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学科主题: 临床医学
题名:
非洲爪蟾顶盖神经元的抑制性微突触后电流频率和振幅的电压依赖性
其他题名: Voltage-dependence of miniature inhibitory postsynaptic current frequency and amplitude in tectal neurons of Xenopus
作者: 朱丹1; 艾育德1; 蔡浩然1
关键词: 电压依赖性 ; 抑制性微突触后电流 ; 视顶盖神经元 ; 脑片 ; 去极化 ; 膜电位 ; 非洲爪蟾 ; voltage-dependence ; miniature inhibitory postsynaptic current ; tectal neuron ; slice ; depolarization ; membrane potential ; Xenopus
刊名: 生理学报
发表日期: 2006
DOI: 10.3321/j.issn:0371-0874.2006.01.004
卷: 58, 期:1, 页:21-28
收录类别: 中国科技核心期刊 ; 中文核心期刊 ; CSCD
文章类型: Journal Article
摘要: 采用全细胞记录膜片钳技术,研究非洲爪蟾脑片视顶盖神经元微突触后电流(miniatureinhibitory postsynaptic current,mIPSC)频率和振幅对电压依赖关系.观察到以下结果:(1)当通过改变记录电极内的DC电流,将神经元的膜电位从静息电位逐步(每步10 mV的增量)去极化或超极化时,mIPSC的频率和/或振幅分别升高或降低.随着膜电位的去极化,mIPSC的频率逐渐升高;当钳位电压升至+10 mV时,mIPSC的频率达到最高值.(2)当神经元去极化时,振幅仅轻微升高.膜电位去极化达到-30 mV或-40 mV时,mIPSC的振幅最大;进一步去极化,振幅反而下降.另外,在膜电位去极化至-20 mV和+10 mV之间时,可记录到大的mIPSC.(3)在无Ca2+浸浴液中,mIPSC的频率和振幅也随膜电位的去极化而逐步增高,但频率的增高幅度远不如在生理盐水浸浴中增高幅度明显.(4)当浸浴液中[K+]o增高时,mIPSC的频率明显降低,而振幅轻微降低.当细胞外[K+]o浓度升高超过20 mmol/L时,神经元产生明显的缓慢内向或外向膜电流.mIPSC频率和振幅与膜电位存在依赖性的可能机制在文中作了简短的讨论. Experiments were performed to study the voltage-dependence of miniature inhibitory postsynaptic current (mIPSC)frequency and amplitude using patch-clamp technique with whole cell recording in optic tectal slices of Xenopus. The following results have been observed. (1) When the membrane potentials of a neuron were depolarized or hyperpolarized stepwise from a resting potential via recording pipette to inject a DC current, the frequency and/or amplitude of mIPSCs increased or decreased respectively.The frequency of mIPSCs increased gradually with depolarizing membrane potential and it attained to the maximum as the membrane potential was held at +10 mV. (2) The amplitude increased slightly as the neuron was depolarized. When the depolarization of membrane potential reached -30 or -40 mV, the amplitudes of mIPSCs were maximal. Further depolarization resulted in a decrease of amplitude. Meanwhile, the large mIPSCs appeared when the membrane potential depolarized to a range between -20 mV and +10 mV.(3) With Ca2+-free bath solution, the frequency and amplitude of mIPSCs also increased stepwise progressively on depolarization of membrane potential, but the increase was less marked as corresponding value in normal saline perfusion. (4) When the [K+]o in bath solution increased, the frequency of mIPSCs decreased markedly and the amplitude of mIPSCs decreased slightly. If the external K+ concentration increased further to higher than 20 mmol/L, the neuron produced a marked slow inward or outward membrane current.The possible mechanism underlying the voltage-dependence of mIPSC frequency and amplitude is discussed briefly.
语种: 中文
原文出处: 查看原文
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内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/44070
Appears in Collections:北京大学第一临床医学院_中心实验室_期刊论文

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作者单位: 1.北京大学第一医院中心实验室,北京,100034
2.内蒙古医学院附属医院眼科,呼和浩特,010050
3.内蒙古医学院附属医院眼科,呼和浩特,010050
4.北京大学第一医院中心实验室,北京,100034

Recommended Citation:
朱丹,艾育德,蔡浩然. 非洲爪蟾顶盖神经元的抑制性微突触后电流频率和振幅的电压依赖性[J]. 生理学报,2006,58(1):21-28.
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