处: 查看原文
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内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/43337
Appears in Collections:北京大学第一临床医学院_病理科_期刊论文

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作者单位: 1.100034,北京大学第一医院病理科
2.新疆医科大学附属肿瘤医院病理科,乌鲁木齐,830011

Recommended Citation:
王颖,阿迪拉,斯依提,等. 子宫内膜癌微小RNA的表达特点及其临床意义[J]. 中华病理学杂志,2014,43(2):88-94.
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