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学科主题: 泌尿外科学
题名:
Bcl-2和Bax在前列腺癌不同周期时相的表达
其他题名: Differential transcription of Bcl-2 and Bax through the cell cycle in prostate cancer cell line
作者: 李学松1; 龚侃1; 孙国锋1; 周利群1; 何志嵩1; 辛殿琪1; 那彦群1; 梁云燕1; 王代树1; 郭应禄1
关键词: 前列腺肿瘤 ; 细胞周期 ; 凋亡 ; Bcl-2 ; Prostate neoplasms ; Cell cycle ; Apoptosis ; Bcl-2
刊名: 中华外科杂志
发表日期: 2008
DOI: 10.3321/j.issn:0529-5815.2008.10.013
卷: 46, 期:10, 页:768-771
收录类别: 中国科技核心期刊 ; 中文核心期刊 ; CSCD
文章类型: Journal Article
摘要: 目的 探讨凋亡相关基因Bel-2和Bax在前列腺癌不同周期时相的差异表达及意义.方法 应用改良的胸腺嘧啶核苷和高压笑气双阻断法把前列腺癌细胞系PC-3同步在不同的周期时相,应用流式细胞术检测同步化的效率.进而应用RT-PCR及Western blot方法检测不同周期时相Bcl-2和Bax在mRNA和蛋白水平的表达.结果 前列腺癌细胞系PC-3的M、G1、S和G2期细胞同步化效率分别为92.1%、87.0%、80.2%和75.9%;Bcl-2在不同周期时相均有表达且存在差异,G1期表达水平最高,S、M和G2期表达水平显著降低,mRNA和蛋白水平表达具有一致性;Bax在各周期的mRNA水平及蛋自水平表达没有明显差别.结论 细胞周期对凋亡相关基因Bel-2的表达有显著影响,对Bax无影响,对于采用Bcl-2途径治疗前列腺癌具有指导意义. Objective To investigate the differential expression of apoptosis associated gene Bcl-2 and Bax through cell cycle and its possible clinical meaning.Methods The prostate cancer cell line PC-3 was synchronized in M,G1,S and G2 phase using modified thymine deoxyriboside blockage and high pressure N2O technique.The efficiency of synchronization was detected by flow-cytometry.RT-PCR and western blot methods were used to examine the expression of Bcl-2 and Bax in mRNA and protein level.Results The synchronized rate of M,G1,S and G,phase were 92.1%,87.0%,80.2% and 75.9%respectively.Bcl-2 was constitutively expressed through the cell cycle,but both the mRNA and protein expression level of Bcl-2 were very high in the G1 phase,dramatically decreased in M,S and G2 phase.The expression level of Bax had no change through the cell cycle.Conclusions Cell cycle could influence the expression level of Bcl-2 significantly but not Bax.these might have some clinical relevance.
语种: 中文
原文出处: 查看原文
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内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/45254
Appears in Collections:北京大学第一临床医学院_泌尿外科_期刊论文

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作者单位: 1.北京大学第一医院泌尿外科北京大学泌尿外科研究所,100034
2.北京大学首钢医院泌尿外科
3.北京大学肿瘤医院北京肿瘤防治研究所

Recommended Citation:
李学松,龚侃,孙国锋,等. Bcl-2和Bax在前列腺癌不同周期时相的表达[J]. 中华外科杂志,2008,46(10):768-771.
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