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学科主题: 心血管病学
题名:
尾加压素Ⅱ对血管平滑肌细胞增殖的影响
其他题名: Stimulating proliferation of aorta smooth muscle cells of rat by rat urotensin Ⅱ
作者: 张勇刚; 齐永芬; 夏春芳; 庞永正; 杨军; 张肇康; 唐朝枢
关键词: 尾加压素Ⅱ ; 细胞增生 ; 有丝分裂原
刊名: 中国药理学通报
发表日期: 2001
DOI: 10.3321/j.issn:1001-1978.2001.02.009
卷: 17, 期:2, 页:155-157
收录类别: 中国科技核心期刊 ; 中文核心期刊 ; CSCD
文章类型: Journal Article
摘要: 目的研究体内新发现的缩血管活性肽尾加压素Ⅱ(urotensin Ⅱ,UⅡ)对大鼠主动脉平滑肌细胞增殖作用的影响以及UⅡ作用的细胞内信号转导机制。方法在培养的大鼠主动脉平滑肌细胞(ASMC)上,采用[3H]-胸腺嘧啶([3H]-TdR)参入法,观察UⅡ对细胞DNA合成的刺激作用;加入不同的细胞内信号转导阻断剂,观察对UⅡ效应的影响。结果 1×10-9~1×10-7 m ol*L-1 UⅡ以浓度依赖方式促进ASMC [3H]-TdR参入增加,1×10-9 、1 ×10-8和1×10-7 mol*L-1 UⅡ组[3H]-TdR参入量分别较对照组增加22%(P<0.05),57%(P<0.01)和65%(P<0.01)。UⅡ的效应能被钙通道阻断剂尼卡地平、PKC阻断剂H7、钙调素激酶(CaM-PK)阻断剂W7和MAPK阻断剂P D98059所阻断,抑制率分别为55%(P<0.01),27%(P<0.01),18%(P <0.05)和16%(P<0.05)。结论 UⅡ是一种新发现的强烈的促AS MC增生的内源性丝裂原,其促丝裂效应可能通过Ca2+、PKC、CaM-PK和MAPK来介导。 AIM To investigate effect of urotensin Ⅱ (UⅡ)on proliferation of aort a smooth muscle cells (ASMC)of rat and study the signal transduction pathway o f it. METHODS In cultured ASMC of rat, UⅡ was used to stimulate proliferation of these cells and levels of [3H]-TdR incorporation were used to evaluate the speed of DNA synthesis, and different inhibitors were used to s tudy the action of different signal transduction pathway of mitogenic effect of UⅡ on VSMC. RESULTS 1×10-9~1×10-7 mol*L- 1 UⅡ caused marked concentration-dependent increasing of [3H]-TdR i ncor poration of ASMC. [3H]-TdR incorporation of 1×10-9,1×10-8 and 1×10-7 mol*L-1 UⅡ were 22%(P<0.05), 57%(P<0.01)and 65%(P<0.01)higher than control. Nicardipine, H7, W7 and PD98059 , which are inhibitors of calcium channel, PKC, CaM-PK and MAPK respectively, inhibited the effects of UⅡ obviously, with the inhibitory rate by 55%(P< 0.01), 27%(P<0.01),18%(P<0.05)and 16%(P<0.05)respectively . CONCLUSION UⅡ is a strong mitogen for VSMC and the mitogenic e ffect of UⅡ is probably mediated by Ca2+, PKC, CaM-PK and MAPK signal tr ansduction pathway.
语种: 中文
原文出处: 查看原文
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内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/44873
Appears in Collections:北京大学第一临床医学院_心血管内科_期刊论文

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作者单位: 北京大学第一医院心血管病研究所,

Recommended Citation:
张勇刚,齐永芬,夏春芳,等. 尾加压素Ⅱ对血管平滑肌细胞增殖的影响[J]. 中国药理学通报,2001,17(2):155-157.
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