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学科主题: 药理学
题名:
高效液相色谱测定比格犬血浆中的头孢唑啉与头孢曲松的浓度
其他题名: Determination of cefazolin and ceftriaxone in Beagle dog plasma by high performance liquid chromatography
作者: 李天云
关键词: 高效液相色谱法 ; 头孢唑啉 ; 头孢曲松 ; 比格犬 ; 血浆 ; HPLC ; cefazolin ; ceftriaxone ; Beagle dog ; plasma
刊名: 中国临床药理学杂志
发表日期: 2015
DOI: 10.13699/j.cnki.1001-6821.2015.14.029
期: 14, 页:1452-1454
收录类别: 中国科技核心期刊 ; 中文核心期刊 ; CSCD
文章类型: Journal Article
摘要: 目的:建立HPLC在多项测定参数相同的基础上,分别测定比格犬血浆中头孢唑啉、头孢曲松浓度的方法。方法色谱柱:头孢唑啉为ES industries Epic C18(4.6 mm ×150 mm,5μm),头孢曲松为Phenomenex Gemini C18(4.6 mm ×150 mm,5μm ),流动相:头孢唑啉为甲醇-0.05 mol? L-1乙酸铵缓冲液( pH=4.3)=30∶70;头孢曲松为乙腈-0.05 mol? L-1 KH2 PO4缓冲液( pH=6.0)=6∶94,流速均为1.0 mL? min-1,进样量均为40μL。结果头孢唑啉标准曲线回归方程为 y =-1.25×104 x +8479.37( r =0.9974),头孢曲松为y=1.25×104 x +2.72×104( r =0.9976)。头孢唑啉线性范围为2~300μg? mL-1,头孢曲松为2~200μg? mL-1;日内、日间精密度相对标准偏差均≤10%,提取回收率均≥85.78%。结论本法灵敏、准确、快速,可用于比格犬血浆头孢唑啉、头孢曲松浓度的测定和药物其他方面研究。 Objective To develop a HPLC method for the determination of cefazolin and ceftriaxone in vivo.Methods Ceftriaxone use Phenome-nex Gemini C18 (4.6 mm ×150 mm, 5 μm) and cefazolin use ES indus-tries EpicC18 (4.6 mm ×150 mm, 5 μm) columns,all were used at 30℃.Cefazolin with a mobile phase of( pH=4.3 ) 0.05 mol? L-1 ammo-nium acetate -methanol solution ( 70∶30 ) , ceftriaxone with a mobile phase of ( pH =6.0 ) 0.05 mol? L-1 phosphater buffer -acetonitrile (94∶6).All at a flow-rate of 1.0 mL? min -1 .Injection volume of 40μL all were used .Quantitaion was performed using HPLC.Results The straight line corresponding to equation were obtained:y=-1.25 ×104 x+8479.37( r=0.997 4 ) in the range of 2 -300 μg? mL-1 for cefazolin;and y=1.25 ×104 x+2.72 ×104 ( r=0.997 6) in the range of 2-200μg? mL-1 for ceftriaxone. The extraction recovery for cefazolin and ceftriaxone were ≥ 85.78% .The intra and inter day precision were ≤10%.Conclusion The analytical method appeared to be accu-rate, sensitive and convenient for the determineation of cefazolin and ceftriaxone in Beagle dog.It is suitable for animal pharmacokinetic study of cefazolin and ceftriaxone or in other fields.
语种: 中文
原文出处: 查看原文
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内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/44387
Appears in Collections:北京大学第一临床医学院_临床药理研究所_期刊论文

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作者单位: 北京大学 第一医院 临床药理研究所,北京,100034

Recommended Citation:
李天云. 高效液相色谱测定比格犬血浆中的头孢唑啉与头孢曲松的浓度[J]. 中国临床药理学杂志,2015(14):1452-1454.
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