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学科主题: 心血管病学
题名:
尾加压素Ⅱ对大鼠主动脉一氧化氮合酶/一氧化氮系统的影响
其他题名: Effect of urotensin Ⅱ on nitric oxide synthese/nitric oxide pathway in rat aorta
作者: 姜志胜1; 张勇刚1; 齐永芬1; 许松1; 徐少平1; 庞永政1; 唐朝枢1
关键词: 尾加压素Ⅱ ; 一氧化氮合酶 ; 一氧化氮/代谢 ; 血管升压素/药理学 ; 主动脉/化学
刊名: 北京大学学报(医学版)
发表日期: 2001
DOI: 10.3321/j.issn:1671-167X.2001.02.013
卷: 33, 期:2, 页:147-149
文章类型: Journal Article
摘要: 目的:研究尾加压素Ⅱ(urotensin Ⅱ, U Ⅱ)对大鼠主动脉一氧化氮合酶(nitric oxide synthase, NOS)/一氧化氮(nitric oxide, NO)系统的影响。方法:体外血管薄片孵育,以生化及放射活性分析方法分别测定孵育液中亚硝酸盐(NO-2)含量, 血管 NOS活性及cGMP含量。结果:孵育1.5 h到3 h,U Ⅱ(10-9~10-7 mol*L-1)呈浓度依赖地刺激血管NO-2生成增加(14.8%~80.9%)。 U Ⅱ刺激血管组织总NOS和固有型NOS活性显著增加(P<0.05或P<0.01)而不影响iNOS 活性(P>0.05)。10-9~10-7 mol*L-1的U Ⅱ呈浓度依赖的增加血管组织cGMP含量(P<0.01)。实验还观察到NOS抑制剂左旋硝基精氨酸(GN-L-nitro-arginine, L-NNA)显著抑制了U Ⅱ刺激的血管组织NOS激活、NO生成和cGMP含量增加。结论:U Ⅱ激活血管组织NOS/NO途径,增加血管NO生成与cGMP含量。 Objective: To study the effect of urotensin(U Ⅱ) on nitric oxide synthase(NOS)/nitric oxide(NO) system in rat aorta. Methods: The aortic slices were incubated in vitro. The medium nitrite content, vascular NOS activities and cGMP content were measured. Results: After incubation of aorta for 1.5-3.0 h, UⅡ(10-9-10-7 mol*L-1) increased nitrite production ( 14.8%-80.9%, P<0.01) in dose-dependent manner. U Ⅱ significantly stimulated the total NOS activities, mainly activated constitutive NOS (P<0.05 or P<0.01), however, the inducible NOS activities were not altered (P>0.05). Incubation of aortic slices with U Ⅱ (10-9-10-7 mol*L-1) increased vascular cGMP content in dose-dependent manner. Co-incubation vascular tissue with U Ⅱ and GN-L-nitro-arginine, an inhibitor of NOS, obviously inhibited the U Ⅱ-induced NOS activation, NO production and cGMP formation. Conclusion: The results suggest that U Ⅱ can activate the vascular NOS/NO pathway, increasing tissue NO production and cGMP content.
语种: 中文
原文出处: 查看原文
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内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/44774
Appears in Collections:北京大学第一临床医学院_心血管内科_期刊论文

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作者单位: 1.北京大学第一医院心血管病研究所,北京 100034
2.北京大学第一医院中心实验室,北京 100034
3.中国人民解放军北京军区总医院心内科

Recommended Citation:
姜志胜,张勇刚,齐永芬,等. 尾加压素Ⅱ对大鼠主动脉一氧化氮合酶/一氧化氮系统的影响[J]. 北京大学学报(医学版),2001,33(2):147-149.
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