北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第一临床医学院  > 临床药理研究所  > 期刊论文
学科主题: 药理学
题名:
分子生物学方法在肠球菌耐药性研究中的应用
其他题名: Application of Molecular Biology in Investigation of Resistance in Enterococci
作者: 王珊; 吕媛
关键词: 肠球菌 ; 耐药性 ; 分子生物学 ; 万古霉素耐药肠球菌 ; enterococci ; resistance ; molecular biology ; vancomycin-resistant enterococci
刊名: 现代检验医学杂志
发表日期: 2015
DOI: 10.3969/j.issn.1671-7414.2015.01.002
期: 1, 页:4-7
收录类别: 中国科技核心期刊
文章类型: Journal Article
摘要: 目的:为了及时准确地检测细菌的耐药性,该文将肠球菌耐药性研究中的分子生物学方法进行综述。方法对近年来肠球菌耐药性研究的相关文献进行整理、分析与归纳。结果一些方法比较常见,如 PCR,PFGE,MSLT和 Southern杂交等,而一些方法如焦磷酸测序和基因芯片技术,目前在细菌耐药机制研究中的应用并不广泛,属于交叉学科,但是稍加利用会有一定的发展前景。结论任何一个方法都有优点,也存在一些局限性,应取长补短,发挥每种方法的最大优势。 Objective This article aims to provide mechanisms and recent developments of molecular biology pertaining to re-sistance of Enterococci,providing rapid approaches for detecting resistant strains.Methods This article reviewed recent lit-eratures on resistance of Enterococci and a systemic analysis was conducted.Results Common detecting methods include polymerase chain reaction (PCR),pulsed field gel electrophoresis (PFGE),multilocus sequence typing (MLST)and South-ern blot.There also exist less widely-used methods such as pyrosequencing and genechip technique,which may prove effi-cient in some aspects.Conclusion Every method has its advantages and disadvantages.This article discussed how to utilize these methods to achieve their maximum capabilities.
语种: 中文
原文出处: 查看原文
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/44552
Appears in Collections:北京大学第一临床医学院_临床药理研究所_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 北京大学第一医院临床药理研究所,北京,100191

Recommended Citation:
王珊,吕媛. 分子生物学方法在肠球菌耐药性研究中的应用[J]. 现代检验医学杂志,2015(1):4-7.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[王珊]'s Articles
[吕媛]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[王珊]‘s Articles
[吕媛]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace