IR@PKUHSC  > 北京大学第一临床医学院  > 临床药理研究所
学科主题药理学
我国阿米卡星耐药大肠埃希菌16SrRNA甲基化酶基因及其水平转移研究
其他题名Study on the 16S rRNA methylase genes in amikacin resistant Escherichia coli and their horizontal transfer in China
王珊; 吕媛; 李耘; 薛峰; 刘健
关键词阿米卡星 大肠埃希菌 16srrna甲基化酶基因 基因水平转移 Amikacin Escherichia Coli 16s Rrna Methylase Gene Horizontal Gene Transfer
刊名中国临床药理学杂志
2015
DOI10.13699/j.cnki.1001-6821.2015.04.014
4页:283-285
收录类别中国科技核心期刊 ; 中文核心期刊 ; CSCD
文章类型Journal Article
摘要目的:研究我国临床分离阿米卡星耐药大肠埃希菌中16S rRNA甲基化酶基因及其水平转移情况。方法用PCR法检测16S rRNA甲基化酶基因(armA、rmtA、rmtB、rmtC、rmtD、rmtE和npmA),用液体培养基传递法研究其水平转移情况。结果183株对阿米卡星耐药大肠埃希菌中,armA基因阳性率为12.57%, rmtB基因阳性率为53.00%,rmtB基因阳性率在2007至2008年、2009至2010年、2011至2012年这3个年度之间比较差异有统计学意义。 armA 和rmtB基因均可在大肠埃希菌间水平转移。结论16SrRNA甲基化酶基因以armA和rmtB为主要流行型类别,后者的分离率较高且其阳性率呈显著的上升趋势。 Objective To investigate 16S ribosome ribonucleic acid ( rRNA) methylase genes in clinically isolated amikacin-resistant Esche-richia coli ( E.coli ) and their horizontal transfer in China. Methods Polymerase chain reaction ( PCR ) was used to detect 16S rRNA methylase genes ( armA、rmtA、rmtB、rmtC、rmtD、rmtE and npmA) and liquid transfer employed to study horizontal transfer of these methy-lase genes.Results Of 183 amikacin-resistant strains of E.coli, those with armA and rmtB accounted for 12.57%and 53.00%, respectively. Prevalences of rmtB were found significantly different among three time points from 2007 to 2012.Both armA and rmtB are found to transfer among amikacin-resistant E.coli in a horizontal way.Conclusion The 16 S rRNA methylase genes of armA and rmtB are main types in amikacin-resistant E.coli in China, the latter with predominant isolation rate and increasing positive rate.
语种中文
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文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/44553
Collection北京大学第一临床医学院_临床药理研究所
作者单位北京大学 第一医院 临床药理研究所,北京,100034
Recommended Citation
GB/T 7714
王珊,吕媛,李耘,等. 我国阿米卡星耐药大肠埃希菌16SrRNA甲基化酶基因及其水平转移研究[J]. 中国临床药理学杂志,2015(4):283-285.
APA 王珊,吕媛,李耘,薛峰,&刘健.(2015).我国阿米卡星耐药大肠埃希菌16SrRNA甲基化酶基因及其水平转移研究.中国临床药理学杂志(4),283-285.
MLA 王珊,et al."我国阿米卡星耐药大肠埃希菌16SrRNA甲基化酶基因及其水平转移研究".中国临床药理学杂志 .4(2015):283-285.
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