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学科主题: 泌尿外科学
题名:
肾癌特异相关新基因GYLZ-RCC18的克隆及功能研究
其他题名: Renal cell carcinoma related novel gene, GYLZ-RCC18: cloning and functional studies
作者: 张强1; 陈琳1; 梁丽莉1; 席志军1; 丁义1; 佟明1; 张志文1; 李钟1; 郭应禄1
关键词: 肾肿瘤 ; ; 基因 ; GYLZ-RCC18 ; kidney neoplasm ; carcinoma ; gene ; GYLZ-RCC18
刊名: 中华医学杂志(英文版)
发表日期: 2002
卷: 115, 期:5, 页:746-749
文章类型: Journal Article
摘要: 目的克隆肾癌相关新基因GYLZ-RCC18的全长,并探讨其功能.方法应用SMART RACE技术克隆GYLZ-RCC18的全长,应用逆转录PCR的方法检测其在肾癌组织和细胞系中的表达特点,以及与肾癌组织分级、分期的关系,通过转染GYLZ-RCC18的反义寡核苷酸阻断其表达,观察其对肾癌细胞系生命特征的变化.结果 GYLZ-RCC18全长约3.5 kb(基因号:BE825133).GYLZ-RCC18在肾癌特异高表达,正常肾表达量极低甚至不表达,两者表达量差别1-9倍之间;GYLZ-RCC18在高分级、高分期肾癌中表达明显高于低分级、低分期肾癌的表达.转染反义基因可明显抑制GRC-1的生长、降低其增殖活性、减少核分裂、并诱导其持续性凋亡.结论本研究表明GYLZ-RCC18是肾癌发生发展密切相关的重要新的癌基因,它的表达与肾癌细胞的生存、增殖、抗凋亡发展密切相关,它的成功克隆为今后肾癌分子机制研究提供了新的理论依据. Objective To clone the full length of renal cell carcinoma (RCC) related novel gene GYLZ -RCC18 and study its function.Methods SMART RACE technology was used to clone the full length of GYLZ-RCC18. RT-PCR was used to detect its expression in renal cell carcinoma tissue at different s tages and grades. We transfected the antisense oligonucleotide of GYLZ-RCC18 t o renal cell carcinoma cell line, GRC-1, and analyzed proliferation activity, g rowth rate, apoptosis, and mortality changes.Results The full length of GYLZ-RCC18 (GenBank accession number: BE825133) cDNA was abo ut 3.5kb. GYLZ-RCC18 had a higher expression in higher grades and stage s of renal cell carcinoma than in lower ones. The expression of GYLZ-RCC18 in rena l cell carcinoma was much higher than in normal kidney. After the transfection of GYLZ-RCC18 antisense oligonucleotide, the mortality of GRC-1 increased sig nificantly, while proliferative activity and growth rate were substantially inhi bited at the same time. The antisense oligonucleotide induced apoptosis of GRC -1 through the entire observation time.Conclusion GYLZ-RCC18 is an important novel gene related to renal cell carcinoma. Overexp ression of this gene results in higher growth and proliferative activity and has an antiapoptosis effect on renal cell carcinoma cells. Transfection of the ant isense oligonucleotide may inhibit the generation and development of renal cell carcinoma.
语种: 中文
原文出处: 查看原文
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/45626
Appears in Collections:北京大学第一临床医学院_泌尿外科_期刊论文

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作者单位: 1.北京大学第一医院,北京大学泌尿外科研究所,北京,100034
2.福建省血液研究所,厦门,361001
3.美国芝加哥西北大学,美国

Recommended Citation:
张强,陈琳,梁丽莉,等. 肾癌特异相关新基因GYLZ-RCC18的克隆及功能研究[J]. 中华医学杂志(英文版),2002,115(5):746-749.
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