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学科主题: 泌尿外科学
题名:
结石模型大鼠肾脏骨桥蛋白的表达调控
其他题名: Regulation of osteopontin expression in a rat model of urolithiasis
作者: 常连胜1; 冯陶1; 魏今1; 李菊香1
关键词: 尿石症 ; 动物模型 ; 骨桥蛋白 ; 表达 ; urolithiasis ; animal model ; osteopontin ; regulation
刊名: 中华医学杂志(英文版)
发表日期: 2001
DOI: 10.3760/j.issn:0366-6999.2001.08.011
卷: 114, 期:8, 页:829-832
文章类型: Journal Article
摘要: 目的探讨骨桥蛋白(OPN)的表达和调控与尿石症的关系。 方法分别用维生素D、维生素K、雌二醇和睾酮处理正常大鼠和结石模型大鼠,连续用药7天,免疫组化和原位杂交技术检测大鼠肾脏OPN及其mRNA的表达,偏光显微镜观察鼠肾草酸钙晶体沉积情况,并测定尿晶体成分的浓度。 结果维生素K、雌二醇和睾酮均可上调OPN及其mRNA的表达,减少鼠肾草酸钙晶体的沉积。维生素D增加尿钙的排泄量,促进晶体在鼠肾的沉积。 结论结果提示,维生素K、雌二醇和睾酮可上调OPN的表达,抑制结石的形成,维生素D过量则可促进结石形成。 Objective To investigate the relationship between the expression and regulation of osteopontin (OPN) and urolithiasis. Methods Normal and stone model rats were treated with 1,25-dihydroxyvitamin D3 (D3), vitamin K, testosterone or estradiol for 7 days, and the expression of osteopontin and its mRNA were detected with immunohistochemistry and Northern blot, respectively. Crystals deposited in rat kidneys were observed with a polarization microscope. The concentrations of crystal components in rat urine were determined. Results The results showed that vitamin K, testosterone and estradiol up-regulated the expression of OPN mRNA and its protein, thus decreasing the precipitation of calcium oxalate in rat kidneys. D3 increased the concentration of calcium in urine, and accelerated the sedimentation of calcium oxalate in rat kidneys. Conclusions These findings indicate that OPN may be an important macromolecule in the normal endogenous inhibition of the formation of urolithiasis. Vitamin K, testosterone and estradiol inhibit the formation of stones via up-regulating the expression of OPN in kidneys, while D3 over dose may accelerate the process.
语种: 中文
原文出处: 查看原文
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内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/45007
Appears in Collections:北京大学第一临床医学院_泌尿外科_期刊论文

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作者单位: 1.北京大学第一医院泌尿外科研究所北京 100034
2.北京大学生理系北京 100083

Recommended Citation:
常连胜,冯陶,魏今,等. 结石模型大鼠肾脏骨桥蛋白的表达调控[J]. 中华医学杂志(英文版),2001,114(8):829-832.
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