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过氧化物酶体增殖物激活受体γ激动剂对前列腺上皮细胞作用的研究
其他题名Effect of peroxisome proliferator-activated receptor γ agonist on prostate epithelial cells
郑兰斌; 赵亚元; 虞巍; 郭辉; 金杰
关键词前列腺增生 细胞系 Pparγ 半胱氨酸天冬氨酸蛋白酶 罗格列酮 Prostatic Hyperplasia Cell Line Ppar Gamma Caspases Rosiglitazone
刊名中华泌尿外科杂志
2010
DOI10.3760/cma.j.issn.1000-6702.2010.01.016
31期:1页:52-55
收录类别中国科技核心期刊 ; 中文核心期刊 ; CSCD
文章类型Journal Article
摘要目的 研究过氧化物酶体增殖物激活受体γ(PPARγ)在人类前列腺上皮细胞株RWPE-1的表达和对前列腺细胞凋亡过程的影响.方法 用免疫细胞化学和免疫荧光法检测PPARγ在RWPE-1细胞的表达.细胞分对照组和实验组,实验组细胞采用PPARγ激动剂罗格列酮处理,分别检测2组细胞中凋亡执行酶Caspase 3/7的活性,采用免疫细胞化学方法检测Bax表达,采用阳离子染料JC-1观察线粒体膜电位变化.采用SPSS 13.0软件对实验数据进行统计分析,组间均数比较采用独立样本t检验,α=0.05.结果 PPARγ在人类前列腺上皮细胞株RWPE-1表达,主要定位于细胞核和核周.实验组Caspase 3/7活性[(10 636±1032)RLU]明显高于对照组[(5936±620)RLU],差异有统计学意义(P<0.01).实验组Bax表达显著增加,对照组累计吸光度(A)值为6017±563,实验组为8250±694,2组比较差异有统计学意义(P<0.01).线粒体膜电位检查发现,对照组呈黄绿色荧光,实验组呈绿色荧光,表明实验组线粒体膜电位发生去极化改变.结论 PPARγ激动剂能够诱导前列腺上皮细胞凋亡,这一过程涉及Bax和线粒体膜电位改变,提示PPARγ可能通过调控前列腺细胞的凋亡过程参与BPH发病. Objective To assess the effect of peroxisome proliferator-activated receptor γ (PPARγ) agonist on prostate epithelial cells in vitro.Methods The expression of peroxisome proliferator-activated receptor γ(PPARγ) was studied by immunocytochemistry and immunofluorescence study.The RWPE-1 human prostate epithelial cell line was treated with PPARγ agonist rosiglitazone 100 μmol/L for 48 h.Analysis of apoptosis was performed by Caspase 3/7 activity assay.Mitochondria depolarization was measured by using the potential-sensitive color,JC-1.The expression of apoptosis-related proteins-Bax was investigated by immunohistochemistry.Results PPARγ mainly located in nucleus and perinucleus.RWPE-1 cell line treated with PPARγ agonist rosiglitazone showed higher Caspase 3/7 activity (10636±1032 RLU) than in control (5936±620 RLU),P<0.01 and significantly upregulated Bax level (8250±694 vs.6017±563)than in control group,P<0.01.In addition,mitochondrial membrane potential was depolarized in rosiglitazone treated cells.Conclusions PPARmay play important roles in the pathophysiology of BPH.The mechanism might be that PPARγ regulates cell apoptosis.It is suggested that the mitochondrial and Bax pathway might be involved in signaling PPARγ induced cell apoptosis.
语种中文
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文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/45691
Collection北京大学第一临床医学院_泌尿外科
作者单位北京大学泌尿外科研究所,北京大学第一医院泌尿外科,100034
Recommended Citation
GB/T 7714
郑兰斌,赵亚元,虞巍,等. 过氧化物酶体增殖物激活受体γ激动剂对前列腺上皮细胞作用的研究[J]. 中华泌尿外科杂志,2010,31(1):52-55.
APA 郑兰斌,赵亚元,虞巍,郭辉,&金杰.(2010).过氧化物酶体增殖物激活受体γ激动剂对前列腺上皮细胞作用的研究.中华泌尿外科杂志,31(1),52-55.
MLA 郑兰斌,et al."过氧化物酶体增殖物激活受体γ激动剂对前列腺上皮细胞作用的研究".中华泌尿外科杂志 31.1(2010):52-55.
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