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学科主题: 泌尿外科学
题名:
新基因UCA1用于膀胱癌诊断的临床应用价值
其他题名: Evaluation of novel gene UCA1 as a tumor biomarker for the detection of bladder cancer
作者: 张争; 郝瀚; 张崔建; 杨新宇; 何群; 林健
关键词: 膀胱肿瘤 ; 肿瘤标记 ; 生物学 ; 尿路上皮癌抗原1 ; Urinary bladder neoplasms ; Tumor markers,biological ; Urothelial carcinoma antigen 1
刊名: 中华医学杂志
发表日期: 2012
DOI: 10.3760/cma.j.issn.0376-2491.2012.06.008
卷: 92, 期:6, 页:384-387
收录类别: 中国科技核心期刊 ; 中文核心期刊 ; CSCD
文章类型: Journal Article
摘要: 目的 评价新基因尿路上皮癌抗原1( UCA1)基因作为膀胱癌尿液肿瘤标志物用于膀胱癌诊断的临床应用价值.方法 用反转录PCR (RT-PCR)的方法检测2005至2009年180例膀胱癌患者(包括前期研究94例)、144例对照组人群(包括前期研究85例)尿液中UCA1 mRNA的表达,在2007年后收集的86例膀胱癌和59例对照组尿液标本的离心沉渣中加入RNA酶抑制剂,以提高RNA提取成功率.计算UCA1诊断膀胱癌的敏感度和特异度,并绘制受试者工作特征(ROC)曲线、计算曲线下面积(AUC).用x2检验比较116例同时做UCA1和核基质蛋白22( NMP22)检测的膀胱癌患者中UCA1与NMP22敏感度的差异,比较108例同时做UCA1和尿细胞学检查的膀胱癌患者中UCA1与尿细胞学敏感度的差异.结果 应用RNA酶抑制剂后尿液RNA提取成功率提高至95.4%.RT-PCR检测尿中UCA1的表达,对膀胱癌的诊断具有很高的特异度(92.4%,133/144)和较高的敏感度(84.4%,152/180),尤其对于G2~G3级浅表肿瘤更具诊断价值,敏感度分别达86.4%和92.3%.ROC曲线分析显示该基因对膀胱癌具有很好的预测价值,AUC=0.898 (95% CI:0.851 ~0.945).结论 UCA1用于膀胱癌诊断具有较高敏感性和高度特异性,且结果稳定,有望成为一种新的更好的尿液肿瘤标志物. Objective To evaluate the clinical utility of novel gene urothelial carcinoma antigen 1 ( UCA1 ) as a urinary tumor marker for the diagnosis of bladder urothelial carcinoma.Methods A cohort of 180 cases of bladder cancer (including 94 cases in previous study),144 cases of non-bladder-cancer individuals as control group (including 85 cases in previous study ) from 2005 to 2009 were recruited.Reverse transcription PCR (RT-PCR) of urinary sediments was performed to detect the expression of UCA1.RNasin was added to the urinary sediments colleted after 2007 from 86 cases of bladder cancer and 59 cases in control group to improve the quantity and quality of RNA isolation.The parameters of sensitivity,specificity,area under curve (AUC) of ROC and its 95% CI were calculated.x2 test was used to compare the sensitivity of UCA1 with NMP22 and cytology in 116 cases of bladder cancer with the parallel data of UCA1 and NMP22 and in 108 cases with the parallel data of UCA1 and cytology.Results 95.4% of RNA was isolated successfully from urinary sediments after the addition of RNasin UCA1 was highly specific (92.4%,133/144) and quite sensitive (84.4%,152/180) in the diagnosis of bladder cancer with a favorable AUC-ROC of 0.898 (95% CI:0.851 -0.945 ).It was especially valuable for superficial G2 -G3 patients (sensitivity:86.4%,92.3% ) at a high risk for muscular invasion.Conclusion With a high level of sensitivity and specificity,UCA1 is a promising urinary marker for the diagnosis of bladder cancer.
语种: 中文
原文出处: 查看原文
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内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/45657
Appears in Collections:北京大学第一临床医学院_泌尿外科_期刊论文

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作者单位: 100034,北京大学第一医院泌尿外科北京大学泌尿外科研究所

Recommended Citation:
张争,郝瀚,张崔建,等. 新基因UCA1用于膀胱癌诊断的临床应用价值[J]. 中华医学杂志,2012,92(6):384-387.
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