北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第一临床医学院  > 检验科  > 期刊论文
学科主题: 临床检验诊断学
题名:
ADVIA 120/2120及多种类血细胞分析仪复检规则的建立和评价
其他题名: Establishment and evaluation of review criteria for ADVIA 120/2120 and different series of hematology analyzers
作者: 邢莹1; 王建中1; 普程伟1; 尚柯1; 闫振林1; 白玉柱1; 杨文双1; 李涛1; 张时民1
关键词: 设备和供应 ; 血细胞计数 ; 自动化 ; 参考标准 ; 评价研究 ; Equipment and supplies ; Blood cell count ; Automation ; Reference standards ; Evaluation studies
刊名: 中华医学杂志
发表日期: 2010
DOI: 10.3760/cma.j.issn.0376-2491.2010.22.006
卷: 90, 期:22, 页:1526-1530
收录类别: 中国科技核心期刊 ; 中文核心期刊 ; CSCD
文章类型: Journal Article
摘要: 目的 建立ADVIA 120/2120血细胞分析仪复检规则和不同系列血细胞分析仪的通用复检规则.方法 应用ADVIA120/2120血细胞分析仪检测2400份标本(含恶性血液病标本6份),其中1200份标本同时应用Sysmex XE-2100和Beckman-Coulter LH750血细胞分析仪检测,并均进行血涂片显微镜检查(镜检),对检测结果进行统计分析,参考国际血细胞分析仪复检规则并进行适当调整建立新的复检规则.随机选择300份标本验证新建立的复检规则.结果 根据国际血细胞分析仪复检规则和我国血液学专家制定的涂片镜检阳性标准进行统计分析,ADVIA 120/2120血细胞分析仪检测的2400份标本真阳性率22.1%(530份),假阳性率28.1%(675份),真阴性率44.3%(1063份),假阴性率5.5%(132份),复检率50.2%(1205份),假阴性率超出最大可接受限(5.0%).修改涂片镜检阳性标准(提高中性杆状核粒细胞、嗜酸粒细胞、嗜碱粒细胞和单核细胞百分比)并对国际血细胞分析仪复检规则进行适当调整后建立新的复检规则,重新进行统计分析,真阳性率为15.5%(371份),假阳性率18.7%(449份),真阴性率61.6%(1479份),假阴性率4.2%(101份),复检率34.2%(821份),恶性血液病标本无漏检.在ADVIA 120/2120血细胞分析仪复检规则的基础上添加Sysmex XE-2100和Beckman-Coulter LH750血细胞分析仪特异参数后提出了通用复检规则,统计分析3种血细胞分析仪同时测定的1200份标本,假阴性率分别为4.3%、4.6%和4.6%,假阳性率分别为14.7%、17.5%和12.7%,均未漏检恶性血液病标本.验证试验中3种仪器的假阴性率分别为3.8%、4.3%和4.0%.结论 建立了适用于大型综合性医院ADVIA 120/2120血细胞分析仪临床应用的复检规则和适用于3种不同系列血细胞分析仪的通用复检规则. objective To establish the suitable review criteria for ADVIA 120/2120 and those for different series of hematology analyzers.Methods A total of 2400 samples,including 6 blood neoplasms,were detected with ADVIA 120/2120 hematology analyzer,in which 1200 samples were detected by Sysmex XE-2100 and Beckman-Coulter LH750 hematology analyzers.In the meantime,blood smears were reviewed,and the results were analyzed statistically.The new review criteria were established by consulting and modifying the one as recommended by an international consensus group.Finally 300 samples were selected to validate the new review criteria.Results The results of 2400 samples detected by ADVIA 120/2120 hematology analyzer were analyzed statistically according to the international consensus review rules and blood smear positive criteria formulated by Chinese experts.The true positive rate was 22.1%(n=530),false positive rate 28.1%(n=675),true negative rate 44.3%(n=1063),false negative rate 5.5%(n=132),and the smear review rate 50.2%(n=1205).The false negative rate was over the acceptable limit of 5%.The new review criteria were established by amending the blcod smear positive criteria,i.e.increasing the percentage of band neutrophils,eosinophils,basophils and monocytes and adjusting the international consensus review rules.Then the results were re-analyzed.The true positive rate.false positive rate,true negative rate and false negative rate were 15.5%(n=371),18.7%(n=449),61.6%(n=1479) and 4.2%(n=101) respectively.The smear review rate was 34.2%(n=821) and no specimen of blood neoplasms was missed.On that basis,the current review criteria for ADVIA 120/2120,XE-2100 and LH750 hematology analyzer were proposed by adding some specific parameters.The results of 1200 samples with three instruments were analyzed according to the current criteria.And the false negative rates were 4.3%,4.6% and 4.6%,and false positive rate 14.7%,17.5%and 12.7% respectively.And no specimen of blood neoplasm Was missed.The false negative rates of three instruments were 3.8%,4.3% and 4.0% in validation teses.Conclusion The review criteria for three difierent series of hematology analyzers have been formulated for large general hospitals.
语种: 中文
原文出处: 查看原文
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/43736
Appears in Collections:北京大学第一临床医学院_检验科_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.北京大学第一医院检验科,100034
2.中国医学科学院北京协和医院检验科

Recommended Citation:
邢莹,王建中,普程伟,等. ADVIA 120/2120及多种类血细胞分析仪复检规则的建立和评价[J]. 中华医学杂志,2010,90(22):1526-1530.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[邢莹]'s Articles
[王建中]'s Articles
[普程伟]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[邢莹]‘s Articles
[王建中]‘s Articles
[普程伟]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace