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核小体结合蛋白1在雄激素非依赖性前列腺癌中的作用
其他题名Effect of nucleosomal binding protein 1 in androgen-independent prostatic carcinoma
黄晨; 周利群; 宋刚
关键词前列腺肿瘤 Rna 核小体结合蛋白1 Prostatic Neoplasms Rna Nucleosomal Binding Protein 1
刊名中华医学杂志
2008
DOI10.3321/j.issn:0376-2491.2008.10.003
88期:10页:657-660
收录类别中国科技核心期刊 ; 中文核心期刊 ; CSCD
文章类型Journal Article
摘要目的 明确核小体结合蛋白1(NSBP1)在雄激素非依赖性前列腺癌细胞(PC-3)中的生物学功能.方法 通过RNA干扰技术,使PC-3细胞中的NSBP1表达水平明显降低,然后用胆囊收缩素(CCK)-8法观察细胞活性的变化,用流式细胞光度术检测细胞周期的变化.结果 NSBP 1的表达抑制后,从60 h开始PC-3 细胞活性(A值为0.329±0.017与0±386±0.022)有显著下降(P<0.05),抑制率到108 h达到高峰(27.5%).流式细胞光度术检测结果显示,NSBP1抑制后的PC-3细胞的G2M+S期细胞百分率也从60 h开始(31.7%±2.0%)与对照组(37.3%±2.5%)有统计学意义(P<0.05).结论 NSBP 1在前列腺癌PC-3细胞中,对维持细胞活性及促进细胞生长增殖有重要作用. Objective To investigate biological function of nucleosomal binding protein 1(NSBP1)in androgen-independent prostate cancer cells. Methods Androgen-independent prostate cancer cells of the line PC-3 were cultured and transfected with recombinant inhibiting siRNA expression plasmid pSilencer 2.1-U6 neo.Before transfection and 12,36,60,84,108 and 132 h after transfection RT-PCR and Western blotting was used to detect the inhibition effect. CCK-8 was used to detect the viability of the cells.The cell cycle was examined by flow cytometry. Results The cell viability was significantly reduced since 60-108 h after the transfection(all P<0.05),the inhibition ratio reaching climax 108 hours after the transfection(P=0.028).However,the inhibition ratio began to increase.FC showed that since 60 h after the transfection the proportion of PC-3 cells at the G2M+S phase began to increased compared to that of the control group ( all P<0.05).Conclusion NSBP1 has important effect on maintaining cell viability and enhancing growth and proliferation of androgen-independent prostate cancer cells.
语种中文
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文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/45157
Collection北京大学第一临床医学院_泌尿外科
作者单位北京大学泌尿外科研究所,北京大学第一医院泌尿外科,100034
Recommended Citation
GB/T 7714
黄晨,周利群,宋刚. 核小体结合蛋白1在雄激素非依赖性前列腺癌中的作用[J]. 中华医学杂志,2008,88(10):657-660.
APA 黄晨,周利群,&宋刚.(2008).核小体结合蛋白1在雄激素非依赖性前列腺癌中的作用.中华医学杂志,88(10),657-660.
MLA 黄晨,et al."核小体结合蛋白1在雄激素非依赖性前列腺癌中的作用".中华医学杂志 88.10(2008):657-660.
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