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学科主题: 中西医结合
题名:
加味五子衍宗方总黄酮对大鼠海马锥体细胞VGCC通道的影响
其他题名: Effect of total flavonoids of Jiawei Wuzi Yanzong prescription on VGCCs induced by amyloid-β_(25-35) peptide in CA1 pyramidal neurons of rat hippocampal slice
作者: 李娌1; 蔡浩然1; 李琳1; 王学美1
关键词: β淀粉样蛋白 ; 锥体细胞 ; 全细胞膜片钳记录 ; 五子衍宗方 ; 电压依赖钙通道 ; Aβ_(25-35) ; pyramidal cells ; whole-cell patch-clamp ; Wuzi Yanzong prescription ; voltage-gated Ca~(2+) channels (VGCCs)
刊名: 中国中药杂志
发表日期: 2009
卷: 34, 期:15, 页:1975-1978
收录类别: 中国科技核心期刊 ; 中文核心期刊 ; CSCD
文章类型: Journal Article
摘要: 目的:探讨加味五子衍宗方总黄酮成分对Aβ_(25-35)作用后的大鼠海马CA1区锥体神经元电压门控钙通道电流的作用.方法:应用全细胞膜片钳技术记录海马神经元I_(Ca)电流,采用内向电流的幅度作为观察指标,观察模型药物及干预药物对其的作用.采用出生7 d的乳鼠取海马后制成脑片,选取立体感强、状态好的锥体细胞形成全细胞膜片钳记录,从-50 mV到+50 mV范围(步进为5 mV)一系列去极化电压脉冲刺激(脉冲宽度500 ms),以激发出电压依赖性的内向钙离子电流.记录电流后加入Aβ_(25-35)后,再加入总黄酮进行干预.结果:Aβ_(25-35)淀粉蛋白可以通过增强I_(Ca)可引起胞内钙超载,正常幅度平均为-(157.1±19.9)pA,Aβ_(25-35)作用后增大为-(323.2±23.4)pA,125,250,500 mg·L~(-1)的总黄酮作用之后,分别下降为-(257.9±31.6),-(196.4±29.8),-(169.3±34.0)pA.结论:引起胞内钙离子超载可能是Aβ_(25-35)产生神经毒性作用的机制之一,加味五子衍宗方总黄酮成分可通过抑制钙离子内流对神经元起一定保护作用. Objective:To explore the effect of the total flavonoids of Jiawei Wuzi Yanzong prescription on the Voltage-gated calcium channel of the CA1 pyramidal cell of rat hippocampus.Method:The inward calcium current was recorded by the whole-cell patch clamp and the anaplitude of it was thought to observe the effect of Aβ_(25-35) and the total flavonoids.The hippoeampus of rat was separated and cut into slices.Active pyramidal cells of slices were chosen for the whole-cell patch clamp recording.After exposure to Aβ_(25-35),voltage steps(500 ms)were used to depolarize stepwise in a range from 50 to +50 mV(increment:5 mV).An inward Ca~(2+) current which was suggested to be survey was evoked.Application of the total flavonoids was to be observed if it had effect on this voltage-depended inward current.Result:Aβ_(25-35) could enhance the calcium current to induce intracellular calcium overload.The amplitude of the control group was -(157.1±19.9) pA,but after application of Aβ_(25-35) the current enhanced to -(323.2±23.4)pA.When the total flavonoids at concentration of 125,250,500 g·L~(-1) were added,the current declined to -(257.9±31.6),-(196.4±29.8) and -(169.3±34.0) pA,respectively.Conclusion:Aβ-induced intracellular calcium overload may be one of neurotoxic of β-amyloid peptide.The total flavonoids of Jiawei Wuzi Yanzong prescription can suppress inward calcium current to protect neurons.
语种: 中文
原文出处: 查看原文
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内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/44902
Appears in Collections:北京大学第一临床医学院_中医中西医结合科_期刊论文

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作者单位: 1.北京大学,第一医院中西医结合研究室,北京,100034
2.北京大学,第一医院,中心实验室,北京,100034

Recommended Citation:
李娌,蔡浩然,李琳,等. 加味五子衍宗方总黄酮对大鼠海马锥体细胞VGCC通道的影响[J]. 中国中药杂志,2009,34(15):1975-1978.
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