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学科主题: 临床医学
题名:
爪蟾视觉发育过程中抑制性GABAa受体功能的变化
其他题名: Variation of mIPSCs mediated by GABAa receptors during visual development in Xenopus
作者: 王红; 温小红; 蔡浩然
关键词: 视顶盖 ; GABA能受体 ; 爪蟾 ; 发育 ; 电压钳 ; 成熟 ; optic tectum ; GABAergic receptor ; Xenopus ; development ; voltage-clamp ; maturation
刊名: 中国神经科学杂志
发表日期: 2003
卷: 19, 期:4, 页:216-222
文章类型: Journal Article
摘要: 目的:阐明从幼年(变态脱尾后2~12周)到成年(变态脱尾后5~10月)爪蟾的视顶盖神经元突触受体变化.方法:盲法脑片全细胞电压钳技术.结果:成年视顶盖神经元的微抑制性突触后电流(mIPSCs)的发放频率(Hz)和平均振幅(pA)分别为2.65±0.69和9.82±1.30,而幼年的分别为0.68±0.23和15.36±2.40,其中成年动物的mIPSCs的频率是幼年频率的3.90倍;乙酰胆碱受体(nAChR)激动剂carbachol均可使幼年及成年的mIPSCs频率增加,增强效率有所不同,幼年增加至对照组的192.0%,而成年组仅为146.2%.结论:随着视顶盖神经元的成熟,其突触GABAa受体功能也相应增强. Objective: To characterize the receptors located in the presynaptic and postsynaptic membranes of tectalneurons in juvenile (2 to 12 weeks post-metamorphosis) and adult (5 ~ 10 months post-metamorphosis) Xenopus. Meth-ods: Using the blind technique, whole-cell patch-clamp recordings were made from layer Ⅵ of a slice preparation of theXenopus optic tectum in vitro. Results: The mIPSCs recorded from the tectal neurons in the adult Xenopus ( n = 57 ) andin the juvenile ( n = 32) were completely abolished by bath application of the GABAa antagonist bicuculline. The frequen-cy of mIPSCs recorded from the adult Xenopus (2.65 Hz ± 0.69 Hz) was 3.90 fold higher than that of the juvenile animals( 0.68 Hz ± 0.23 Hz), but the averaged amplitude of the former (9.82 pA ± 1.30 pA) was smaller than that of the latter(15.36 pA +2.40 pA). The mIPSCs were modulated presynaptically by nACh receptors. nAChR agonists such as carba-chol could induce an enhancement in the mIPSC frequency. The enhancement of mIPSC frequency induced by carbachol injuvenile Xenopus ( 192.0 % of control) was stronger than that in older animals ( 146.2% of control). Conclusion: With the maturation of tectal neuron, the functional GABAa receptors increased progressively.
语种: 中文
原文出处: 查看原文
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/44058
Appears in Collections:北京大学第一临床医学院_中心实验室_期刊论文

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作者单位: 北京大学第一医院中心实验室,北京,100034

Recommended Citation:
王红,温小红,蔡浩然. 爪蟾视觉发育过程中抑制性GABAa受体功能的变化[J]. 中国神经科学杂志,2003,19(4):216-222.
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