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学科主题: 泌尿外科学
题名:
腺伴随病毒介导人血管内皮生长因子受体KDR胞外区基因对裸鼠人膀胱癌生长及血管生成的影响
其他题名: Extracellular domain of kinase domain region mediated by adeno-associated virus inhibits growth and angiogenesis of bladder cancer in Balb-c mice
作者: 张志超1; 郭应禄1; 张智清1; 曾革非1; 张立国1; 徐春晓1
关键词: 血管内皮生长因子 ; 受体 ; 腺病毒伴随病毒 ; vascular endothelial growth factor ; receptor ; adeno-associated virus ; bladder cancer
刊名: 中华医学杂志(英文版)
发表日期: 2002
DOI: 10.3760/j.issn:0366-6999.2002.08.021
卷: 115, 期:8, 页:1209-1212
文章类型: Journal Article
摘要: 目的从原代培养的人脐静脉血管内皮细胞(HUVEC)提取细胞总RNA,采用逆转录PCR(RT-PCR)方法得到VEGF受体KDR全长胞外区cDNA 片段,检验其体内抗肿瘤血管生成的作用.方法将获得的受体基因克隆到AAV基因治疗载体pSNAV中,得到重组质粒pSNAV/KDR.重组质粒转染BHK细胞,加入辅助病毒后,得到表达目的蛋白的重组AAV.重组AAV表达的KDR具有与VEGF结合的活性,重组AAV感染人膀胱癌EJ细胞,皮下注射Balb-c裸鼠.Ⅷ因子免疫组化染色进行微血管密度测定.结果重组AAV感染人膀胱癌EJ细胞形成的肿瘤血管化程度明显低于对照组.结论重组AAV介导的KDR胞外区基因可有效的抑制裸鼠人膀胱癌组织的新生血管生成. Objective To verify whether the extracellular domain of kinase domain region (KDR) has anti-angiogenesis activity in vivo.Methods cDNA was cloned into adeno-associated virus (AAV) vector pSNAV and transfected to baby hamster kidney (BHK) cells. Recombinant AAV was obtained from the cell culture supernatant after adding helper virus. Recombinant AAV-infected human bladder cancer EJ cell line (EJ cells) were injected subcutaneously into Balb-c nude mice. Tumor specimens were removed from the mice, paraffin-embedded and sliced, then stained by immunohistochemistry. Microvessel density (MVD) was determined under a microscope.Results The tumor volume developed by EJ cells transfected with the extracellular domain of KDR was significantly smaller (1.70±0.18 cm3) compared with that in the control (5.62±0.67 cm3) (P<0.05), although tumor developed to be detectable on almost the same time (14.7±2.4 days vs 14.1±3.2 days). Further, MVD in the experimental group was lower than that in the control (41.3±4.8 vs 6.2±2.1, P<0.05).Conclusion The extracellular domain of KDR could be expressed in nude mouse bladder cancer tissue and inhibit tumor angiogenesis.
语种: 中文
原文出处: 查看原文
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内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/45663
Appears in Collections:北京大学第一临床医学院_泌尿外科_期刊论文

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作者单位: 1.北京大学第一医院泌尿外科研究所,北京,100034
2.中国预防医学科学院病毒学研究所,病毒基因工程国家重点实验室,北京,100052

Recommended Citation:
张志超,郭应禄,张智清,等. 腺伴随病毒介导人血管内皮生长因子受体KDR胞外区基因对裸鼠人膀胱癌生长及血管生成的影响[J]. 中华医学杂志(英文版),2002,115(8):1209-1212.
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