IR@PKUHSC  > 北京大学第一临床医学院  > 心血管内科
学科主题心血管病学
自发性高血压大鼠和Wistar-Kyoto大鼠心血管组织肾上腺髓质素和肾上腺髓质素原N-末端20肽的水平
其他题名Levels of adrenomedullin and proadrenomedullin N-terminal 20 peptide in myocardium and aorta of spontaneously hypertensive rats and Wistar-Kyoto rats
齐永芬1; 卜定方1; 石彦荣1; 李菊香1; 庞永正1; 唐朝枢1
关键词病理生理学 肾上腺髓质素 肾上腺髓质素原n末端20肽 自发性高血压大鼠 Mrna Pathophysiology Adrenomedullin Proadrenomedullin n Terminal 20 Peptide (Pamp) Spontaneously Hypertensive Rat Mrna
刊名生理学报
2003
DOI10.3321/j.issn:0371-0874.2003.03.005
55期:3页:260-264
收录类别中国科技核心期刊 ; 中文核心期刊 ; CSCD
文章类型Journal Article
摘要本工作观察了自发性高血压大鼠(SHRs)和Wistar-kyoto (WKY)大鼠心肌和主动脉肾上腺髓质素(adrenomedullin, ADM)和肾上腺髓质素原N-末端20肽(proadrenomedullin N terminal 20 peptide, PAMP)的水平.以放射免疫分析方法测定血浆、心肌和主动脉ADM含量.用竞争性定量逆转录多聚酶链式反应(RT-PCR)方法测定心肌和主动脉ProADM mRNA含量.结果发现, SHRs心肌和主动脉ProADM mRNA水平分别比WKY大鼠高66.7% 和73%(均P<0.01).SHRs血浆、心肌和主动脉ADM-ir含量分别较WKY大鼠高29%、76.7%和79%(均P<0.01).SHRs血浆、心肌和主动脉PAMP-ir水平分别较WKY大鼠高42.5%(P<0.01)、47.2%(P<0.01)和27.3%(P<0.05).另外, SHRs 的ADM 和PAMP的比值较WKY大鼠明显增高(心肌和主动脉分别为2.0±0.25 vs 1.64±0.3和 2.2±0.18 vs 1.56±0.28).结果提示, SHRs心肌和主动脉ProADM基因表达上调, ADM和PAMP水平升高, 但二者升高的比例不一致.SHRs 的ADM和PAMP升高不一致的病理生理意义有待进一步研究. In this study, we observed the levels of adrenomedullin (ADM) and proadrenomedullin N-terminal 20 peptide (PAMP) in myocardium and aorta of spontaneously hypertensive rats (SHRs) in comparison with Wistar-kyoto (WKY) rats. Contents of ADM and PAMP were measured by radioimmunoassay (RIA) in plasma, myocardium and aorta. The amount of Pro-ADM mRNA of myocardium and aorta was determined by competitive quantitative reverse transcription polymerase chain reaction (RT-PCR). In SHRs the amounts of Pro-ADM mRNA of myocardium and aorta were 66.7% (P<0.01) and 73% (P<0.01) higher than those in WKY rat, respectively. In SHRs, the levels of ADM in plasma, myocardium and aorta were 29%, 76.7% and 79% (all P<0.01) higher than those in WKY rats, respectively. The level of PAMP in SHRs was increased by 42.5% in plasma (P<0.01), 47.2% in myocardium (P<0.0.1) and 27.3% in aorta (P<0.05) compared to WKY rats, respectively. In addition, the ratio of ADM content to PAMP content in SHRs group was increased compared with that in WKY group (2.0±0.25 vs 1.64±0.3 and 2.2±0.18 vs 1.56±0.28, in myocardium and aorta, respectively, P<0.01). These results suggest that ProADM gene expression is up-regulated and the increase in ADM and PAMP is different in SHRs. The significance of inconsistency of increase in ADM and PAMP in SHRs needs to be further investigated.
语种中文
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文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/44806
专题北京大学第一临床医学院_心血管内科
作者单位1.北京大学第一医院心血管病研究所,北京,100034
2.北京大学医学部生理学系,北京,100083
3.北京大学第一医院心血管病研究所,北京,100034
4.北京大学医学部生理学系,北京,100083
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齐永芬,卜定方,石彦荣,等. 自发性高血压大鼠和Wistar-Kyoto大鼠心血管组织肾上腺髓质素和肾上腺髓质素原N-末端20肽的水平[J]. 生理学报,2003,55(3):260-264.
APA 齐永芬,卜定方,石彦荣,李菊香,庞永正,&唐朝枢.(2003).自发性高血压大鼠和Wistar-Kyoto大鼠心血管组织肾上腺髓质素和肾上腺髓质素原N-末端20肽的水平.生理学报,55(3),260-264.
MLA 齐永芬,et al."自发性高血压大鼠和Wistar-Kyoto大鼠心血管组织肾上腺髓质素和肾上腺髓质素原N-末端20肽的水平".生理学报 55.3(2003):260-264.
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