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学科主题: 药理学
题名:
亚胺培南诱导耐药鲍曼不动杆菌adeB表达变化研究
其他题名: Expression of adeB mRNA in imipenem induced carbapenem resistant Acinetobacter baumannii clinical isolates
作者: 马序竹; 李湘燕; 薛峰; 张佳; 吕媛
关键词: 鲍曼不动杆菌 ; 碳青霉烯耐药 ; AdeABC ; 外排泵 ; A.baumannii ; carbapenem resistance ; AdeABC ; efflux pump
刊名: 中国临床药理学杂志
发表日期: 2014
DOI: 10.3969/j.issn.1001-6821.2014.08.013
期: 8, 页:696-700
收录类别: 中国科技核心期刊 ; 中文核心期刊 ; CSCD
文章类型: Journal Article
摘要: 目的:明确AdeABC外排系统在亚胺培南获得性耐药鲍曼不动杆菌中的作用。方法用试管二倍稀释法对6株亚胺培南敏感鲍曼不动杆菌进行体外诱导;用琼脂稀释法测定10种抗菌药物及加入外排泵抑制剂氰氯苯腙( CCCP )12.5 mg· L-1后菌株的耐药表型。脉冲场凝胶电泳对菌株进行分型。用PCR及测序方法检测相关耐药基因。 Real -time PCR 方法检测菌株 blaAdeB及blaOXA-51-like mRNA表达量变化。结果6株鲍曼不动杆菌为亚胺培南及美罗培南敏感的多药耐药菌株。经亚胺培南体外诱导后,4株菌对亚胺培南的最低抑菌浓度( MIC)值有4倍以上增加,成为亚胺培南耐药菌株。在加入CCCP后,对亚胺培南及美罗培南的MIC值下降到1/2~1/8。6株菌均携带blaOXA-66基因。blaoxa-23-like、blaoxa-24-like、blaoxa-58-like、blaimp及blavim基因检测均阴性。4株诱导后对亚胺培南耐药的菌株中,3株(H099、R010及T375)blaAdeB表达量增加2~14倍。另外1株K645菌株blaAdeB表达量无增加,blaOXA-51-like表达量增加17倍。2株诱导后亚胺培南MIC值无升高的菌株,其诱导前后blaAdeB及blaOXA-51-like表达量均无增加。结论在对鲍曼不动杆菌临床治疗应当尽量避免亚MIC浓度碳青霉烯类抗菌药物暴露,避免细菌耐药表型变化。 AdeABC外排系统的过表达可能同本实验1株鲍曼不动杆菌对亚胺培南获得性耐药相关。 Objective To evaluate the mechanism of AdeABC efflux pump on induced imipenem resistant Acinetobacter baumannii clinical iso-lates.Methods Six imipenem susceptible A.baumannii isolates were induced by broth dilution method.MICs of 10 antibiotics with carbonyl cyanidem chlorophenylhydrazone ( CCCP ) were tested by agar dilution.The isolates were genotyped by pulsed -field gel electrophoresis ( PF-GE).PCR assays for genes coding for related genes were performed.The levels of blaAdeB and blaOXA-51-like mRNA expressions were tested by real -time PCR assays.Results The six imipenem susceptible A.baumannii isolates were all multidrug resistant.Four isolates exhibited imipenem re-sistantance after induced by imipenem , and MICs of imipenem and mero-penem with CCCP were two to eight times lower.Nucleotide sequencing showed that the blaOXA-66 alleles were detected.PCR for tested genes was negative except the blaOXA-51-like.The levels of blaAdeB mRNA expression in 3 induced imipenem resistant isoaltes increased two to fourteen times than the original isolates , while the isolate ( K645 ) with 17 times in-crease of the expression of blaOXA-51-like mRNA did not show increasing expression of blaAdeB mRNA.Two isolates were still imipenem susceptible after induced by imipenem which expression levels of blaAdeB and blaOXA-51-like mRNA showed no increasing.Conclusion The patients with A.baumannii infection should avoid being exposed to the subinhibitory concentration of carbapenem , to prevent changes of bacterial resistant profiles.AdeABC efflux pump probably contributes to the resistance of imipenem in one tested A.baumannii isolate.
语种: 中文
原文出处: 查看原文
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内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/44469
Appears in Collections:北京大学第一临床医学院_临床药理研究所_期刊论文

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作者单位: 北京大学 第一医院 临床药理研究所,北京,100034

Recommended Citation:
马序竹,李湘燕,薛峰,等. 亚胺培南诱导耐药鲍曼不动杆菌adeB表达变化研究[J]. 中国临床药理学杂志,2014(8):696-700.
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