北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第一临床医学院  > 泌尿外科  > 期刊论文
学科主题: 泌尿外科学
题名:
核小体结合蛋白1在雄激素非依赖性前列腺癌中的作用
其他题名: Effect of nucleosomal binding protein 1 in androgen-independent prostatic carcinoma
作者: 黄晨; 周利群; 宋刚
关键词: 前列腺肿瘤 ; RNA ; 核小体结合蛋白1 ; Prostatic neoplasms ; RNA ; Nucleosomal binding protein 1
刊名: 中华医学杂志
发表日期: 2008
DOI: 10.3321/j.issn:0376-2491.2008.10.003
卷: 88, 期:10, 页:657-660
收录类别: 中国科技核心期刊 ; 中文核心期刊 ; CSCD
文章类型: Journal Article
摘要: 目的 明确核小体结合蛋白1(NSBP1)在雄激素非依赖性前列腺癌细胞(PC-3)中的生物学功能.方法 通过RNA干扰技术,使PC-3细胞中的NSBP1表达水平明显降低,然后用胆囊收缩素(CCK)-8法观察细胞活性的变化,用流式细胞光度术检测细胞周期的变化.结果 NSBP 1的表达抑制后,从60 h开始PC-3 细胞活性(A值为0.329±0.017与0±386±0.022)有显著下降(P<0.05),抑制率到108 h达到高峰(27.5%).流式细胞光度术检测结果显示,NSBP1抑制后的PC-3细胞的G2M+S期细胞百分率也从60 h开始(31.7%±2.0%)与对照组(37.3%±2.5%)有统计学意义(P<0.05).结论 NSBP 1在前列腺癌PC-3细胞中,对维持细胞活性及促进细胞生长增殖有重要作用. Objective To investigate biological function of nucleosomal binding protein 1(NSBP1)in androgen-independent prostate cancer cells. Methods Androgen-independent prostate cancer cells of the line PC-3 were cultured and transfected with recombinant inhibiting siRNA expression plasmid pSilencer 2.1-U6 neo.Before transfection and 12,36,60,84,108 and 132 h after transfection RT-PCR and Western blotting was used to detect the inhibition effect. CCK-8 was used to detect the viability of the cells.The cell cycle was examined by flow cytometry. Results The cell viability was significantly reduced since 60-108 h after the transfection(all P<0.05),the inhibition ratio reaching climax 108 hours after the transfection(P=0.028).However,the inhibition ratio began to increase.FC showed that since 60 h after the transfection the proportion of PC-3 cells at the G2M+S phase began to increased compared to that of the control group ( all P<0.05).Conclusion NSBP1 has important effect on maintaining cell viability and enhancing growth and proliferation of androgen-independent prostate cancer cells.
语种: 中文
原文出处: 查看原文
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/45157
Appears in Collections:北京大学第一临床医学院_泌尿外科_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 北京大学泌尿外科研究所,北京大学第一医院泌尿外科,100034

Recommended Citation:
黄晨,周利群,宋刚. 核小体结合蛋白1在雄激素非依赖性前列腺癌中的作用[J]. 中华医学杂志,2008,88(10):657-660.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[黄晨]'s Articles
[周利群]'s Articles
[宋刚]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[黄晨]‘s Articles
[周利群]‘s Articles
[宋刚]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace