IR@PKUHSC  > 北京大学第一临床医学院  > 临床药理研究所
学科主题药理学
高效液相色谱测定比格犬血浆中的头孢唑啉与头孢曲松的浓度
其他题名Determination of cefazolin and ceftriaxone in Beagle dog plasma by high performance liquid chromatography
李天云
关键词高效液相色谱法 头孢唑啉 头孢曲松 比格犬 血浆 Hplc Cefazolin Ceftriaxone Beagle Dog Plasma
刊名中国临床药理学杂志
2015
DOI10.13699/j.cnki.1001-6821.2015.14.029
14页:1452-1454
收录类别中国科技核心期刊 ; 中文核心期刊 ; CSCD
文章类型Journal Article
摘要目的:建立HPLC在多项测定参数相同的基础上,分别测定比格犬血浆中头孢唑啉、头孢曲松浓度的方法。方法色谱柱:头孢唑啉为ES industries Epic C18(4.6 mm ×150 mm,5μm),头孢曲松为Phenomenex Gemini C18(4.6 mm ×150 mm,5μm ),流动相:头孢唑啉为甲醇-0.05 mol? L-1乙酸铵缓冲液( pH=4.3)=30∶70;头孢曲松为乙腈-0.05 mol? L-1 KH2 PO4缓冲液( pH=6.0)=6∶94,流速均为1.0 mL? min-1,进样量均为40μL。结果头孢唑啉标准曲线回归方程为 y =-1.25×104 x +8479.37( r =0.9974),头孢曲松为y=1.25×104 x +2.72×104( r =0.9976)。头孢唑啉线性范围为2~300μg? mL-1,头孢曲松为2~200μg? mL-1;日内、日间精密度相对标准偏差均≤10%,提取回收率均≥85.78%。结论本法灵敏、准确、快速,可用于比格犬血浆头孢唑啉、头孢曲松浓度的测定和药物其他方面研究。 Objective To develop a HPLC method for the determination of cefazolin and ceftriaxone in vivo.Methods Ceftriaxone use Phenome-nex Gemini C18 (4.6 mm ×150 mm, 5 μm) and cefazolin use ES indus-tries EpicC18 (4.6 mm ×150 mm, 5 μm) columns,all were used at 30℃.Cefazolin with a mobile phase of( pH=4.3 ) 0.05 mol? L-1 ammo-nium acetate -methanol solution ( 70∶30 ) , ceftriaxone with a mobile phase of ( pH =6.0 ) 0.05 mol? L-1 phosphater buffer -acetonitrile (94∶6).All at a flow-rate of 1.0 mL? min -1 .Injection volume of 40μL all were used .Quantitaion was performed using HPLC.Results The straight line corresponding to equation were obtained:y=-1.25 ×104 x+8479.37( r=0.997 4 ) in the range of 2 -300 μg? mL-1 for cefazolin;and y=1.25 ×104 x+2.72 ×104 ( r=0.997 6) in the range of 2-200μg? mL-1 for ceftriaxone. The extraction recovery for cefazolin and ceftriaxone were ≥ 85.78% .The intra and inter day precision were ≤10%.Conclusion The analytical method appeared to be accu-rate, sensitive and convenient for the determineation of cefazolin and ceftriaxone in Beagle dog.It is suitable for animal pharmacokinetic study of cefazolin and ceftriaxone or in other fields.
语种中文
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文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/44387
Collection北京大学第一临床医学院_临床药理研究所
作者单位北京大学 第一医院 临床药理研究所,北京,100034
Recommended Citation
GB/T 7714
李天云. 高效液相色谱测定比格犬血浆中的头孢唑啉与头孢曲松的浓度[J]. 中国临床药理学杂志,2015(14):1452-1454.
APA 李天云.(2015).高效液相色谱测定比格犬血浆中的头孢唑啉与头孢曲松的浓度.中国临床药理学杂志(14),1452-1454.
MLA 李天云."高效液相色谱测定比格犬血浆中的头孢唑啉与头孢曲松的浓度".中国临床药理学杂志 .14(2015):1452-1454.
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