IR@PKUHSC  > 北京大学第一临床医学院  > 临床药理研究所
学科主题药理学
β内酰胺类/β内酰胺酶抑制剂的体外抗菌活性研究
其他题名Antibacterial activity study of several β-lactam/β-lactamase inhibitor combinations in vitro
欧尾妹; 吕媛; 李耘; 薛峰; 刘健; 杨维维; 张佳
关键词Β内酰胺类/β内酰胺酶抑制剂 最低抑菌浓度 细菌耐药 超广谱β内酰胺酶 Β-lactam/β-lactam Inhibitor Combinations Minimum Inhibitory Concentration Resistance Extended-spectrumβ-lactamase
刊名中国临床药理学杂志
2015
DOI10.13699/j.cnki.1001-6821.2015.11.006
11页:916-920
收录类别中国科技核心期刊 ; 中文核心期刊 ; CSCD
文章类型Journal Article
摘要目的:比较不同β内酰胺类/β内酰胺酶抑制剂对肠杆菌科细菌的体外抗菌活性。方法收集临床分离肠杆菌科细菌,用平皿二倍稀释法测定抗菌药最低抑菌浓度( MIC),根据2014版美国临床实验室标准化委员会( CLSI)折点判读结果,用SPSS17.0统计软件处理数据。结果共收集到2166株肠杆菌科细菌。哌拉西林对大肠埃希菌的MIC50和MIC90分别是256,512μg· mL-1,加用他唑巴坦后MIC50和MIC90则分别为2,8μg· mL-1。对产超广谱β内酰胺酶( ESBLs)大肠埃希菌和肺炎克雷伯菌,哌拉西林/他唑巴坦敏感率最高为93.7%和86.1%,头孢哌酮/他唑巴坦为91.59%和82.59%,头孢哌酮/舒巴坦为74.4%和58.37%。复方制剂对非产ESBLs大肠埃希菌和肺炎克雷伯菌的敏感率与单用β内酰胺类相差不大。β内酰胺类抗菌药对普通变形杆菌的敏感率比奇异变形杆菌高,但加用β内酰胺酶抑制剂后两者敏感率差不多。美洛西林/舒巴坦对摩根菌属敏感率为97.4%,明显高于单用美洛西林的76.3%。头孢噻肟/他唑巴坦和头孢曲松/他唑巴坦对肠杆菌科细菌的MIC90比单用头孢噻肟和头孢曲松低。结论β内酰胺类/β内酰胺酶抑制剂对肠杆菌科细菌的抗菌活性比单用β内酰胺类抗菌药强,尤其对产ESBLs大肠埃希菌和肺炎克雷伯菌差异更为明显,其中以哌拉西林/他唑巴坦的抗菌活性最强。 Objective To evaluate the antibacterial activities of severalβ-lactam/β-lactam inhibitor combinations in vitro.Methods All the clinical isolates were Enterobacteriaceae collected from hospitals and the susceptibility tests were performed using agar dilution method recom-mended by CLSI.The minimum inhibitory concentration ( MIC ) results were interpreted according to the breakpoints of CLSI (2014) guideline.The data was analyzed by software SPSS17.0.Results A total of 2166 Enterobacteriaceae were collected and were performed MIC tests.Based on the MIC results, the MIC50 and MIC90 of piperacillin to Escherichia coli were 256 , 512 μg · mL-1 , while piperacillin/tazobactam were 2 , 8μg· mL-1 , respectively.The sensitive rates of piperacillin/tazobactam to ESBLs -producing Escherichia coli and Klebsiella pneumoniae were 93.7% and 86.1%, cefoperazone/tazobactam were 91.5% and 82.5%, while cefoperazone /sulbactam were 74.4% and 58.39%. Among ESBLs -non -producing Escherichia coli and Klebsiella pneumoniae, there is not much difference between using β-lactam/β-lactam inhibitor combinations and β-lactams alone.β-lactam antibiotics have higher sensitive rates to Proteus vulgaris than Proteus mirabilis, butβ-lactam/β-lactam inhibitor combinations have no difference against them.The sensitive rate of mezlocillin/sulbactam to Morganella spp.was 97.4%, significantly higher than mezlocillin alone with sensitive rate 76.3%.The MIC90 of cefotaxime/tazobactam and ceftriaxone/tazobactam were lower than using cefotaxime and ceftriaxone alone.Conclusion β-lactam/β-lactam inhibitor combinations have greater antimicrobial activity to Enterobacteriaceae than β-lactams alone, especially for ESBLs-producing Escherichia coli and Klebsiella pneumoniae.Among them, piperacillin/tazobactam has the greatest antimicrobial activity.
语种中文
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文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/44478
专题北京大学第一临床医学院_临床药理研究所
作者单位北京大学第一医院 临床药理研究所,北京,100191
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GB/T 7714
欧尾妹,吕媛,李耘,等. β内酰胺类/β内酰胺酶抑制剂的体外抗菌活性研究[J]. 中国临床药理学杂志,2015(11):916-920.
APA 欧尾妹.,吕媛.,李耘.,薛峰.,刘健.,...&张佳.(2015).β内酰胺类/β内酰胺酶抑制剂的体外抗菌活性研究.中国临床药理学杂志(11),916-920.
MLA 欧尾妹,et al."β内酰胺类/β内酰胺酶抑制剂的体外抗菌活性研究".中国临床药理学杂志 .11(2015):916-920.
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